1a 2a and 7a - 123doc

Báo cáo khoa học: The ATPase activities of sulfonylurea receptor 2A and sulfonylurea receptor 2B are inﬂuenced by the C-terminal 42 amino acids doc

Ngày tải lên : 06/03/2014, 11:20

... NBD2A (2A, , n = 7) and NBD2B (2B, s, n = 7) The lines are ﬁtted to the Michaelis–Menten equation with estimated Vmax values of 37, 26, and 31 nmol PiÆminÆmg)1, and Km values of 769, 556, 882 and ... full-length SUR2A and SUR2B MALDI-TOF MS analysis conﬁrmed their identities For simplicity, we refer to MBP–NBD fusion proteins hereafter as NBD1, NBD2A (NBD2 of SUR2A), NBD2B (NBD2 of SUR2B), and NBD2-DC ... and NBD2-DC ATP hydrolysis by NBDs NBD1 and NBD2A displayed higher ATPase activity than NBD2B (Fig 2A; Table 1), with NBD1 having ATPase activity of SUR2A and SUR2B A MBP–SUR2 NBDs 250 150 250...

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Báo cáo khoa học: Lithium increases PGC-1a expression and mitochondrial biogenesis in primary bovine aortic endothelial cells ppt

Ngày tải lên : 23/03/2014, 09:20

... biogenesis and mass within cells and tissues reﬂect changes in energy demand and hormonal status, and depend mainly on changes in the activity and ⁄ or expression of the transcription factors NRF1 and ... factors NRF1 and NRF2b and coactivator PGC -1a [25,26] In addition, we showed that two different signaling cascades known to regulate PGC -1a expression, inactivation of Akt [46] and activation ... coactivator PGC -1a (A) Lithium increases the levels of PGC -1a mRNA BAEC were treated for 36 h with 10 mM NaCl, 10 mM LiCl, mM VPA or lM indirubin prior to RNA extraction and the levels of PGC -1a and PRC...

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Bai luyen tap nhom 1A, 2A

Ngày tải lên : 19/07/2014, 06:00

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Báo cáo y học: "Prediction of prognostic biomarkers for Interferon-based therapy to Hepatitis C Virus patients: a metaanalysis of the NS5A protein in subtypes 1a, 1b, and 3a" doc

Ngày tải lên : 12/08/2014, 04:20

... S1, Table S1, and Table S2 Figure S1: Distance-based tree of the ISDR and V3 regions for subtypes 1a, 1b, and 3a 2A: V3 1b nj tree 2B: ISDR 3a nj tree 2C: V3 3a nj tree 2D: NS5A 1a nj tree The ... between responders and non-responders in subtypes 1b and 3a There were statistically significant variations in the IRRDR regions (2326, 2342 and 2349 in subtype 1a; 2332, 2348 and 2383 in subtype ... our current knowledge and understanding of biological and molecular entities Pattern recognition and representation of motifs is a fundamental problem in bioinformatics and bioinformatics for...

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Báo cáo y học: "Dephosphorylation of CDK9 by protein phosphatase 2A and protein phosphatase-1 in Tat-activated HIV-1 transcription" doc

Ngày tải lên : 13/08/2014, 09:21

... the addition of mM EDTA and (32P) phosphorylated CDK9 was used as a substrate for PP1 or PP2A (Fig 1A, lane 1) While PP2A efficiently dephosphorylated CDK9 (Fig 1A, lanes and 5), PP1 was approximately ... Flag-tagged CDK9 (lanes and 3), CDK9 T186A mutant (lanes and 5) and CDK9 C8A mutant (lanes and 7) Cells were labeled with (32P) orthophosphate in the absence (lanes 2, and 6) and in the presence ... serine-threonine phosphatases, protein phosphatase 2A (PP2A) and protein phosphates-1 (PP1) were implicated in the regulation of HIV-1 transcription PP2A and PP1 are a general phosphatases that belong...

