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Tài liệu Building Entrance Terminals Enclosed, 5-Pin Protection with KRONE® Series 2 Blocks pdf
... 9/05 • 101469 Building Entrance Terminals Building Entrance TerminalsEnclosed, 5-Pin Protection with KRONE®Series 2 Blocks 2www.adc.com • +1-952-938-8080 • ... (-40 ˚ to 176 ˚ F)Indoor -20 ˚ to 70 ˚ C (-4 ˚ to 158 ˚ F)Building Entrance TerminalsEnclosed, 5-Pin Protection with KRONE®Series 2 Blocks Features: • 5-pin protection devices safeguard ... and equipment from voltagesurges and spikes • Lockable metal enclosure with Telcooverride cover and a splicing chamber with the choice of either 710 or MS2®spliceconnectors • 5-pin protectors...
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Tài liệu Báo cáo khoa học: Association of mammalian sterile twenty kinases, Mst1 and Mst2, with hSalvador via C-terminal coiled-coil domains, leads to its stabilization and phosphorylation doc
... hSalvador interact via their C -terminal coiled-coil domains. (A–D) WT, C-terminally or N-terminally truncated mutantsof flag-Sav cDNA were cotransfected with or without WT or C-terminally truncated mutants ... indica-ted with arrows while the position of flag-Sav is marked with arrowheads. (B) Theposition of CrmA-DQMD (lane 6) is marked with an asterisk (*). Each experiment wasperformed at least twice with ... homo-multimerizeindependently of its C -terminal coiled-coildomain. (A,B) HA-tagged Sav cDNA was co-transfected with either WT or C-terminallytruncated mutants of flag-Sav cDNA or with HA-PPIA or flag-CrmA-DQMD...
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Tài liệu Báo cáo khoa học: The distinct nucleotide binding states of the transporter associated with antigen processing (TAP) are regulated by the nonhomologous C-terminal tails of TAP1 and TAP2 ppt
... immunoprecipitated with antibody MAC 394 and analyzed by Western blots probed for the C -terminal tail of TAP1 with antiserum D90 (TAPwt and 1–2V1) or the C- terminal tail of TAP2 with antiserum 116/5 ... X-100 and incubated with differentnucleotide agaroses. Bound proteins were eluted with SDS-samplebuffer and analyzed in Western blots probed for the C -terminal tailof TAP1 with antiserum D90 ... combination with TAP2DV were lysed with 1% Triton X-100. Cell extracts were separated by SDS/PAGE andblotted onto nitrocellulose membranes. Western blots were probed with antiserum D90 (C-terminal...
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Tài liệu Báo cáo Y học: The Ikaros family protein Eos associates with C-terminal-binding protein corepressors pptx
... 5891C -terminal domain of Eos, CtBP can also bind sites withinthe N -terminal domain of Eos.TRPS1 interacts with CtBPThe TRPS1 protein is a multi-(zinc finger) protein thatcontains two C -terminal ... protein with six zinc fingers that comply with theKru¨ppel C2-H2 consensus. The N -terminal fingers areinvolved in sequence-specific DNA binding [4], while thetwo zinc fingers that form the C -terminal ... motif in Eos supportsonly weak binding of CtBP, we show that additionalregions within the N -terminal and C -terminal regions ofEos also contact CtBP, and, taken together, the results ofGST-pulldown,...
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Báo cáo khoa học: Unconventional translation initiation of human trypsinogen 4 at a CUG codon with an N-terminal leucine A possible means to regulate gene expression pdf
... of trypsinogen 4 with a C -terminal GFP fusion protein; GFP-p72MT4, p72 form of trypsinogen 4 with anN -terminal GFP fusion protein; p28LT4-GFP, p28 form of trypsinogen 4 with a deleted ATG()44) ... digestion; N -terminal sequencingrevealed the presence of both trypsin 4 with the N-ter-minal isoleucine and intact Isoform B starting with leucine (Fig. 1D).Searching for a protease with potentialprocessing ... analyzed by N -terminal aminoacid sequencing (Fig. 2C,D). Western blots, together with the N -terminal amino acid sequences of trypsino-gens isolated from 6 · 106cells transfected with thep72MT4...
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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
... http://omics.pnl.gov/software/Decon2LS.php). The resulting csv files were further analyzed with viper [40]. A list with a delta m ⁄ z of 4.0247 correspondingto labeled ion pairs with a maximum mass tolerance of10 p.p.m. was generated. ... suberate] with a11.4 A˚spacer arm (Pierce, Waltham, MA, USA). Bothcross-linkers react with the e-amino group of lysine residuesand a-amino group from protein N-termini and, to a lesserextent, with ... 5112–5123 ª 2010 The Authors Journal compilation ª 2010 FEBS 5119A region within the C -terminal domain of Ure2p is shownto interact with the molecular chaperone Ssa1p by the useof cross-linkers and...
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Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc
... were separated on anonreducing SDS/PAGE with (+) or without(–) 20 mMdithiothreitol in the sample buffer.Detection of protein bands was carried out with a His6-antibody and chemiluminescence.Fig. ... cysteineresidues in the N -terminal part of the proteins seems tointerfere with but not abolish the activity. It has beenreported in previous studies that the N-terminally truncatedform of ... evidence for physical interactionsof the N -terminal cysteine pair with the primary reactioncentre. The situation in the C33S mutation appears to bedifferent with respect to the intermediate formed....
