... (2005) Embryonicstem cell differentiation: emergence of a new era in biology and medicine Genes Dev 19 , 11 29 11 55 Jenuwein T & Allis CD (20 01) Translating the histone code Science 293, 10 74 10 80 ... region J Mol Biol 323, 6 01 611 24 Tuan D, Kong S & Hu K (19 92) Transcription of the hypersensitive site HS2 enhancer in erythroid cells Proc Natl Acad Sci USA 89, 11 219 11 223 25 Ling J, Baibakov ... Cell Biol 23, 415 0– 416 1 Scherer CA, Chen J, Nachabeh A, Hopkins N & Ruley HE (19 96) Transcriptional specificity of the pluripotent embryonicstem cell Cell Growth Differ 7, 13 93 14 01 Elefanty AG,...
... G 418 /Ganc/SCML G 418 /SCML HAT/SCML KO-DMEM FBS L-Glutamine MEM/NEAA BME LIF Pen/Strept G 418 Gancyclovir HAT 500 mL 15 0 mL 11 5 mL 11 4 àL 2.5 mL 500 mL 19 0 mL 11 6 mL 11 6 mL 11 4 àL 16 0 àL 11 3 ... mL 19 0 mL 11 6 mL 11 6 mL 11 4 àL 16 0 àL 13 mL 2 .13 .6 mL 11 2 àM 500 mL 90 mL mL mL àL 60 àL mL 2 .13 .6 mL 500 mL 90 mL mL mL àL 60 àL mL mL Store at 4C until used and discard after 14 d 15 17 5300 ... Cycle in ES Cells 29 (9) One of these targets is p21cip1/waf1/sdi1 ES cells not express p21cip1/waf1/sdi1 (2), suggesting that the p21cip1/waf1/sdi1 promoter is not responsive to p53 in ES cells Therefore,...
... Repopulating Cells from EmbryonicStemCells The In Vitro Differentiation of Mouse EmbryonicStemCells into Neutrophils 10 Development and Analysis of Megakaryocytes from MurineEmbryonicStemCells 11 ... CONN VOLUME 14 0 Cumulative Subject Index Volumes 10 2 11 9, 12 1 13 4 VOLUME 14 1 Cellular Regulators (Part B: Calcium and Lipids) Edited by P MICHAEL CONN AND ANTHONY R MEANS VOLUME 14 2 Metabolism ... usually between 1. 5 10 6 and 5 10 5 cells/ dish (3 10 4 to 1 10 4 cells/ cm2) These cell densities should necessitate passaging in 2–4 days with medium being replenished every days A 10 cm diameter...
... miR -18 1b miR-30c miR-26b miR-26a miR -19 0 miR-30e-5p miR- 219 miR-373 miR-363 miR -13 0a miR -14 8a miR-3 01 miR-374 miR1 01 miR-33 miR-25 miR -10 6a miR-524* miR- 517 c miR-520h miR- 517 a miR- 518 c miR- 519 b ... differentiated human embryonicstemcellsStemCells Dev 2007, 16 (6) :10 03 -10 16 Page 15 of 17 (page number not for citation purposes) Journal of Translational Medicine 2009, 7:20 18 19 20 21 22 23 24 25 ... regulation of Pou5f1 8 (10 ) :11 14 -11 23 Zhou Q, Chipperfield H, Melton DA, Wong WH: A gene regulatory network in mouse embryonicstemcells Proc Natl Acad Sci USA 2007, 10 4(42) :16 438 -16 443 Kim J, Chu...
... Møllgård 17 1 Applications of EmbryonicStemCells in Research and Development 19 1 Chapter 11 Methods to Generate Chimeric Mice from EmbryonicStemCells 19 3 Kun-Hsiung Lee Chapter 12 EmbryonicStemCells ... (2005) Efficient differentiation of human embryonicstemcells to definitive endoderm Nat Biotechnol, Vol 23, No 12 , (Dec), pp (15 34 -15 41) 14 EmbryonicStemCells – Basic Biology to Bioengineering ... ES cells in vitro FASEB J, Vol 14 , No 15 , (Dec), pp (2540-2548) 16 EmbryonicStemCells – Basic Biology to Bioengineering Murry, C.E., & Keller, G (2008) Differentiation of embryonicstem cells...
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... 2009, 35:6 51- 657 Zeng et al Journal of Biomedical Science 2 010 , 17 :80 http://www.jbiomedsci.com/content /17 /1/ 80 10 11 12 13 14 15 16 17 18 19 20 Li W, Ma N, Ong LL, Nesselmann C, Klopsch C, Ladilov ... function StemCells 2007, 25: 211 8- 212 7 Wang D, Shen W, Zhang F, Chen M, Chen H, Cao k: Connexin43 promotes survival of mesenchymal stemcells in ischaemic heart Cell Biol Int 2 010 , 12 : 415 -423 Gnecchi ... HO -1- MSCs and Null-MSCs Zeng et al Journal of Biomedical Science 2 010 , 17 :80 http://www.jbiomedsci.com/content /17 /1/ 80 Page of A D DAPI-HO -1- MSCs B HO HO -1 HO -1- MSCs Actin a b E c 555 bp hHO-1...
... cell adhesion model Mol Cell Biol 19 93, 13 :3802-3 810 Jia et al Journal of Biomedical Science 2 011 , 18 :46 http://www.jbiomedsci.com/content /18 /1/ 46 Page 10 of 10 15 Zhang C, Jia P, Jia Y, Weissbach ... Bars: 10 μm Jia et al Journal of Biomedical Science 2 011 , 18 :46 http://www.jbiomedsci.com/content /18 /1/ 46 Page of 10 Figure Confocal microscopy of a single colony of mouse embryonicstemcells ... EST sequence showed 10 0% identity to the truncated cDNA from the 11 3th Jia et al Journal of Biomedical Science 2 011 , 18 :46 http://www.jbiomedsci.com/content /18 /1/ 46 Page of 10 Figure The cDNA...