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Báo cáo y học: " Kinetics of hepatitis C virus RNA load during pegylated interferon alpha-2a and ribavirin treatment in naïve genotype 1 patients" potx

Ngày tải lên : 13/08/2014, 13:20

... Peginterferon-alpha2a and ribavirin combination therapy in chronic hepatitis C: a randomized study of treatment duration and ribavirin dose Ann Intern Med 2004, 140(5):346-355 Halfon P, Khiri H, Tran A, Penaranda ... course of combination (Peg-IFN alpha -2a plus ribavirin) and just before starting interferon and ribavirin therapy for chronic HCV infection, at the Hepatology and Gastroenterology Department of ... to week 2, 4, 8, and 12 of treatment, week point time tend to be better than other time points for predicting SVR, with a PPV of 91% and a NPV of 89% and a very good sensitivity and specificity...

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Báo cáo y học: "Effects of ischemic pre- and postconditioning on HIF-1a, VEGF and TGF-b expression after warm ischemia and reperfusion in the rat liver" ppsx

Ngày tải lên : 13/08/2014, 13:20

... examine how ischemia, with or without IPC and IPO, affects the expression of HIF -1a and the target genes VEGF and TGF-b1, in rodent liver Methods The surgical and experimental protocols were approved ... ischemic pre- and postconditioning on HIF -1a, VEGF and TGF-b expression after warm ischemia and reperfusion in the rat liver Comparative Hepatology 2011 10:3 • Inclusion in PubMed, CAS, Scopus and Google ... Jansen Pharma, Birkerød, Denmark) and mg/kg Midazolam s.c (Dormicum, La Roche, Basel, Switzerland) and placed on a heated pad A midline laparotomy was performed and total hepatic ischemia was accomplished...

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35 BÀI TẬP NHÓM 1A, 2A, Al

Ngày tải lên : 29/01/2015, 01:00

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let''''s go 2A unit 2 let''''s read and let''''s listen

Ngày tải lên : 30/09/2013, 02:10

... Warm up and review: H·y nèi çc bøc tranh sau víi çc tõ t¬ng øng? Tranh Tõ cat Tranh hat Tranh Warm up and review: H·y nèi çc bøc tranh sau víi çc tõ ... Lesson 16: Unit 2: Let’s read and let’s listen I) Let’s read bed hen net red pen pet Ted ten wet Wednesday, October, 13rd, 2010 Lesson 16: Unit 2: Let’s read and let’s listen I) Let’s read ... read 1.Word Families -ed -en -et Wednesday, October, 13rd, 2010 Lesson 16: Unit 2: Let’s read and let’s listen I) Let’s read 1.Word Families 2.Can you read? Ted is in the red bed The ten pens...

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Tài liệu Báo cáo Y học: Identiﬁcation of syntaxin-1A sites of phosphorylation by casein kinase I and casein kinase II ppt

Ngày tải lên : 22/02/2014, 04:20

... syntaxin -1A that are phosphorylated by CKI and CKII Initially two truncated mutants of syntaxin -1A (1–190 and 191–265) were subjected to in vitro kinase assays Syntaxin -1A (1–190), but not syntaxin -1A ... Phosphorylation of syntaxin -1A by CKI and CKII (Eur J Biochem 269) 911 Fig Phosphorylation of syntaxin -1A (1–265, 1–190, 191–265) by CKI and CKII In order to map the CKI and CKII phosphorylation ... (Thr21) and CKII (Ser14) on syntaxin -1A are summarized in Fig with the masses of the peptides identiﬁed To summarize, we have shown that CKIa and CKII phosphorylate syntaxin -1A on Thr21 and on...