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Báo cáo Y học: Differential scanning calorimetric study of myosin subfragment 1 with tryptic cleavage at the N-terminal region of the heavy chain pdf
... with uncleaved S1 and t-S1. Thedecrease in these parameters correlated well with the50kDafi 45 kDa conversion (Fig. 1A). It has been sug-gested from this correlation that tryptic cleavage withinboth ... those S1 molecules, which were cleavedbetween Arg23 and Ile24 in the N -terminal region, but notwithin the 50 kDa segment.N -terminal cleavage dramatically decreasesthe thermal stability of S1The ... mainly through electrostaticinteraction of loop 2 with the negatively charged N -terminal part of actin [38–40]. Thus, the interaction of loop 2 with actin seems to be mainly responsible for actin-inducedstructural...
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Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
... Interaction of EW29Ch with sugarsFEBS Journal 276 (2009) 2095–2105 ª 2009 The Authors Journal compilation ª 2009 FEBS 2105NMR studies on the interaction of sugars with theC -terminal domain of ... barelyinteracts with the region of H1 and the O-methylgroup. The crystal structure of EW29Ch with lactoseshowed that the O2, O3 and O4 atoms of the galac-tose residue of lactose formed hydrogen bonds with EW29Ch, ... interaction with lac-tose than the c sugar-binding site because of the num-ber of intermolecular hydrogen bonds and of residuesinteracting with lactose. Our results agreed well with those from...
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Báo cáo khoa học: C-terminal truncated cannabinoid receptor 1 coexpressed with G protein trimer in Sf9 cells exists in a precoupled state and shows constitutive activity ppt
... fitted with a · 60oil objective, was used to collect the images. CFP wasexcited with a 458 nm laser and images were obtained usinga bandpass filter from 475 to 525 nm. YFP was excited with a ... over glass fibre filters wetted with buffer C.The filters were washed thrice with buffer C. The radio-activity on these filters was counted using a b-countercalibrated with the14C isotope.CoimmunoprecipitationCell ... ul-tracentrifugation and incubated with 50 lL of anti-flag M2IgG agarose (rinsed with buffer B) at 4 °C for 1 h. Thesupernatant was removed and the antibody matrix waswashed three times with buffer B containing...
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Báo cáo khoa học: Peroxin Pex21p interacts with the C-terminal noncatalytic domain of yeast seryl-tRNA synthetase and forms a specific ternary complex with tRNASer potx
... phenotype revealed that C -terminal truncation of SerRS abolished Pex21p binding. Theinteraction is lost upon deletion of the positivelycharged C -terminal fragment (mutant C13) within theextension. ... of a short C -terminal fragmentof SerRS abolishes Pex21p binding withoutaffecting catalytic activityA pull-down assay was used to verify that Pex21p spe-cifically recognizes the C -terminal appendix ... FEBSsaturated with crude E. coli extract containing recom-binant Pex21p, which was immobilized on the resin byits N -terminal His-tag. Ni2+–nitrilotriacetic acidagarose precharged with Pex21p...
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Báo cáo khoa học: Characterization of a digestive carboxypeptidase from the insect pest corn earworm (Helicoverpa armigera) with novel specificity towards C-terminal glutamate residues pptx
... assayed: FAPP, with a C -terminal phenylalanine residue (hydrolysed by carboxypeptidase A);FAAK, with a C -terminal lysine residue (hydrolysed bycarboxypeptidase B) and FAEE, with a C -terminal gluta-mate ... a peptide with aC -terminal neutral, hydrophobic amino acid (Leu) wasnot hydrolysed, like the synthetic substrate FAPP (PheC -terminal residue). Similarly, fibrinopeptide B, with aC -terminal ... complex with carboxypeptidase(s) in vivo.In contrast to the bands eluted at pH 12, the polypeptideseluted by 6Mguanidine hydrochloride had N -terminal sequences with similarity to, or identity with...
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Báo cáo khoa học: The C-terminal region of CHD3/ZFH interacts with the CIDD region of the Ets transcription factor ERM and represses transcription of the human presenilin 1 gene pot
... together with 3 lg of either pCMV-Tag2 orpCMV-Tag2ÆCHD3. pCMV-Tag2ÆCHD3 expresses various N -terminal fragments of CHD3 with the indicated N -terminal end-point (fromposition 1676 –1943). The C -terminal ... times, with a minimum of n ¼ 4 for each data point. Values that differ sig-nificantly from the level of inhibition observed in cotransfections with the C -terminal amino acids 1676 –2000 of CHD3 (1676 ) ... of the C -terminal fragment (amino acids 1676 –2000) with larger fragments including amino acids295–1717, 1005–2000 and 1327–2000. The most signifi-cant activity was observed with the C-terminal...
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Báo cáo khoa học: Crystal structure of the second PDZ domain of SAP97 in complex with a GluR-A C-terminal peptide ppt
... Coimmunoprecipitation of GluR-A CTD with SAP97. HEK293cells were transfected for expression of wild-type (‘wt’) GFP-GluR-A CTD, and mutated CTDs L907A and ‘XA908’ with (A) or without(B) N-terminally myc-tagged ... hydrogen bond with theHis384 at the N -terminal end of aB. These results arein agreement with our previous mutagenesis studywhich indicated that the ability of point-mutatedGluR-A C -terminal domains ... C -terminal residuesexpressed in transgenic mice did not form an immuno-precipitable complex with SAP97 unlike its wild-typecounterpart [10]. In vitro binding of SAP97PDZ1-3toGluR-A C-terminal...
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- an essential metabolite in defence against chemical and oxidative stress in the kinetoplastida the kinetic mechanism for glutathionylspermidine synthetase ec 6318 from crithidia fasciculata cfgsps obeys a rapid equilibrium random terter model with kin
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