03 design modeling microfabrication and characterization of the micro gas chromatography columns
Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells
... polymerization of styrene and the blending of PS and SPS polymers have affected the surface structure of the PVDF membrane obviously The cross-section morphologies of the PVDF membrane, the PVDF-PS ... Thermal analysis of the PVDF-SPS composite membrane The TG analysis curve is presented in Figure for the investigation of the thermal stability of the PVDF membrane, PVDF-PS membrane, and the PVDF-SPS ... to prepare the PVDF composite membrane The structure and the morphology of the PVDF-SPS composite membrane were characterized The proton conductivity and the methanol permeability of the PVDF-SPS...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... important in the generation of the correct class of antibodies by B-cells, and for the elimination of extracellular pathogens, such as helminths and other extracellular parasites [2] Th17 is the most ... domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3 In addition, for stat6 and foxp3, there were a few other conserved features Within the ... tissue of mouse and spleen and thymus of human and mouse [11,37] Foxp3 is also highly expressed in lymphoid organs, such as the thymus and spleen [49], and its expression within a variety of other...
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Tài liệu Báo cáo khóa học: Non-specific depolymerization of chitosan by pronase and characterization of the resultant products pptx
... cm2 per residue using the mean residual weight of GlcNAc [24] Further characterization of chitosan and the depolymerization products Further characterization of chitosan and the depolymerization ... comparison of band absorbance Absence of a sharp and convoluted spectral band around 3600–3000 cm)1 in the spectra of both chitosan and LMWC indicated the absence of free -OH groups and the involvement ... (moles/L) · volume of titrant (mL)/mass of sample (g) and the level of deacetylation was calculated by comparison between the number of free amino groups (per unit weight of the sample) and the equivalent...
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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
... shown in the inset, the progressive decrease of the molecular ellipticity at k ¼ 200 nm (the absorption region of the peptide bond) reflects the occurrence of a transition between 35° and 55 °C ... one PHR and one PHO (abc) protomer The hypothesis of a direct PHI–phenol interaction is quite unlikely, because of the fact that the addition of phenol does not alter the emission spectrum of PHI ... identity between the N-terminal sequence of PHI and those of ferredoxin-like proteins belonging to other oxygenases On the contrary, the N-terminus of PHI is identical to that of MopN from A calcoaceticus...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... GroEL from 15 and 30 lg of the cell lysate without (lanes and 3) or in the presence of ATP (lanes and 4) was detected with anti-UP12 Ig Samples (3 and lg of total proteins) of the cell extract ... UP12 and UspA is not very pronounced, the following observations support the classification of UP12 as a member of the UspA family Sub-cloning, purification, and characterization of UP12 and its ... anti-GroEL Ig [27] The high yield of the UP12-GroEL complex isolation and the ATP-mediated dissociation of the complex strongly support the suggestion that the two stress proteins GroEL and UP12 interact...
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Báo cáo khoa học: Purification and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt
... protein content in their latex; and (2) the presence of other cysteine proteinases or isoforms in the latex To investigate these two hypotheses, the protein concentration per milligram of dried latex, ... tapped for the first time, we are not aware of the frequency of tapping in other studies These and probably several other, as yet unknown, factors affecting the activity and concentration of latex ... sequences of the proteins VXH-I, VXH-III, VXH-IVa and VXH-IVb are shown in Fig 6, and are compared with the known N-terminal sequences of the cysteine proteinases of C papaya and V cundinamarcensis The...
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Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot
... account the possible size of the mlp gene family and the number of gene variants considered in the construction of the M1 and M2 primers, the error may be not large In our estimate, one copy of the ... role of the sequence (Fig 2) The sequences of the catalytic A-chain of the MLp toxins were found to differ However, the amino acids forming the catalytically active center of the A-chains of the ... (and BclI, data not shown) digests gave a very similar pattern of five bands, one of which was also more intensive than the others Thus, the different intensity of the bands may be the result of...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... 205 and 215 nm, indicating that Rv1399c is composed of 22% of a-helices, 25% of b-strand and 39% of random coil The catalytic triad is located at the bottom of a small ˚ ˚ pocket, 20 A length and ... experimentally Indeed, the presence of Arg213 along with the proximity of the charge relay Asp260 within the active site could be responsible for the modification of the catalytic properties of the catalytic ... by the amides of Ala163, Gly89 and Gly90, the latter’s being found in the HGGG consensus sequence motif characteristic of members of the HSL family [35] Our model shows that the architecture of...