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Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt

Ngày tải lên : 07/03/2014, 05:20

... IL) and BSA as standard Rabbit polyclonal antibodies for TIPRL (A300–663A), PP2Ac (A300– 7 32A) and a4 (A300–4 71A) were used in immunoprecipitation assays, and antibody for murine MafB (A300–6 12A) , ... a novel PP2A heterotrimer J H C Smetana and N I T Zanchin A GST fusion: PP2Acα PP2Acβ α β I B I PP4c B I B PP6c I PP2AcCT B I B GST I B Anti-His GST fusion: PP2Acα/β αβ PP6c PP4c PP2AcCT Coomassie ... TIPRL, PP2Ac and a4 (A) GST, GST–TIPRL, GST–PP2Aca and His–a4 were expressed separately in E coli and His–PP2Aca was coexpressed with His–a4 in E coli Bound proteins were resolved by SDS ⁄ PAGE and...

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Class 1A National Insurance contributions on Car and Fuel Benefits pptx

Ngày tải lên : 07/03/2014, 11:20

... benefits are liable for Class 1A NICs • when liability for Class 1A NICs arises, and • how you report and pay Class 1A NICs How are Class 1A NICs worked out? Class 1A NICs are worked out in one ... circumstances Class 1A NICs are not due on car and fuel benefits Part – Exceptions from Class 1A National Insurance contributions 15 When are Class 1A NICs not payable? You not have to pay Class 1A NICs ... Class 1A National Insurance contributions (NICs) are due on most taxable benefits, including car and fuel benefits CWG5(2012) Class 1A NICs on benefits in kind is the main guide about Class 1A NICs...

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Báo cáo khoa học: The stop transfer sequence of the human UDPglucuronosyltransferase 1A determines localization to the endoplasmic reticulum by both static retention and retrieval mechanisms docx

Ngày tải lên : 07/03/2014, 16:20

... BamHI site and UGT1A6 nucleotides 1467– 1482 and a 3¢ primer 5¢-CTCGAGTCAATGGGTCTTG GATTTGTG-3¢ encoding for the last six residues of human UGT1A6 (1593–1576) followed by a stop codon and XhoI ... protein [15] and a unique N-terminal end UGT1A proteins are predicted to be type I membrane proteins of the ER with a glycosylated lumenal domain It has also been reported that UGT2B7 and UGT1A6 were ... C-terminal 43 amino acids of human UGT1A stop transfer sequence, TMD ⁄ CT coding sequence was ampliﬁed by PCR using UGT1A6 cDNA [13] (a member of UGT1A family) as template and two primers, a 5¢ primer...

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Báo cáo khoa học: Salicylic acid and the hypersensitive response initiate distinct signal transduction pathways in tobacco that converge on the as-1-like element of the PR-1a promoter pot

Ngày tải lên : 23/03/2014, 15:21

... similarly constructed 35S[PR -1a] transgene (Fig and data not shown) Tobacco plants transformed with the )1533PR1a[W38-1::PR -1a] construct were infected Ó FEBS 2003 Analysis of PR -1a gene regulation (Eur ... PR -1a, PR-1b and PR-1c proteins by SDS gel electrophoresis (Fig and data not shown) When put under control of the CaMV 35S RNA promoter in transgenic tobacco plants, the chimeric W38-1::PR -1a ... by the PR -1a gene, and differs by only six amino acids from the PR -1a preprotein in the N-terminal moiety of the protein Most importantly, the W38-1 pseudogene part of the W38-1::PR -1a chimeric...

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Báo cáo khoa học: Inhibitors of protein phosphatase 1 and 2A decrease the level of tubulin carboxypeptidase activity associated with microtubules pptx

Ngày tải lên : 23/03/2014, 15:21

... the assembled and nonassembled tubulin fractions from cells treated and nontreated with lM OA was concentrated on phosphocellulose columns (for details see Materials and methods) and enzyme activity ... microtubule-stabilizing buﬀer, and homogenized to isolate the microtubular and soluble tubulin fractions Both fractions were concentrated and assayed as described in Materials and methods Carboxypeptidase ... only calyculin A and cantharidin, two well-known inhibitors of PP1 and PP2A [23,24], showed effects similar to that of OA (Fig 4) Deltamethrin, a speciﬁc inhibitor of PP2B [25], and phenylarsine...

