TR NG I H C C N TH KHOA NÔNG NGHI P & SINH H C NG D NG TR N HOÀI LÊN HI U QU C A INTERFERON ALPHA VÀ INTERFERON GAMMA GÀ BI U HI N TRÊN TH NG PICHIA PASTORIS TRONG PHÒNG VÀ U TR B NH GUMBORO TRÊN GÀ TU N TU I Lu n v n t t nghi p Ngành: THÚ Y n Th , 2013 TR NG I H C C N TH KHOA NÔNG NGHI P & SINH H C NG D NG Lu n v n t t nghi p Ngành: THÚ Y Tên tài: HI U QU C A INTERFERON ALPHA VÀ INTERFERON GAMMA GÀ BI U HI N TRÊN TH NG PICHIA PASTORIS TRONG PHÒNG VÀ U TR B NH GUMBORO TRÊN GÀ TU N TU I Giáo viên h ng d n: H Th Vi t Thu Sinh viên th c hi n: Tr n Hoài Lên MSSV: 3092673 L p: Thú Y K35 n Th , 2013 TR NG I H C C N TH KHOA NÔNG NGHI P & SINH H C NG D NG MÔN THÚ Y tài: “Hi u qu c a interferon alpha interferon gamma gà bi u hi n h th ng Pichia pastoris phòng u tr b nh Gumboro gà tu n tu i” sinh viên Tr n Hoài Lên th c hi n t i tr i ch n nuôi th c nghi m phòng thí nghi m virus h c, B môn Thú Y, Khoa Nông Nghi p & Sinh H c ng ng, Tr ng i H c C n Th t tháng 12 n m 2012 n tháng n m 2013. n Th , ngày tháng n m 2013 C n Th , ngày tháng . n m 2013 Duy t B môn Thú Y Duy t Giáo viên h PGS.TS n Th , ngày tháng n m 2013 Duy t Khoa Nông Nghi p & SH D i ng d n TH VI T THU I CAM OAN Tôi xin cam oan nh ng k t qu trình bày lu n v n công trình nghiên u c a b n thân tôi. t c s li u, k t qu hoàn toàn trung th c ch a t ng b t k lu n v n tr c ây. c công b Tác gi lu n v n Tr n Hoài Lên ii IC M N Xin chân thành c m n: Xin kính dâng lên ông bà, cha m lòng bi t n sâu s c s quý tr ng nh t, nh ng ng i c g ng t o m i u ki n t t th c hi n c hoài b o c a mình. Cô H Th Vi t Thu ã t n tâm h hoàn thành t t lu n v n t t nghi p này. Cô Nguy n Th Bé M m h c qua. ng d n giúp i c v n h c t p t o u ki n t t cho ng viên nh c nh su t Quý Th y, Cô B môn Thú Y Ch n Nuôi Tr ng i H c C n Th truy n t ki n th c cho su t th i gian h c t i tr ng. Ch Hu nh Ng c Trang ã h t lòng ch d n t o th i gian làm vi c t i phòng thí nghi m. ã t n tình u ki n thu n l i cho Ch Nguy n Th Thanh Giang (Trung tâm Công ngh Sinh h c Thành ph H Chí Minh) ã h t lòng h ng d n trình th c hi n lu n v n này. Anh Tr n Khánh Long ã nhi t tình giúp hi n tài. ng viên su t th i gian th c p th l p Thú Y K35, anh ch em ngành Thú Y ã giúp ng viên trình h c t p th c hi n tài , chia s Xin kính g i n quý Th y, Cô, anh ch , ng i thân b n bè l i chúc s c kh e, thành công xin nh n n i lòng bi t n sâu s c. n Th , ngày tháng n m 2013. Sinh viên th c hi n Tr n Hoài Lên iii tài: CL C Trang ph bìa .i Trang ký t .ii I CAM OAN .ii IC M N iii C L C .iv DANH M C CH VI T T T .vii DANH SÁCH B NG ix DANH SÁCH HÌNH x TÓM L C xi Ch ng Ch ng C TV N S .1 LÝ LU N .3 2.1 Interferon 2.1.1. S l c v interferon . 2.1.2 Phân lo i interferon 2.1.3 Ho t tính sinh h c c a interferon . 2.2 Interferon gà .5 2.2.1. Phân lo i interferon gà . 2.2.2 Interferon-alpha gà 2.2.3 Interferon -gamma gà 2.2.4 S bi u hi n c a interferon-alpha interferon-gamma gà th ng Pichia pastoris 2.2.5 Các nghiên c u s d ng interferon-alpha interpheron-gamma gà tái t h p gia c m 2.3 B nh Gumboro 2.3.1 Khái ni m v b nh Gumboro 2.3.2 c m hình thái, c u trúc c a virus Gumboro 2.3.3 C ch sinh b nh 10 2.3.4 Tri u ch ng b nh tích 11 2.3.4.1 Tri u ch ng .11 2.3.4.2 B nh tích .12 2.3.5 Phòng b nh u tr 13 iv 2.3.5.1 Phòng b nh .13 2.3.5.2 u tr 14 2.4 Các y u t nh h ng n kh n ng nhi m b nh Gumboro gà 15 2.4.1 Gi ng gà . 