17 estradiol and estrogen receptor beta
Báo cáo khoa học: Dehydroepiandrosterone inhibits the proliferation and induces the death of HPV-positive and HPV-negative cervical cancer cells through an androgen- and estrogen-receptor independent mechanism pptx
Ngày tải lên :
16/03/2014, 00:20
... estrogen and androgen, because use of antagonists to androgen and estrogen receptors (flutamide and ICI 182,780, respectively), and letrozol, an inhibitor of the aromatase responsible for converting androgen ... or estradiol In order to assess this, antagonists to androgen and estrogen receptors (flutamide and ICI 182,780, respectively), and an inhibitor of the aromatase responsible for conversion of androgen ... testosterone and then to estradiol by the P450 aromatase It has been shown that approximately 35% of cervical carcinomas express aromatase [37] and that DHEA binds to the androgen receptor and estrogen receptors...
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Báo cáo y học: " mRNA expression profiles show differential regulatory effects of microRNAs between estrogen receptor-positive and estrogen receptor-negative breast cancer" pdf
Ngày tải lên :
09/08/2014, 20:20
... differential expression between ER- and ER+ samples (b) Expression levels of Ago1, Ago2, Dicer, and TRBP in ER+ (red) and ER- (green) samples The mean and the standard deviation of the expression ... methods, and RE-changing miRNA identification thresholds and obtained consistent results Moreover, we examined the expression levels of genes in the miRNA biogenesis pathway and found that Ago1 and ... PicTar algorithm (b) miRanda algorithm (c) Intersection of miRanda and PITA (d-f) TargetScan algorithm where the site is conserved (d), the site context score is above -0.20 (e), and all potential...
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Báo cáo y học: "Expression of aromatase and estrogen receptor alpha in chondrosarcoma, but no beneficial effect of inhibiting estrogen signaling both in vitro and in vivo" ppt
Ngày tải lên :
13/08/2014, 15:21
... (I and J, respectively) ESR1 protein expression in the JJ012 and CH2879 chondrosarcoma cell lines (K and L) Magnification 200× the estrogen precursor androstenedione and the nonaromatizable androgen ... with estrogen signaling could inhibit chondrosarcoma growth We aimed to validate and expand our previous in vitro data by measuring the effects of estrogens, androgens, tamoxifen, fulvestrant, and ... enzyme responsible for the conversion of androstenedione and androgens to estrogens, was expressed in 86% (38 out of 44) and 93% (25 out of 27) of the central and peripheral chondrosarcomas respectively...
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Báo cáo khoa học: An estrogen receptor a suppressor, microRNA-22, is downregulated in estrogen receptor a-positive human breast cancer cell lines and clinical samples pptx
Ngày tải lên :
22/03/2014, 21:20
... reduce ERa and SP1 protein levels in pancreatic cancer cells [33] Estrogen receptors (mainly ERa and ERb) constitute a group of ligand-activated nuclear receptors that are activated by estrogen ... Materials and methods MicroRNA-22 and estrogen receptor a RNA isolation and quantitative RT-PCR detection Total RNAs from cultured cells were isolated using TRI Reagent (Sigma, St Louis, MO, USA) and ... is the median; the upper and lower limits of the box are the 75th and 25th percentiles, respectively, and the vertical bars above and below the box indicate the maximum and minimum values The solid...
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báo cáo hóa học:" Positron emission tomography in ovarian cancer: 18F-deoxy-glucose and 16α-18F-fluoro-17β-estradiol PET" docx
Ngày tải lên :
20/06/2014, 07:20
... 16α-[18F]fluoro-17β -estradiol (FES) is a radiopharmaceutical that binds to the estrogen receptor (ER), thereby demonstrating the existence of this receptor [16] FES can help diagnose ER-positive breast cancer and ... Fujibayashi Y, Itoh H: In vivo imaging of estrogen receptor concentration in the endometrium and myometrium using FES PET – influence of menstrual cycle and endogenous estrogen level Nucl Med Biol 2007, ... regions; the maximum SUV was 2.5 and 2.1 (figure 2), respectively After resection, both the leiomyoma and ovarian cancer were found to be focally positive for estrogen receptor- α (ER-α) (figure 3)...