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Báo cáo khoa học: Autophosphorylation of heme-regulated eukaryotic initiation factor 2a kinase and the role of the modiﬁcation in catalysis doc

Ngày tải lên : 28/03/2014, 23:20

... (2177130 and 21117501) and T.S (17101002), and Special Education and Research Expenses from the Ministry of Education, Culture, Sports, Science, and Technology of Japan References Trypsin digestion and ... is the marker band Only in the presence of heme (lanes and 4) were interactions between His6NTD and KD detected In the absence of heme (lanes and 3), interactions between His6-NTD and KD were not ... LAS-3000 (Fujifilm, Tokyo, Japan) and quantified with multi-gauge software (Fujifilm) To calculate specific activity and KD recovery values, the ratios of P-eIF2a over eIF2a, and KD over His6-NTD were obtained...

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smart grammar and vocabulary 2a

Ngày tải lên : 29/03/2014, 01:23

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Báo cáo khoa học: The enzymatic activity of SR protein kinases 1 and 1a is negatively affected by interaction with scaffold attachment factors B1 and 2 pot

Ngày tải lên : 30/03/2014, 01:20

... 11 and 12), albeit with different afﬁnities SAFB1 binds tightly to SRPK1a and almost as tightly to SRPK1 (compare lanes and 1) and the same holds true for the GST–SAFB1CDRE protein (lanes and ... FLAG–SRPK1a FLAG–SRPK1 FLAG–SRPK1a FLAG–SRPK1 FLAG–SRPK1a FLAG–SRPK1 FLAG–SRPK1a Eluate FLAG–SRPK1 Fig Binding of the GST–SAFB1/2 proteins on immobilized FLAG–SRPK1 /1a proteins FLAG–SRPK1 and FLAG–SRPK1a ... phophorylated by SRPK1a [37] (and our unpublished observations) As shown in Fig 1C, SRPK1a phosphorylates bacterially Results FLAG–SRPK1a activity is inhibited in vitro by SAFB1 SRPK1a interacts with...

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Báo cáo khoa học: Transcription factor speciﬁcity protein 1 (SP1) and activating protein 2a (AP-2a) regulate expression of human KCTD10 gene by binding to proximal region of promoter pot

Ngày tải lên : 30/03/2014, 02:20

... promoter activity of KCTD10; and SP1 and AP -2a can regulate the promoter activity and endogenous KCTD10 expression oppositely through binding this region SP1 and AP -2a regulate KCTD10 Results Analysis ... from Invitrogen to suppress the endogenous expression of Sp1 and AP -2a; pCMV–Sp1 and pCMV–AP -2a were used to overexpress Sp1 and AP -2a The cultured cells were transfected using LipofectamineÔ ... University Journal compilation ª 2009 FEBS R Liu et al SP1 and AP -2a regulate KCTD10 Roles of Sp1 and AP -2a in the regulation of the promoter activity and the expression of human KCTD10 Fig Transcription...

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Báo cáo sinh học: " Neopterin production and tryptophan degradation during 24-months therapy with interferon beta-1a in multiple sclerosis patients" docx

Ngày tải lên : 18/06/2014, 19:20

... IFNb -1a at a dose of 44 or 22 mcg, sc three times weekly for years To minimize adverse effects, IFNb -1a was titrated as follows: 8.8 mcg at weeks and of therapy, 22 mcg at weeks and 4, and, for ... as an internal standard The coefficient of variation of intra- and interassay determinations for trp and kyn was below 5% Recovery of trp and kyn was determined by measuring trp and kyn in 20 μl ... Isernia, L’Aquila, Messina, Roma, and Trieste), in collaboration with the University of Innsbruck in Austria and the National Institute of Biological Standards and Control in London, UK The study...

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