15 2.4.2 Tu i gà . 15 2.4.3 Gi i tính gà 15 2.5 Tình hình nghiên c u ChIFN n Ch c .15 2.5.1 Trong n c . 15 2.5.2 Ngoài n c . 16 ng N I DUNG VÀ PH NG PHÁP THÍ NGHI M .19 3.1 N i dung thí nghi m 19 3.2 Ph ng ti n thí nghi m 19 3.2.1 Th i gian a m . 19 3.2.2 V t li u thí nghi m 19 3.3 Ph ng pháp thí nghi m 20 3.3.1 Ph ng pháp chu n b huy n d ch b nh ph m 20 3.3.2 Chu n 3.3.3 Xác 3.3.4 huy n d ch virus Gumboro 20 nh c l c c a virus Gumboro gà tu n tu i 21 ánh giá hi u qu phòng u tr b nh Gumboro c a rChIFN- rChIFN- gà tu n tu i 21 3.4 Ph ng pháp xét nghi m .23 3.4.1 Ph ng pháp ki m tra virus Gumboro b ng ph n ng k t t a khu ch tán th ch (Agar gel precipitation, AGP) . 23 3.4.2 Ph ng pháp ki m tra kháng th kháng virus Gumboro b ng ph n ng ELISA (Enzyme Linked Immuno Sorbent Assay) 24 3.5 Các ch tiêu theo dõi 27 3.6 Ph Ch ng pháp x lý s li u 28 ng K T QU VÀ TH O LU N 29 4.1 Chu n xác nh ELD50 phôi gà 29 4.2 K t qu kh o sát kh n ng gây b nh c a virus Gumboro gà tu n tu i 30 4.3 K t qu ánh giá hi u qu phòng u tr b nh Gumboro c a rChIFN- rChIFN- gà tu n tu i .31 v 4.4 K t qu theo dõi tri u ch ng lâm sàng gà thí nghi m .32 4.5 K t qu kh o sát b nh tích qua m khám .32 4.6 K t qu ki m tra virus Gumboro b ng ph n ng k t t a khu ch tán th ch AGP (Agar gel precipitation) .34 4.7 K t qu ki m tra áp ng mi n d ch v i virus Gumboro b ng ph n ng ELISA 34 4.8 K t qu theo dõi tr ng l ng c a gà thí nghi m tr c sau gây nhi m IBDV .38 Ch ng K T LU N VÀ NGH .41 5.1 K t lu n .41 5.2 ngh 41 TÀI LI U THAM KH O .42 vi DANH SÁCH CH Vi t t t VI T T T Nguyên ch Ngh a ti ng vi t AGP Agarose gel precipitation AIV Avian influenza virus bps Base pairs cAMP Cyclic adenosine monophosphate cDNA Complementary deoxyribonucleic acid CEF Chicken embryo fibroblast ChIFN Chicken interferon Interferon gà COS Cell line derived from kidney cells Dòng t bào t t bào th n c a kh of the African green monkey xanh Châu Phi Ctv DNA C t t a khu ch tán th ch Virus cúm gia c m bào x phôi gà ng tác viên Deoxyribonucleic acid i ch ng ELD50 Embryo lethal dose 50% Li u gây ch t 50% phôi ELISA Enzyme linked ìmmuno sorbent assay Ph n ng mi n d ch g n men GBP Guanylate-binding protein Protein g n k t Guanylate HLA Human leukocyte antigen Kháng nguyên b ch c u ng IBDV Infectious bursal disease virus Virus gây b nh Gumboro IBV Infectious bronchitis virus Virus gây viêm ph qu n truy n i nhi m IFN Interferon IRF Interferon regulatory factor ut IU International unit n v qu c t MAF Macrophage activating factor Tác nhân ho t hóa MaIFN Mammalian interferon Interferon MDV Marek’s disease virus Virus gây b nh Marek's MHC Major histocompatibility complex Ph c h p hòa h p t ch c mRNA Messenger ribonucleic acid RNA thông tin NC Negative control Trung bình KT Kháng th NK Natural kill TuIFN Turkey interferon TB i th c bào vii u hòa interferon i th c bào loài h u nh i ch ng âm RSV Rous sarcoma virus SFV Semliki forest virus VP Viral protein IBD Infectious bursal dísease VSV Vesicular stomatitis virus NDV Newcastle disease virus viii T-Test of difference = (vs not =): T-Value = -17.44 P-Value = 0.000 DF = 10 v Tu n sau thí nghi m Two-Sample T-Test and CI Sample N 8 Mean 3827 112 StDev 1590 105 