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Báo cáo y học: " A folate receptor beta-specific human monoclonal antibody recognizes activated macrophage of rheumatoid patients and mediates antibodydependent cell-mediated cytotoxicity" ppsx
Ngày tải lên :
12/08/2014, 15:22
... autoimmune diseases and FRb-positive tumors/leukemia Materials and methods Expression of recombinant folate receptor beta Human folate receptor beta (FRb) fragment including amino acids 22 to 236 (the ... FITC: fluorescein isothiocyanate; FR: folate receptor; FRβ: folate receptor beta; GPI: glycosylphosphatidylinositol; hFRβ: human folate receptor beta; KD: equilibrium dissociation constant; mAb: ... N-terminus and six histidines on the C-terminus Purity of rFRb was examined with 4% to 12% NuPAGE Functional analysis of recombinant folate receptor beta rFRb was allowed to bind to Ni-NTA beads and...
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Báo cáo y học: " Interactions Between Estrogen- and Ah-Receptor Signalling Pathways in Primary Culture of Salmon Hepatocytes Exposed to Nonylphenol and 3,3'''',4,4''''-Tetrachlorobiphenyl (Congener 77)" ppsx
Ngày tải lên :
13/08/2014, 13:20
... reproductive and developmental processes; firstly as an interface between the egg and sperm, and secondly as an interface between the embryo and its environment [4,5] Vitellogenesis and zonagenesis are estrogen ... interactions between estrogen- and Ah -receptor signalling pathways Chem Res Toxicol 2007, 20:474-488 Matthews J, Gustafsson J-Å: Estrogen signaling: a subtle balance between ER alpha and ER beta Mol Interv ... oestrogen receptor signalling by association with the activated dioxin receptor Nature 2003, 423:545-550 Shipley JM, Waxman DJ: Aryl hydrocarbon receptor- independent activation of estrogen receptor- dependent...
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Báo cáo y học: "Discovery of estrogen receptor α target genes and response elements in breast tumor cells" ppsx
Ngày tải lên :
14/08/2014, 14:21
... Background Estrogens are involved in a number of vertebrate developmental and physiological processes Human and animal studies have revealed the roles of estrogen receptor (ER) in female and male ... of estrogen receptor biological activity J Steroid Biochem Mol Biol 2003, 86:387-391 Ali S, Coombes RC: Estrogen receptor alpha in human breast cancer: occurrence and significance J Mammary Gland ... Estrogen receptor pathways to AP-1 J Steroid Biochem Mol Biol 2000, 74:311-317 Saville B, Wormke M, Wang F, Nguyen T, Enmark E, Kuiper G, Gustafsson JÅ, Safe S: Ligand-, cell-, and estrogen receptor...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 1
Ngày tải lên :
09/09/2015, 10:19
... systematic estrogen available to estrogen receptor either through reducing the overall estrogen level or by blocking the estrogen receptor (Yager and Davidson, 2006) Although antiestrogen therapy ... On the other hand, other members of the nuclear receptors such as vitamin D receptor, thyroid hormone receptor and retinoic acid receptor recognize direct repeats separated by 3, and nucleotides ... acid receptors (RARs) and vitamin D receptors (VDR) (Gronemeyer et al., 2004) The intricate interplay between PPARs, RXRs and other nuclear receptors further adds complexity to the biological landscape...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 2
Ngày tải lên :
09/09/2015, 10:19
... MDA-MB-231 and T47D were selected to be exposed to various PPARγ ligands at different doses The three cell lines express different levels of endogenous PPARγ receptor (Figure 2D) Prostaglandin J2 and ... ligands on NHE1 protein is not drug specific, but is conserved in both the endogenous and synthetic PPARγ ligands.The difference in extent of NHE1 repression by 15d-PGJ2 and synthetic PPARγ ligands ... synthetic PPARγ ligands which are clinically available for treatment of type diabetes Members of TZDs include troglitazone, rosiglitazone, and pioglitazone, marketed as Rezulin, Avandia and Actos respectively...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 4
