1 1 self renewal and pluripotency
Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 2
... Mesendoderm Endoderm 16 00 14 00 12 00 RNAi1 10 00 RNAi3 Control 800 600 400 200 Furin Pdgfr Nr2f2 Gata6 Gata4 Sox17 Foxa2 Cer1 Sox7 Fgf8 Nodal Gsc Mixl1 T Lefty1 Pitx2 Bmp4 Flk -1 Nkx2.5 Ngn1 Pax6 Nestin ... (Pur- puromycin) 45 2 .1. 2 Results 2 .1. 2 .1 Identification of Lefty2 as a candidate for self renewal and pluripotency from a high throughput RNAi screen To ensure reliability and specificity of the ... Sox7 Hex Pthr1 FoxH1 E-cadherin Foxa2 Gsc Nodal Cad 11 Claudin6 Mixl1 Pitx2 T Flk -1 Lefty1 Lefty2 Figure 10 a Lefty2 knockdown using RNAi3 in the absence of LIF led to differentiation of E14 ES cell...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 3
... maintenance of ES cell self- renewal To achieve this aim, a candidate gene approach was adopted and 70 candidates were shortlisted An RNAi screen in mouse ES cells (E14) using Oct4 and Nanog promoter ... understanding of ES cell self renewal mechanism beyond the established roles of LIF/STAT3, BMP and WNT pathways and that of the core transcriptional regulatory circuitry governed by OCT4, SOX2 and ... inhibitor SB4 315 42 and Lefty protein supplementation induces trophectodermal differentiation This suggests that Nodal/Activin signaling must be tightly regulated to sustain ES cell self renewal, as...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 4
... IN 1/ 500 1/ 500 1/ 1000 Rabbit Rabbit Rabbit 9 514 S 1/ 1000 Rabbit SC-6200 1/ 200 Goat SC-7966 1/ 1000 Mouse ab57 31 SC -16 16 1/ 1000 1/ 2500 Rabbit Goat SC-8628 1/ 2000 Goat AM392-5M 1/ 200 Mouse MS -17 41- S0 ... NUMBER probes, A 110 17 1/ 400 RAISED IN Goat Molecular Invitrogen probes, A 110 72 1/ 400 Goat Molecular Invitrogen probes, A 110 55 1/ 400 Donkey Molecular Invitrogen probes, D- 214 90 1/ 1000 - Molecular ... (I -19 ) CDX2 (clone 88) P-Cadherin AB1 (Clone56C1) COMPANY CATALOGUE NUMBER Chemicon ab57 31 Abcam ab15580 Santa Cruz SC -16 16 Biotechnology BioGenex AM392-5M DILUTION RAISED IN 1/ 1000 1/ 1000 1/ 2500...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 5
... roles and regulation Development 13 4, 10 23 -10 34 Shi, W., Wang, H., Pan, G., Geng, Y., Guo, Y., and Pei, D (2006) Regulation of the pluripotency marker Rex -1 by Nanog and Sox2 J Biol Chem 2 81, 23 319 -23325 ... Chem 276, 213 97- 214 04 19 2 Vackova, I., Ungrova, A., and Lopes, F (2007) Putative embryonic stem cell lines from pig embryos J Reprod Dev 53, 11 37 -11 49 Vallier, L., Alexander, M., and Pedersen, ... Sci 12 0, 55-65 Oh, S P., and Li, E (19 97) The signaling pathway mediated by the type IIB activin receptor controls axial patterning and lateral asymmetry in the mouse Genes Dev 11 , 18 12 -18 26...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
... the murine system in 19 81 by Martin Evans and Matthew Kaufman and independently by Gail R Martin (Evans and Kaufman, 19 81; Martin, 19 81) Subsequently, successful derivation and propagation of rodent, ... pathways and mechanisms that maintain pluripotency and ES cell self renewal may exist 1. 2 .1. 2 Bone morphogenetic factor and BMP signaling In ES cell media without LIF, there is limited self renewal ... important for sustaining ES cell self renewal 1. 2.2 Intrinsic regulators and mechanism governing ES cell self renewal 1. 2.2 .1 Oct4 Oct4 (also known as Oct3 or Pou5f1) is a POU (Pit, Oct, Unc) family...