SE Mean 562 37 Difference = mu (1) - mu (2) Estimate for difference: 3715 95% CI for difference: (2383, 5047) T-Test of difference = (vs not =): T-Value = 6.59 P-Value = 0.000 DF = P-Value = 0.128 DF = Two-Sample T-Test and CI Sample N 8 Mean 3827 2853 StDev 1590 164 SE Mean 562 58 Difference = mu (1) - mu (2) Estimate for difference: 974 95% CI for difference: (-362, 2310) T-Test of difference = (vs not =): T-Value = 1.72 Two-Sample T-Test and CI Sample N 8 Mean 112 2853 StDev SE Mean 105 37 164 58 Difference = mu (1) - mu (2) Estimate for difference: -2741.0 95% CI for difference: (-2892.5, -2589.5) T-Test of difference = (vs not =): T-Value = -39.81 P-Value = 0.000 DF = 11 v Tu n sau thí nghi m Two-Sample T-Test and CI Sample N 8 Mean 2240 36.0 StDev 701 17.0 SE Mean 248 6.0 Difference = mu (1) - mu (2) Estimate for difference: 2204 95% CI for difference: (1618, 2790) T-Test of difference = (vs not =): T-Value = 8.89 P-Value = 0.000 DF = P-Value = 0.125 DF = 10 Two-Sample T-Test and CI Sample N 8 Mean 2240 1777 StDev 701 348 SE Mean 248 123 Difference = mu (1) - mu (2) Estimate for difference: 463 95% CI for difference: (-154, 1080) T-Test of difference = (vs not =): T-Value = 1.67 Two-Sample T-Test and CI Sample N 8 Mean 36.0 1777 StDev 17.0 348 SE Mean 6.0 123 Difference = mu (1) - mu (2) Estimate for difference: -1741 95% CI for difference: (-2032, -1450) 59 T-Test of difference = (vs not =): T-Value = -14.13 P-Value = 0.000 DF = v Tu n sau thí nghi m Two-Sample T-Test and CI Sample N 8 Mean 1495 21.00 StDev 563 6.00 SE Mean 199 2.1 Difference = mu (1) - mu (2) Estimate for difference: 1474 95% CI for difference: (1003, 1945) T-Test of difference = (vs not =): T-Value = 7.40 P-Value = 0.000 DF = P-Value = 0.087 DF = Two-Sample T-Test and CI Sample N 8 Mean 1495 1087 StDev 563 144 SE Mean 199 51 Difference = mu (1) - mu (2) Estimate for difference: 408 95% CI for difference: (-78, 894) T-Test of difference = (vs not =): T-Value = 1.99 Two-Sample T-Test and CI Sample N 8 Mean 21.00 1087 StDev 6.00 144 SE Mean 2.1 51 Difference = mu (1) - mu (2) Estimate for difference: -1066.0 95% CI for difference: (-1186.5, -945.5) T-Test of difference = (vs not =): T-Value = -20.92 ng 4.13 K t qu tr ng l ng trung bình tr nghi m phòng b nh Gumboro P-Value = 0.000 DF = c sau thí nghi m thí Ngày Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N Mean StDev SE Mean NT 30 282.5 11.1 2.0 NT 30 282.5 10.9 2.0 Difference = mu (NT 1) - mu (NT 3) Estimate for difference: 0.00 95% CI for difference: (-5.69, 5.69) T-Test of difference = (vs not =): T-Value = 0.00 Both use Pooled StDev = 11.0055 P-Value = 1.000 DF = 58 P-Value = 0.954 DF = 58 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 282.5 11.1 282.3 11.3 SE Mean 2.0 2.1 Difference = mu (NT 1) - mu (NT 4) Estimate for difference: 0.17 95% CI for difference: (-5.62, 5.95) T-Test of difference = (vs not =): T-Value = 0.06 Both use Pooled StDev = 11.1990 60 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 282.5 11.1 282.17 9.53 SE Mean 2.0 1.7 Difference = mu (NT 1) - mu (NT 5) Estimate for difference: 0.33 95% CI for difference: (-5.02, 5.69) T-Test of difference = (vs not =): T-Value = 0.12 Both use Pooled StDev = 10.3571 P-Value = 0.901 