Ngày tải lên :
09/09/2015, 10:19
... PPARγ, and thus masking the effects of exogenous PPARγ ligands added Other than the above mentioned components that could potentially modulate the effect of PPARγ ligand on NHE1 expression, 17β -estradiol ... control 3B1.2 Estrogen blocks PPARγ-mediated down-regulation of NHE1 in CS serum condition As mentioned above, it was reported that activated estrogen receptor can bind to PPRE of PTEN and negatively ... three PPARγ ligands tested (Figure 18C) In this section, we have further confirmed that PPARγ-mediated repression of NHE1 protein and mRNA are inhibited by estrogen 127 Figure 18: Estrogen blocks...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 6
Ngày tải lên :
09/09/2015, 10:19
... cardiac, and adipose tissue development." Mol Cell 4(4): 585-595 Barkhem, T., B Carlsson, et al (1998) "Differential response of estrogen receptor alpha and estrogen receptor beta to partial estrogen ... proliferator-activated receptor- delta accelerates intestinal adenoma growth." Nat Med 10(3): 245-247 Hall, J M and D P McDonnell (1999) "The estrogen receptor beta- isoform (ERbeta) of the human estrogen receptor ... "Design and synthesis of aryl diphenolic azoles as potent and selective estrogen receptor- beta ligands." J Med Chem 47(21): 5021-5040 Malo, M E and L Fliegel (2006) "Physiological role and regulation...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 3
Ngày tải lên :
09/09/2015, 10:19
... correlation between the PPARγ receptor level and the sensitivity to PPARγ ligands in different breast cancer cell lines strongly implies a regualtory link between PPARγ activity and its anti-tumor effects ... ligands To further accertain that the colonogenic inhibition by 15d-PGJ2 is common in other PPARγ expressing breast cancer cell lines, MDA-MB-231 and T47D cells were also subjected to 1µM and ... cells and only a 36% down-regulation in T47D cells (Figure 14D) Interesetingly, the extent of inhibition on cologenic ability of different breast cancer cells mirror-imaged the level of PPARγ receptor...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 5
Ngày tải lên :
09/09/2015, 10:19
... AF1 and AF2 domains of ERα (Fawell et al., 1990) Raloxifene acts as an estrogen antagonist in both breast and uterus (Mitlak and Cohen, 1999) by blocking the accessibility of AF2 domain in ERα receptor ... This result suggests that the both ERα and PPARγ were active in regular serum condition, probably due to the high concentration of estrogen and prostaglandins present Upon inhibition of ERα by ... (Thiebaud and Secrest, 2001) To ascertain that both fulvestrant and raloxifene acted as ERα antagonist in our system, the protein expression of two ERα target genes, progesterone receptor and 145...
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AP 2 regulates estrogen receptor mediated long range chromatin interactions and gene transcription
Ngày tải lên :
09/09/2015, 10:20
... transcription machinery and transcriptional activation Nuclear receptor co-repressor (N-CoR) and silencing mediator of retinoid and thyroid receptors (SMRT) are the best characterized and structurally ... have demonstrated that ERα and its co-regulators are recruited and assembled in sequential- and cyclical-manner at the promoters of TFF-1 and CTSD in response to estrogen, and this is necessary to ... Gustafsson, 2001) Moreover, differences in ERα and ERβ ligand binding domain confer their specificity and affinity for natural and synthetic ligands (Barkhem et al, 1998) ER dimerization results...