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Báo cáo Y học: Cloning, chromosomal localization and characterization of the murine mucin gene orthologous to human MUC4 pdf
... experiments The last exon is composed of a coding sequence of 193 bp and of an untranslated region of at least 131 bp The size of the 24 introns ranges from 79 bp to about 16 kb The size of the largest ... fragments of 1.6 and kb named 2350 and 2355, respectively They both overlap the 3¢ end of the repeat region To sequence the 5¢ region, we performed RT-PCR using NAU972 (designed from SMC) and the antisense ... using random hexamers or oligo(dT) primer Several oligonucleotides (Fig 1) were designed by comparison of the similar regions in the 3¢ regions of the gene coding for the rat SMC and of the human...
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Báo cáo khoa học: Bovine tryptases cDNA cloning, tissue specific expression and characterization of the lung isoform ppt
... other species, except in the case of sheep tryptases and [13], where the identity reaches 82–83% The major and more significant difference between BLT and BLCT resides at positions 188–189 of the ... Furthermore, analysis of BLCT and BLT expression in bovine heart and spleen has shown that both enzymes are present in these tissues at the mRNA level The simultaneous expression of the two isoforms ... agarose gel electrophoresis and the bands eluted, purified and subcloned in the PCRIITM TOPO vector containing the lac promoter and the b-galactosidase gene, using the TA Cloning Kit (Invitrogen,...
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Báo cáo khoa học: Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme pdf
... Glycosylation was 0.5% of the molecular mass of the native laccase and and 10% for the recombinant laccase In the heterologous production of the P cinnabarinus laccase in P pastoris [14] or the T villosa ... performed to determine the isoelectric point The protein was, as the wild-type, very acidic and the pI estimated to be 3.7 Effect of temperature and pH on laccase activity Studies of the recombinant ... Effect of the temperature on the activity of the purified laccase Various temperatures in the range of 25 °C to 85 °C were tested with 500 lM ABTS as the substrate 20 kDa Fig SDS/PAGE gel analysis of...
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Báo cáo khoa học: Expression and characterization of the biofilm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase from Vibrio alginolyticus pot
... downstream from His80 in the vicinity of the zinc center and is assumed to clamp the imidazolium ring of His80 Glu149 is in the immediate vicinity of the Glu150 of the zinc center and is assumed to ... examined the V alginolyticus PepD protein In the present study, we present the cloning and expression of the V alginolyticus pepD gene, the purification and biochemical characterization of the produced ... through the activation of the water molecule by both the zinc ions and transfer of the proton to the Glu149; (d) the carbonyl oxygen will be bound in an ‘oxyanionbinding hole’ formed by Zn1 and the...
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Báo cáo khoa học: Heterologous expression of a serine carboxypeptidase-like acyltransferase and characterization of the kinetic mechanism potx
... forward primers Design and synthesis of the AtSMT sequence adapted to the codon usage of S cerevisiae was performed by GENEART Construction of the multicopy-plasmid pDIONYSOS The leu2-d marker ... infected with mL of the virus stock After approximately one-third of the cells were lyzed (72 h of incubation), they were harvested and pelleted The cells were resuspended in 1.5 mL of buffer (100 ... codon usage of the Arabidopsis gene as well as the nature of the signal peptide and the sequence motif around the translation start as critical parameters for efficient expression of AtSMT in...
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Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot
... dsRNA They suggest that the complete silencing of the gene transcript could not be achieved due to dilution of dsRNA in the feeding stage of the female tick [31] On the other hand, Acosta and coworkers ... observed the amplification by RT-PCR of a defined band of 260 bp in the saline treated group only These results suggest the possible complete degradation of the CHH transcript because of dsRNA ... Pharmacia) according to the instructions of the manufacturer Northern blot analysis Northern blot analysis was used to characterize the expression of the shrimp CHH gene Ten micrograms of each RNA sample...