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Appendix identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
... 84.4 10 2.2 11 8.0 11 7 .1 229.7 10 3.2 205.0 2 41. 9 12 1 .1 182.5 17 9.4 12 2.5 326.8 339.7 14 3 .1 134.7 208.9 308.3 816 .9 8 01. 3 242.5 997.6 756.6 315 .6 582.9 215 .3 16 2 .1 256.2 17 9.6 246.4 568.7 467.7 4 61. 7 ... 65.3 10 7.8 10 1.6 30.3 10 8.8 29.6 33.3 57 .1 73.5 55.7 83 .1 16.8 23.0 78.4 16 9.4 10 8.5 56.3 11 2.8 90.8 10 6.2 7.8 4.2 11 4.4 15 9.8 13 .8 12 7.5 0.2 0.5 62.3 64 .1 159.8 96.4 12 3.7 10 3.0 16 4.0 267.6 31 84.4 ... 50.8 16 4.4 51. 4 44.4 87.0 11 8.3 44.3 77.9 12 2.3 95.2 68.8 56.5 35.5 73.6 12 7.3 14 1.6 10 9.2 94.7 52.7 84.8 10 7.3 84.0 88.3 15 8.6 15 7.6 262 .1 215 .8 999.5 12 85.7 392.6 640.8 762.2 12 91. 2 226 .1 117 .7...
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Báo cáo y học: "Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation" potx
... 2,82,86-88,90 10 0 2,82,84- 91 CD34 1 10 2 ,12 ,63,82,84-89, 91 CD45 0 11 0 2,82,84- 91 CD 117 1 13 2,63,82,87-90 Sca -1 0 63,87-88,90 CD10 82,85-87,89 CD90 11 1 10 2 ,12 ,63,82,84-85,87- 91 Flk -1 Variable CD11b ... Stro -1 0 4-7,66,82-84 0 1 2 ,12 ,84-87,89-90 CD29 0 11 0 2 ,12 ,63,84-87,90 CD44 11 10 2,63,82,84-87,90- 91 CD73 0 0 2 ,10 ,83-85 CD105 Positive Surface antigena CD13 Marker type 0 0 2 ,10 ,12 ,83-87 CD106 ... http://arthritis-research.com/content/9 /1/ 204 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Dennis JE, Carbillet JP, Caplan AI, Charbord P: The STRO -1+ marrow cell population is multipotential Cells Tissues Organs 2002, 17 0:73-82...
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Báo cáo khoa học: Modulation of glucocorticoid receptor-interacting protein 1 (GRIP1) transactivation and co-activation activities through its C-terminal repression and self-association domains pptx
... 13 05 13 98 14 62 14 62 14 62 13 98 11 22 10 13 II 13 05 11 22 13 05 I 13 98 + 14 62 11 22 13 05 10 13 + 13 98 13 05 11 22 13 98 14 62 14 62 10 13 11 22 13 05 13 98 14 62 + 14 62 13 98 11 22 11 22 10 13 13 05 62 13 98 14 62 13 05 ... GST 11 22 -13 04 13 05 -13 98 13 05 -14 62 10 13 99 -14 62 11 22 -14 62 GST-GRIP1 GST GST-GRIP1 Input 10 % B GRIP1 11 22 -14 62 GST-GRIP 113 05 -13 98 GRIP1 GST Input 10 % C 14 62 765 563 11 21 112 2 14 62 Fig GRIP1 forms ... 10 x AD1 11 22 Luciferase Activity B (RLU 10 ) 12 0 [Gal4DBD; pM vector] 563 11 21 AD1 10 13 AD1 Mr 14 62 AD2 10 16 0 1x 10 13 11 21 AD1 C 40 1x [Gal4DBD; pM vector] 35 858x 29000x Mr 10 55 40 10 0 72...