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Redox regulation of estrogen receptor alpha and sodium hydrogen exchanger 1 gene expression by hydrogen peroxide
Ngày tải lên :
10/09/2015, 15:49
... 16 1.3 ESTROGEN RECEPTOR 17 1.3.1 Estrogen and estrogen receptors: Introduction 17 1.3.2 Estrogen receptor and genomic signaling 20 1.3.3 Membrane and cytoplasmic ... Membrane and cytoplasmic ER signaling 21 1.3.4 Regulation of estrogen receptors 23 1.3.5 Estrogen and estrogen receptors in human breast cancer 27 v 1.4 THE SODIUM HYDROGEN EXCHANGER ... 1.3.1 Estrogen and estrogen receptors: Introduction Estrogens are steroid hormones produced primarily in the ovaries of pre-menopausal, non-pregnant women They are converted from testosterone to estradiol...
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Differential global effects of selective estrogen receptor modulators on estrogen receptor binding and transcriptional regulation
Ngày tải lên :
14/09/2015, 14:05
... breast cancer (Ali and Coombes 2000) Estrogen receptor belongs to the family of steroid receptor and is activated by the hormone estrogen Estrogen exerts its effects through growth and proliferation ... and thymus tissues In liver tissue, only ERα is present Since estrogen receptors are distributed in different levels in many tissues and estrogen enhances the activity of estrogen receptor, estrogen ... tissue When estrogen binds to an estrogen receptor, the receptor dissociates from its cytoplasmic chaperones, the receptor- associated proteins The hormone– Lee Yew Kok PhD Thesis receptor complex...
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Dual activation of estrogen receptor a and aryl hydrocarbon receptor by the prenylflavone, icaritin restrict breast cancer cell growth and destabilize estrogen receptor a protein
Ngày tải lên :
04/10/2015, 17:05
... estrogens yields 2hydroxyestrogens, 4-hydroxyestrogens and 16α-hydroxyestrogens (catechol estrogens) 4-hydroxyestrone and 16α-hydroxyestradiol are carcinogenic 1.2.3 Estrogens and mammary gland ... triphosphates estrone 17β -estradiol estriol Epimedium brevicornum estrogen receptor estrogen receptor alpha estrogen receptor beta Eagle’s minimum essential medium estrogen response element fetal ... estrogen, namely 17β -estradiol (estradiol) , estrone (E1) and estriol (E3) However, the most prevalent form of human estrogen is estradiol Estrogens are produced and secreted by the theca and granulose...
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Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt
Ngày tải lên :
18/02/2014, 18:20
... reported a positive feedback loop between PPARb and prostaglandin E2 through which PPARb promotes COX-2 expression and prostaglandin E2 synthesis, and subsequent activation of cystolic phospholipase ... °C for 30 s, 60 °C for 30 s and 72 °C for 60 s, and 35 cycles were used The PCR condition of CERK was as follows: 95 °C for 30 s, 55 °C for 30 s and 72 °C for 60 s, and 35 cycles were used The ... s and 72 °C for 60 s, and 28 cycles were used PCR products were separated by electrophoresis on 2% agarose gels and visualized by ethidium bromide staining The relative intensity of the gel bands...
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Báo cáo khoa học: The ATPase activities of sulfonylurea receptor 2A and sulfonylurea receptor 2B are influenced by the C-terminal 42 amino acids doc
Ngày tải lên :
06/03/2014, 11:20
... (site and site 2) that comprise the Walker A and Walker B motifs of one NBD and the linker domain of the other In the absence of Mg2+, there is little difference in ATP block of Kir6.2 ⁄ SUR2A and ... , n = 7) and NBD2B (2B, s, n = 7) The lines are fitted to the Michaelis–Menten equation with estimated Vmax values of 37, 26, and 31 nmol PiÆminÆmg)1, and Km values of 769, 556, 882 and 340 lM, ... mixture of NBD1 and either NBD2A (s, n = 4) or NBD2B ( , n = 4) The solid lines are fitted to the Michaelis–Menten equation with estimated Km values of 995 and 878 lM, and Vmax values of 31 and 27 nmol...
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