... inhibits osteoclast formation in vitro J Immunol 20 01, 16 6: 4 915 -49 21 In addition to interleukins that effect osteoclast formation and activity, estrogen and transforming growth factor (TGF) -β ... antiinflammatory roles for IL-23 and IL -12 in joint autoimmune inflammation J Exp Med 2003, 19 8 :19 51- 1957 Nakae S, Nambu A, Sudo K, Iwakura Y: Suppression of immune induction of collagen-induced ... formation and when administered together at synergistically low doses both GM-CSF and IFN-γ are produced [8] Leptin has both central and peripheral roles in influencing bone mass, with leptin in the...
... can be protected against influenza A infection by vaccination with vacciniainfluenza recombinants expressing hemagglutinin and neuraminidase J Immunol 19 93, 15 0 (12 ):5484-5493 Bender BS, Bell WE, ... Purification kits The DNA was banded twice on CsCl2 gradients Vaccinia viral gene recombinants Thymidine kinase-negative (TK-) recombinant vaccinia virus expressing influenza A/PR/8/34 NP gene (NP-Vac) ... present findings is that NP-specific CD8+ CTL activity can augment protection against influenza induced by antibody and is a desirable immune response for influenza vaccines Competing interests...
... (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein Proc Natl Acad Sci USA 94, 59 61 5 966 11 Zhou J, Tang X & Martin GB (19 97) The Pto kinase conferring ... to GCC and DRE motif-containing sequences Four ERF domains were tested for binding to the 16 bp DRE or GCC motif-containing sequences using quantitative EMSA, as described in Materials and methods ... leading to the establishment of the selected binding site Phylogenetic analysis Binding competition assay The binding condition and buffers used in the competition assay were the same as used in...
... Replication protein A is the major single-stranded DNA binding protein detected in mammalian cell extracts by gel retardation assays and UV cross-linking of long and short single-stranded DNA molecules ... previously demonstrated to bind to GT in a nonspecific manner [12 ] Characterization of eEF1A in normal and cancer cells In order to compare the binding properties of the nuclear eEF1A in normal cells ... lines J Natl Cancer Inst 83, 757– 766 19 Bradford, M.M (19 76) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding...
... Fig Itk in uences the balance of conventional and innate T-cell lineages In the absence of Itk, there is a reduction of conventional CD4+ and CD8+ cells and an expansion of innate CD4+ and CD8+ ... Clin Med Res 4, 14 7 15 1 Smith-Garvin JE, Koretzky GA & Jordan MS (2009) T cell activation Annu Rev Immunol 27, 5 91 61 9 , doi :10 .11 46/ annurev.immunol.0 219 08 .13 27 06 10 .11 46/ annurev.immunol.0 219 08 .13 27 06 ... ligand expression and activation-induced cell death in the absence of IL-2-inducible T cell kinase J Immunol 16 8, 2 16 3– 217 2 40 Qi Q, Kannan AK & August A (2 011 ) Tec family kinases: Itk signaling...
... alternating splicing at the interface between exon and exon 3, leading to insertion of an additional exon, termed exon 2B, in BIR domain Since BIR domain is a functional domain that is important ... breast cancer cells Cancer Res 2005, 65 :11 018 -11 025 Page 10 of 11 (page number not for citation purposes) Journal of Translational Medicine 2009, 7 :1 10 11 12 13 14 15 16 17 18 19 20 21 22 23 ... differences in the staining intensity among the cases, we couldn't find any correlation between the staining intensity and the CTL induction efficiency Why does survivin have so immunogenic feature...
... 59 60 61 62 63 64 65 66 67 68 69 70 lated kinase (ERK) and Jun NH2-terminal kinase (JNK) activation, but does not affect phosphorylation of T cell receptor zeta and ZAP70 J Exp Med 19 97, 18 6 : 16 45 - 16 53 ... have overlapping functions [5], despite different binding determinants, different dissociation kinetics, and different binding affinities for CD28 and CTLA-4 Differences in their functions seem to ... heavy-chain constant region of human IgG1 CTLA-4Ig binds CD80 and CD 86 on APCs, interfering with B7/CTLA-4 and B7/CD28 ligation In collagen-induced arthritis in rats, CTLA4Ig has prevented disease induction...