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Báo cáo Y học: Cloning and characterization of the mammalian-specific nicolin 1 gene (NICN1) encoding a nuclear 24 kDa protein doc
... from the experiments shown in Figs and In each experiment the tissue with the highest expression level was set to 100 The height of the other bars indicates the relative amount of NICN1 mRNA in the ... dependent on the metabolic state of the organ As the RNA for the Northern blot and the RT-PCR analysis had been derived from two different anonymous human donors the differences in the observed ... enzyme of glycine metabolism [4,11] The promoters of the canine and murine NICN1 genes lack a TATA box-motif In both species, the sequences in the promoter regions fulfill the criteria of CpG islands...
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Báo cáo Y học: Puri®cation and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli doc
... Gly-Ser, in-frame with the codon for Pro2 of the receptor cDNA The codon for the last used amino acid of the receptor (Ser412 in case of the full-length receptor and Ala316 for the truncated receptor) ... concentrations of 10±20 times their Ki value Without ligand, a half-life of 33 days was obtained This increased to 77 days in the presence of NECA (agonist) and 53 days in the presence of theophylline ... temperature and using high concentrations of the weak antagonist theophylline combined with low ¯ow rates The ®nal ionexchange step allowed removal of theophylline, and a reduction of volume and detergent...
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Báo cáo khoa học: Identification and characterization of the metal ion-dependent L-alanoyl-D-glutamate peptidase encoded by bacteriophage T5 pdf
... bacteriophage T5+ were taken from the collection of the Laboratory of Molecular Microbiology of the Institute of Biochemistry and Physiology of Microorganisms (IBPM RAS) Strains of E coli K12, B subtilis, ... aspartic acid of the enzyme and an acidic N-terminal amino acid of the substrate [26] The affinity of bacteriophage T5 endolysin for d-glutamic acid of peptidoglycan and the ability of endolysin ... Asp73, Asp130, and His133) that are typical for metallopeptidases of the M15 family and take part in the binding of the metal ion in the process of catalysis [28] This is the first example of an l-alanoyl-d-glutamate...
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Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt
... of degenerate and specific primers and by rescreening the cDNA libraries The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails ... representation of the full length XHIVEP1 and the Myc-tagged (MT) deletion mutants Black and white boxes indicate the position of the Znf domains and the serine-rich stripe, respectively The relative ... bold The position of the terminal amino acid for the depicted sequence is indicated to the right (Fig 6B, compare lanes and with and 6) Mutation of the GAGA motif only slightly altered the ZAS-N...
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Báo cáo khoa học: Coexpression, purification and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli potx
... start of the oraS gene and a BamHI site into the end of the oraE gene Genomic DNA was purified from C sticklandii by phenol/chloroform extraction methods [4] The coding regions for the S and E ... determined by measuring the apparent Km value of D-ornithine at 25, 50, 100, 200 and 400 lM of the inhibitor For the measurement of the activity of the substrate analogues, an HPLC and NMR-based method ... copies of the argU, ileY, and leuW tRNA genes The coprecipitation of OraS and OraE might imply that (a) the apoenzyme or OraE is not properly folded; and (b) the noncovalent interaction between these...
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Báo cáo khoa học: Expression, purification and characterization of the second Kunitz-type protease inhibitor domain of the human WFIKKN protein pot
... [8–10]) Thermal unfolding of the protein was monitored at 203 nm at a heating rate of 60 °CÆh)1 Effect of the recombinant protein on the activity of proteases The activity of the proteases on synthetic ... (JASCO) Fig Alignment of the sequences of the second Kunitz-modules of the human and fugu WFIKKN proteins (wfikkn_hu_2; wfikkn_fugu_2) with the second Kunitz-domains of the human and the two fugu WFIKKNRP ... determinant of the hydrophobic S¢2 binding pocket of trypsins is the side-chain of Tyr151 [18] The importance of this residue is underlined by the fact that in the case of the second Kunitz-domain of...
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