... VEGF-R2-positive cells induced from undifferentiated mouse embryonicstem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs), ... suggest that vascular cells derived from human ES cells may have a potential to be a source for therapeutic vascular regeneration after stroke Abbreviations ES cells: Embryonicstem cells; VEGF-R2: ... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis...
... Hematopoieticstemcells for transplantation Nat Immunol 2002, 3: 314- 317 Kaufman DS, Hanson ET, Lewis RL, Auerbach R, Thomson JA: Hematopoietic colony-forming cells derived from humanembryonic stemcells ... derived from human embryonicstemcells exhibit an embryonic gene expression pattern Blood 2004, 103:4134- 4141 Martin CH, Kaufman DS: Synergistic use of adult and embryonicstemcells to study human ... X: CD34+ Cells Derived from Human EmbryonicStemCells Demonstrate HematopoieticStem Cell Potential In Vitro and In Vivo [abstract] Blood 2006, 104:a163 Kaufman DS, Thomson JA: Human ES cells haematopoiesis...
... relief of symptoms Stemcells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stemcells (MSCs) and embryonicstemcells (ESCs) (Lee ... issues of tumorigenicity and functionality of ESCs 2.2 Human EmbryonicStemCells (hESCs) Embryonicstemcells (ESCs) are derived from the inner cell mass of the blastocyst-stage embryos and ... the development of embryonic- like stem cells, also known as induced pluripotent stemcells (iPSCs), as a potential autologous pluripotent stem cell source that can be generated from a patient’s...
... dithiotheritol EBs embryoid bodies ECS Enhanced Chemiluminescent Substrate EG cellsembryonic germ cells ES cellsembryonicstemcells FACS fluorescence-activated cell sorting FAH fumarylacetoacetate ... of human EG cellsfrom aborted fetus has also been reported (Shamblott, Axelman et al 1998) The third type of stem cell is the adult stemcells Like ES and EG cells, adult stemcells can self-renew, ... classification of stemcells What is a stem cell? A stem cell is a cell from the embryo, fetus, or adult that has, under certain conditions, the abilities to self-renew and to give rise to specialized cells...
... Jones JM: Embryonicstem cell lines derived from human blastocysts Science 1998, 282: 1145 - 1147 Fischbach GD, Fischbach RL: Stem cells: science, policy, and ethics J Clin Invest 2004, 114: 1364-1370 ... http://www.tbiomed.com/content/2/1/25 Figure Experimental embryonicstem cell colonies derived from a single blastomere Experimental embryonicstem cell colonies derived from a single blastomere of research in ... Zucker HA: Embryonic death and the creation of human embryonicstemcells J Clin Invest 2004, 114: 1184-1186 Voullaire L, Slater H, Williamson R, Wilton L: Chromosome analysis of blastomeres from human...
... VS, Jones JM Embryonicstem cell lines derived from human blastocysts Science 1998;282(5391): 1145 -7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonicstem cell lines from human blastocysts: ... A An efficient and safe xeno-free cryopreservation method for the storage of human embryonicstemcellsStemCells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes ... feeder layer of mitomycin C-inactivated murine embryonic fibroblast feeder (MEF) cells [7, 8] These were harvested from CF1 inbred mouse strain purchased from Charles River Laboratories Inc (Wilmington,...
... erythroid differentiation of murine embryonicstemcells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonicstemcells at the indicated time points ... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonicstemcells and in human CD 133+ bone marrow cells ... Curr Opin Genet Dev 14, 308–315 20 Kitajima K, Tanaka M, Zheng J, Sakai-Ogawa E & Nakano T (2003) In vitro differentiation of mouse embryonicstemcells to hematopoieticcells on an OP9 stromal...
... ICM PGC TS cells Placental cells 77 ES cells 49 EG cellsEmbryoniccells Fig Epigenetic distances between ES cells and other stemcells derived from developing embryos ES cells derived from the ... profiles Epigenetic study of embryonicstemcells Differences in DNA methylation profiles between ES and other stemcells Comparing ES cells with other stemcells established from developing embryos ... mouse embryonicstemcells Genome Res 14, 1733– 1740 Chen T, Ueda Y, Dodge JE, Wang Z & Li E (2003) Establishment and maintenance of genomic methylation patterns in mouse embryonicstem cells...
... Chapter 33.) Murine EmbryonicStemCells 1 Methods for the Isolation and Maintenance of Murine EmbryonicStemCells Marsha L Roach and John D McNeish Introduction Embryonicstem (ES) cells were rst ... rescuing ES cells during the selection process Murine EmbryonicStemCells 11 Prepare the ES cellsfrom one of the two dishes made d previously for subculture (see Subheading 3.8.3.) While the cells ... develop fromembryonicstem (ES) cells in culture Development 111, 259267 Keller, G., Kennedy, M., Papayannopoulou, T., and Wiles, M V (1993) Hematopoietic commitment during embryonicstem (ES)...
... Development of Hematopoietic Repopulating CellsfromEmbryonicStemCells The In Vitro Differentiation of Mouse EmbryonicStemCells into Neutrophils 10 Development and Analysis of Megakaryocytes from Murine ... from Murine EmbryonicStemCells 11 Development of Lymphoid Lineages EmbryonicStemCells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Differentiation of EmbryonicStemCells 13 ... Culture of Embryonic Germ Cells MARIA P DE MIGUEL AND PETER J DONOVAN 353 Section III Gene Discovery by Manipulation of Mouse EmbryonicStemCells 26 Gene Trap Mutagenesis in EmbryonicStem Cells...