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Clinical Vignettes for the USMLE Step 1 PreTestTM Self-Assessment and Review Fourth Edition pot
... 11 3 Block 13 7 Answers Block 16 1 Block 18 5 Block ... = 14 mEq/L pH = 7.40; PaCO2 = 20 mmHg, [HCO3–] = 10 mEq/L, Anion Gap = 26 mEq/L 1- 11 A 63-year-old man presents with signs of congestive heart failure, including shortness of breath, cough, and ... cell DNA and hybridization with allele-specific oligonucleotides (PCR-ASOs) e Southern blot analysis for gene deletions 1- 31 An 11 -year-old boy presents with ciliary dyskinesia, sinusitis, and bronchiectasis...
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Period 1 SIMPLE PRESENT AND PRESENT PROGRESSIVE TENSES.doc
... ………………………………… 10 They never (go) fishing in winter ……………………………………………………… 11 She (go) to school now ……… ……………………………………………… 12 The students (not be) in class at present ……… ……………………………………………… 13 The ... ……………………………………………… 14 Water (boil) 10 00C It (boil) now ……… ……………………………………………… 15 Be careful! The bus (come) ……… ……………………………………………… 16 Listen! Someone (knock) the door ……… ……………………………………………… 17 Look! ... of) 3.He was busy yesterday and …… was I (too/ either/ so/ neither) What does she look like? - She’s …… (interesting and sense of humor/ generous and helpful/ tall and thin) I received a letter...
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Exergoeconomic performance optimization of an endoreversible intercooled regenerated Brayton cogeneration plant Part 1: Thermodynamic model and parameter analyses
... J., 19 96, 21( 12): 11 27 -11 34 [36] Chen L, Sun F, Chen W Finite time exergoeconomic performance bound and optimization criteria for two-heat-reservoir refrigerators Chin Sci Bull., 19 91, 36(2): 15 6 -15 7 ... calculations, k = 1. 4 , Cwf = 1. 0kW / K , τ = τ = and τ = 1. 2 are set According to analysis in Ref [46], a = 10 and b = are set 4 .1 The total pressure ratio is fixed Assuming that π = 18 (1 < π ≤ 18 ) The ... reserved 210 International Journal of Energy and Environment (IJEE), Volume 2, Issue 2, 2 011 , pp .19 9- 210 Lingen Chen received all his degrees (BS, 19 83; MS, 19 86, PhD, 19 98) in power engineering and...
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Lab 5.1.6 Hub and NIC Purchase
... show the choices and the features or factors that were compared, such as number of ports, features, price, performance, and so on 2-2 CCNA 1: Networking Basics v 3.0 - Lab 5 .1. 6 Copyright 2003,...
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Lab 5.1.7 Hub and NIC Purchase
... show the choices and the features or factors that were compared, such as number of ports, features, price, performance, and so on 2-2 CCNA 1: Networking Basics v 3.0 - Lab 5 .1. 7 Copyright 2003,...
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unit 8 lesson 1: getting started and listen and read
... Lien village b) She was there for two day c) Her opinion of the countryside is it’s quiet and peaceful and there is nothing to d) May be she means that the countryside lack of facilities such ... _Entertainment (n) vui chơi giải trí _Medical (adj) thuộc y tế II) Answer the question: 1) Question a) Where has Na been? b) How long was she there? c) What is her opinion of the countryside? ... electricity f) I Liked the city Because, there are tall buildings, kinds of goods, amusement parks and no sorrow ...
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Unit 7 Lesson 1 Getting...Listen and read
... -to turn off faucets after use START 41 42 31 08 30 29 28 27 26 25 20 24 23 13 14 21 22 12 04 03 02 10 09 19 17 11 15 18 16 07 06 05 00 01 39 49 34 32 51 40 54 48 44 45 52 53 43 47 38 36 35 33 ... energy at home and at schooll ?” “ What you to save energy at home and at schoo ?” Period 43 Saturday , December 13 th 2008 Unit : SAVING ENERGY Lesson Getting started Listen and read – P.57 ... Saturday , December 13 , 2008 Period 43 Unit saving energy Lesson Getting started Listen and read – P.57 I Vocabulary: A water bill Hoá đơn tiền nước Period 43 Saturday , December 13 th, 2008 Unit...