... influences dynamic evolution and pathogenic functions of HIV -1 Nef J Immunol 2008, 18 0 :11 07 -11 16 Ueno T, Idegami Y, Motozono C, Oka S, Takiguchi M: Altering effects of antigenic variations in ... between viral adaptation and immune recognition during HIV -1 infection Protein & Cell 2 010 , 1: 514 - 519 Brockman MA, Schneidewind A, Lahaie M, Schmidt A, Miura T, DeSouza I, Ryvkin F, Derdeyn CA, ... naturally-arising CTL escape variants in the wellconserved PxxP region in Nef alone or in combination can modulate some pathogenic functions of Nef in the Mwimanzi et al Retrovirology 2 011 , 8:50 http://www.retrovirology.com/content/8 /1/ 50...
... validate a balanced transcription between telomerase and the telomeric DNA-binding proteins TRF1, TRF2 and POT1 in normal, activated as well as in HTLV -1 infected andin Tax-expressing T lymphocytes ... expression in HTLV -1- infected and uninfected T cells Real time quantitative PCR (qPCR) analysis of hTERT expression in HTLV -1- infected and uninfected T cells A) ATL cell lines (HTLV -1 infected but negative ... negatively regulated in vivo, at the level of telomere itself, by several shelterin subunits, including TRF1, TIN2, TPP1, Pot1 and Rap1 For instance, Pot1, a single-stranded telomeric DNA-binding...
... 20 06, 3 :1 http://www.retrovirology.com/content/3 /1/ 1 A Plasma SIV RNA (copies log10/ml) 3 10 14 17 21 24 28 31 35 38 42 56 70 83 96 14 6 15 2 16 7 19 3 209 83 96 14 6 15 2 16 7 19 3 209 Day post-infection ... critical for function and be constrained such that any change in epitope sequence results in a significant deficit in the replicative fitness of the virus Thus, in an operational definition, an "immutable" ... RT-PCR, cDNAs were cloned into a plasmid Individual clones were then isolated and sequenced In parentheses, the numerator indicates the number of clones with a given sequence and the denominator...
... in order to reduce the system resource used in T-Engine The training phase is implement in a PC with speech data from T-Engine, only the recognition module is implemented in T-Engine IV VIETNAMESE ... for n = 0 ,1 L -N/ 2 -1 s [n] = x [n] + p.y [n- L +N/ 2] for n = L -N/ 2 L -N/ 2+K -1 s [n] = p*y[L -N/ 2+k] for n = L -N/ 2+K L+K; To reduce memory needed in implementation of the above algorithm for T-Engine embedded ... combined speech recognition together with speech synthesis into our software running in T-Engine This software allow users to use speech-commands to query information about places in Ha Noi Figure...
... mutant HFENNN 110 /13 0/234–HA (triple) or single mutants HFE -N 110 A–HA, HFE -N1 30A–HA and HFE -N2 34A–HA or (C) double mutants HFE-NN 110 /13 0AA–HA, HFE-NN234 /11 0A– HA or HFE-NN234 /11 0AA–HA, as indicated ... and an association with binding immunoglobulin protein J Biol Chem 266 , 17 64 8 17 65 4 Williams A & Enns C (19 93) A region of the C-terminal portion of the human transferrin receptor contains an ... HFEWT–HA, HFE-NN 110 /13 0AA–HA, HFE-NN130/234AA–HA or HFENN234 /11 0AA–HA protein were incubated for 16 h in the presence or absence of mM tunicamycin as indicated (Tunica) HA-tagged proteins in cleared...
... protein bands in Jurkat cells (C) Linear depiction of Ca1, Cb1 and Cb2 amino acid sequence and the localization of the domains ⁄ antigens used to generate the different anti-C antibodies used in ... (Fig 1C and Experimental procedures) AntiCb2(SNO103) recognized the 47 kDa protein band in T cells and Jurkat cells, while anti-Cb(SNO157) recognized a 47 kDa band in T cells and Jurkat cells and ... immunoblotted using the anti-Cb2(SNO103) antibody, as shown in Fig 2A (middle panel) The anti-Cb2 antibody recognized a 47 kDa band only present in the Cb2-transfected cells and not in the Ca1...
... Biol 16 , 3934±3944 Sangfelt, O., Erickson, S., Einhorn, S & Grander, D (19 97) Induction of Cip/Kip and Ink4 cyclin dependent kinase inhibitors by interferon-alpha in hematopoietic cell lines Oncogene ... using Annexin V FLUOS and Propidium Iodide staining kit according to the manufacturer's protocol (Boehringer Mannheim, Mannheim Germany) Quantitation of band intensity from Firpo et al [18 ] and ... Dier 10 , 575±582 17 Erickson, S., Sangfelt, O., Heyman, M., Castro, J., Einhorn, S & Grander, D (19 98) Involvement of the Ink4 proteins p 16 and p15 in T-lymphocyte senescence Oncogene 17 , 595 60 2...