... human [16,17] and mouse embryonicstemcells [18-20] The unique patterns of miRNA expression in embryonicstemcells suggest they are involved in maintaining "stemness" Identifying mRNAs that ... Human EmbryonicStemCells PLoS ONE 2008, 3(7):e2820 Liao R, Sun J, Zhang L, Lou G, Chen M, Zhou D, Chen Z, Zhang S: MicroRNAs play a role in the development of human hematopoieticstemcells ... supervised clustering (A) Adult cells and EB shared genes (B) hES cells upregulated genes (C) EB upregulated genes (D) Adult cells upregulated genes hESC cells EB adult cells Figure supervised hierarchical...
... Figure Hematopoietic differentiation of blast cellsHematopoietic differentiation of blast cells Figures A-F show different types of hematopoietic colonies and cells derived from blast cells A) ... hemangioblasts from human embryonicstemcells Nat Methods 2007, 4(6):501-9 Zambidis ET, Peault B, Park TS, Bunz F, Civin CI: Hematopoietic differentiation of human embryonicstemcells progresses ... molecules on murine embryonicstemcells Biol Reprod 1997, 57(3):561-8 Bonde S, Zavazava N: Immunogenicity and engraftment of mouse embryonicstemcells in allogeneic recipients StemCells 2006, 24(10):2192-201...
... stemcells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stemcellsfrom testis and amniotic fluid stemcells In the book EmbryonicStem ... differentiation of cells derived from human embryonicstemcells Proc Natl Acad Sci U S A, 2000 97(21): p 11307-12 [20] Kroon, E., et al., Pancreatic endoderm derived from human embryonicstemcells generates ... pathways in human embryonicstemcellsStem Cells, 2006 24(6): p 147 6-86 [105] Katoh, M., CER1 is a common target of WNT and NODAL signaling pathways in human embryonicstemcells Int J Mol Med,...
... stemcells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stemcellsfrom testis and amniotic fluid stemcells In the book EmbryonicStem ... differentiation of cells derived from human embryonicstemcells Proc Natl Acad Sci U S A, 2000 97(21): p 11307-12 [20] Kroon, E., et al., Pancreatic endoderm derived from human embryonicstemcells generates ... pathways in human embryonicstemcellsStem Cells, 2006 24(6): p 147 6-86 [105] Katoh, M., CER1 is a common target of WNT and NODAL signaling pathways in human embryonicstemcells Int J Mol Med,...
... Human EmbryonicStemCells 105 Kenji Sakurai, Miho Shimoji, Kazuhiro Aiba and Norio Nakatsuji VI Contents Chapter EmbryonicStem Cells: Introducing Exogenous Regulators into EmbryonicStemCells ... Applications of EmbryonicStemCells in Research and Development 191 Chapter 11 Methods to Generate Chimeric Mice fromEmbryonicStemCells 193 Kun-Hsiung Lee Chapter 12 EmbryonicStemCells in Toxicological ... assessment of retinal pigment epithelium from human embryonicstemcells using transcriptomics Cloning Stem Cells, Vol 6, No 3, pp (217-245) Embryonic StemCells for Therapies – Challenges and Possibilities...
... that employ adult stemcells (such as blood-forming hematopoieticstemcells and cartilage-forming cells) A potential advantage of using adult stemcells is that the patient's own cells could be ... characterization of human embryonicstemcellsStemCells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem- derived cells Trends Biotechnol 2004, 22, 136 -141 19 Evans ... neurons from mouse embryonicstemcells Nat Biotechnol 2000, 18, 675-679 41 Levenberg S, Golub JS, Amit M, Itskovitz-Eldor J, Langer R Endothelial cells derived from human embryonicstem cells...
... of pluripotency in mouse embryonicstemcells Cloning StemCells 2004, 6, 386-391 17 Cobb MH, Goldsmith EJ How MAP kinases are regulated J Biol Chem 1995, 270, 148 43 -148 46 18 Cochrane CG Cellular ... the sodium-nucleoside transport system in brush border membrane vesicles from human kidney Pharm Res 1993, Effect of dihydrotestosterone on mouse embryonicstemcells exposed to H2O2-induced oxidative ... Materials The mouse ES cells were obtained from the American Type Culture Collection (ES-E14TG2a) The fetal bovine serum (FBS) was purchased from Gibco (USA) DHT was acquired from TCI (Tokyo Kasei...
... precursors from human embryonicstemcells Nat Biotechnol 2001, 19:1129-1133 Kaufman DS, Hanson ET, Lewis RL, Auerbach R, Thomson JA: Hematopoietic colony-forming cells derived from human embryonicstem ... VS, Jones JM: Embryonicstem cell lines derived from human blastocysts Science 1998, 282: 1145 - 1147 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A: Embryonicstem cell lines from human blastocysts: ... of DCs from hES cells Derivation of DCs from hES cells: Undifferentiated hES cells were cocultured with S17 mouse stromal cells to derive cystic bodies Later, purified CD34+ cells derived from...
... dopamine neurons from human embryonicstemcells Proc Natl Acad Sci USA 2004, 101:12543-12548 Ying QL, Stavridis M, Griffiths D, Li M, Smith A: Conversion of embryonicstemcells into neuroectodermal ... treated ES cells (Fig 7F) In addition, we also did not find the appearance of cytokeratin 14 (K14) positive cells, representing the epidermal precursor cells, in the SFEB-differentiating ES cells ... Y: Generation of neural crest-derived peripheral neurons and floor plate cellsfrom mouse and primateembryonicstemcells Proc Natl Acad Sci USA 2003, 100:5828-5833 Kohga H, Obata K: Retinoic...