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L9 Unit 7 Lesson 1 Getting...Listen and read
... -to turn off faucets after use START 41 42 31 08 30 29 28 27 26 25 20 24 23 13 14 21 22 12 04 03 02 10 09 19 17 11 15 18 16 07 06 05 00 01 39 49 34 32 51 40 54 48 44 45 52 53 43 47 38 36 35 33 ... energy at home and at schooll ?” “ What you to save energy at home and at schoo ?” Period 43 Saturday , December 13 th 2008 Unit : SAVING ENERGY Lesson Getting started Listen and read – P.57 ... Saturday , December 13 , 2008 Period 43 Unit saving energy Lesson Getting started Listen and read – P.57 I Vocabulary: A water bill Hoá đơn tiền nước Period 43 Saturday , December 13 th, 2008 Unit...
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Bài giảng Chapter 1 Matter,Measurement, and Problem Solvin
... Hill 11 Do we need science? A history lesson in science • A key feature of science are it’s experiments • Experiments must be duplicated by other’s!!! • Galileo (15 64 - 16 42) and Newton (16 42 17 27) ... Subliming Dry Ice C12H22O 11( s) Dry Ice boiling / condensing melting / freezing subliming • dissolving Chemistry, Julia Burdge, 2nd e., McGraw Hill CO2(g) CO2(s) C12H22O 11( aq) 46 Common Chemical ... Chemistry • Curiosity and your imagination are your allies Explore and investigate • Quantify and calculate Even small differences can be important! • Commitment Work regularly and carefully Chemistry,...
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Chapter 1 - The role and environment of managerial finance
... Finance and Business Strengths of the basic legal forms of business organisation Table 1. 1, page Copyright © 2 011 Pearson Australia (a division of Pearson Australia Group Pty Ltd) – 97 814 42 518 193/ ... Finance and Business Weaknesses of the basic legal forms of business organisation Table 1. 1, page Copyright © 2 011 Pearson Australia (a division of Pearson Australia Group Pty Ltd) – 97 814 42 518 193/ ... 2 011 Pearson Australia (a division of Pearson Australia Group Pty Ltd) – 97 814 42 518 193/ Gitman et al / Principles of Managerial Finance / 6th edition Finance and Business Finance: the art and...
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Tài liệu Check Point™ NG VPN-1/FireWall-1 Advanced Configuration and Troubleshooting pptx
... VPNs 12 1 12 2 12 3 12 5 12 6 12 6 12 8 12 9 13 1 13 1 13 1 13 2 13 5 13 5 14 0 14 2 14 6 14 6 14 7 14 7 15 0 15 1 15 3 15 3 15 5 15 7 15 8 15 8 15 9 16 0 16 1 16 3 16 5 16 6 17 4 18 1 18 2 259_ChkPt_VPN_TOC.qxd 4/4/03 12 :14 PM ... New Mode xix 18 5 18 5 18 8 19 1 19 2 19 2 19 2 19 2 19 3 19 3 19 3 19 3 19 3 19 3 19 4 19 4 19 4 19 5 19 6 19 8 19 8 19 9 19 9 200 200 2 01 2 01 202 202 207 208 208 209 224 259_ChkPt_VPN_TOC.qxd xx 4/4/03 12 :14 PM Page ... User Management Self- Service User Management with ADSI xvii 57 58 61 62 62 63 64 69 72 73 76 79 81 82 83 89 90 92 94 95 96 97 99 10 2 10 4 10 5 10 6 10 8 10 9 11 0 11 1 11 2 11 4 11 4 11 7 259_ChkPt_VPN_TOC.qxd...
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Tài liệu Lab 3.1.1 Safe Handling and Use of a Multimeter docx
... voltage is negative when measuring a battery, what is wrong? 2-2 CCNA 1: Networking Basics v 3.0 - Lab 3 .1. 1 Copyright 2003, Cisco Systems, Inc ... taken to avoid electrical shock Step Insert the red and black leads into the proper jacks on the meter a The black probe should go in the COM jack and the red probe should go in the + (plus) jack ... Step Swith or turn to different measurements For example, voltage, and ohms a How many different switch positions does the multimeter have? b What are...
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