DF = 58 P-Value = 0.954 DF = 58 P-Value = 0.900 DF = 58 P-Value = 0.951 DF = 58 P-Value = 0.025 DF = 58 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 282.5 10.9 282.3 11.3 SE Mean 2.0 2.1 Difference = mu (NT 3) - mu (NT 4) Estimate for difference: 0.17 95% CI for difference: (-5.56, 5.89) T-Test of difference = (vs not =): T-Value = 0.06 Both use Pooled StDev = 11.0829 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 282.5 10.9 282.17 9.53 SE Mean 2.0 1.7 Difference = mu (NT 3) - mu (NT 5) Estimate for difference: 0.33 95% CI for difference: (-4.95, 5.62) T-Test of difference = (vs not =): T-Value = 0.13 Both use Pooled StDev = 10.2315 Two-Sample T-Test and CI: NT 3, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 282.3 11.3 282.17 9.53 SE Mean 2.1 1.7 Difference = mu (NT 4) - mu (NT 5) Estimate for difference: 0.17 95% CI for difference: (-5.23, 5.56) T-Test of difference = (vs not =): T-Value = 0.06 Both use Pooled StDev = 10.4393 Ngày Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 331.7 12.7 323.7 14.1 SE Mean 2.3 2.6 Difference = mu (NT 1) - mu (NT 3) Estimate for difference: 8.00 95% CI for difference: (1.06, 14.94) T-Test of difference = (vs not =): T-Value = 2.31 Both use Pooled StDev = 13.4314 61 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 331.7 12.7 322.7 14.7 SE Mean 2.3 2.7 Difference = mu (NT 1) - mu (NT 4) Estimate for difference: 9.00 95% CI for difference: (1.91, 16.09) T-Test of difference = (vs not =): T-Value = 2.54 Both use Pooled StDev = 13.7109 P-Value = 0.014 DF = 58 P-Value = 0.914 DF = 58 P-Value = 0.789 DF = 58 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 331.7 12.7 331.3 11.1 SE Mean 2.3 2.0 Difference = mu (NT 1) - mu (NT 5) Estimate for difference: 0.33 95% CI for difference: (-5.82, 6.48) T-Test of difference = (vs not =): T-Value = 0.11 Both use Pooled StDev = 11.9000 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 323.7 14.1 322.7 14.7 SE Mean 2.6 2.7 Difference = mu (NT 3) - mu (NT 4) Estimate for difference: 1.00 95% CI for difference: (-6.44, 8.44) T-Test of difference = (vs not =): T-Value = 0.27 Both use Pooled StDev = 14.4039 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 323.7 14.1 331.3 11.1 SE Mean 2.6 2.0 Difference = mu (NT 3) - mu (NT 5) Estimate for difference: -7.67 95% CI for difference: (-14.23, -1.11) T-Test of difference = (vs not =): T-Value = -2.34 Both use Pooled StDev = 12.6922 P-Value = 0.023 DF = 58 P-Value = 0.012 DF = 58 Two-Sample T-Test and CI: NT 3, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 322.7 14.7 331.3 11.1 SE Mean 2.7 2.0 Difference = mu (NT 4) - mu (NT 5) Estimate for difference: -8.67 95% CI for difference: (-15.38, -1.95) T-Test of difference = (vs not =): T-Value = -2.58 Both use Pooled StDev = 12.9876 62 Ngày Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 369.5 15.2 358.7 16.6 SE Mean 2.8 3.0 Difference = mu (NT 1) - mu (NT 3) Estimate for difference: 10.83 95% CI for difference: (2.60, 19.07) T-Test of difference = (vs not =): T-Value = 2.63 Both use Pooled StDev = 15.9277 P-Value = 0.011 DF = 58 P-Value = 0.628 DF = 58 P-Value = 0.928 DF = 58 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 369.5 15.2 367.3 19.0 SE Mean 2.8 3.5 Difference = mu (NT 1) - mu (NT 4) Estimate for difference: 2.17 95% CI for difference: (-6.73, 11.07) T-Test of difference = (vs not =): T-Value = 0.49 Both use Pooled StDev = 17.2228 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 369.5 15.2 369.2 13.3 SE Mean 2.8 2.4 Difference = mu (NT 1) - mu (NT 5) Estimate for difference: 0.33 95% CI for difference: (-7.06, 7.73) T-Test of difference = (vs not =): T-Value = 0.09 Both use Pooled StDev = 14.3068 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 358.7 16.6 367.3 19.0 SE Mean 3.0 3.5 Difference = mu (NT 3) - mu (NT 4) Estimate for difference: -8.67 95% CI for difference: (-17.89, 0.56) T-Test of difference = (vs not =): T-Value = -1.88 Both use Pooled StDev = 17.8516 P-Value = 0.065 DF = 58 P-Value = 0.009 DF = 58 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 358.7 16.6 369.2 13.3 SE Mean 3.0 2.4 Difference = mu (NT 3) - mu (NT 5) Estimate for difference: -10.50 95% CI for difference: (-18.28, -2.72) T-Test of difference = (vs not =): T-Value = -2.70 63 Both use Pooled StDev = 15.0578 Two-Sample T-Test and CI: NT 3, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 367.3 19.0 369.2 13.3 SE Mean 3.5 2.4 Difference = mu (NT 4) - mu (NT 5) Estimate for difference: -1.83 95% CI for difference: (-10.32, 6.65) T-Test of difference = (vs not =): T-Value = -0.43 Both use Pooled StDev = 16.4216 P-Value = 0.667 DF = 58 Ngày Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 425.2 18.6 393.8 18.9 SE Mean 3.4 3.4 Difference = mu (NT 1) - mu (NT 3) Estimate for difference: 31.33 95% CI for difference: (21.65, 41.02) T-Test of difference = (vs not =): T-Value = 6.48 Both use Pooled StDev = 18.7351 P-Value = 0.000 DF = 58 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 425.2 18.6 427.0 23.4 SE Mean 3.4 4.3 Difference = mu (NT 1) - mu (NT 4) Estimate for difference: -1.83 95% CI for difference: (-12.77, 9.10) T-Test of difference = (vs not =): T-Value = -0.34 Both use Pooled StDev = 21.1539 P-Value = 0.738 DF = 58 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 425.2 18.6 424.8 16.6 SE Mean 3.4 3.0 Difference = mu (NT 1) - mu (NT 5) Estimate for difference: 0.33 95% CI for difference: (-8.78, 9.45) T-Test of difference = (vs not =): T-Value = 0.07 Both use Pooled StDev = 17.6402 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 393.8 18.9 427.0 23.4 SE Mean 3.4 4.3 Difference = mu (NT 3) - mu (NT 4) Estimate for difference: -33.17 95% CI for difference: (-44.16, -22.17) 64 P-Value = 0.942 DF = 58 T-Test of difference = (vs not =): T-Value = -6.04 Both use Pooled StDev = 21.2798 P-Value = 0.000 DF = 58 P-Value = 0.000 DF = 58 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 393.8 18.9 424.8 16.6 SE Mean 3.4 3.0 Difference = mu (NT 3) - mu (NT 5) Estimate for difference: -31.00 95% CI for difference: (-40.20, -21.80) T-Test of difference = (vs not =): T-Value = -6.75 Both use Pooled StDev = 17.7911 Two-Sample T-Test and CI: NT 3, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 427.0 23.4 424.8 16.6 SE Mean 4.3 3.0 Difference = mu (NT 4) - mu (NT 5) Estimate for difference: 2.17 95% CI for difference: (-8.34, 12.67) T-Test of difference = (vs not =): T-Value = 0.41 Both use Pooled StDev = 20.3225 P-Value = 0.681 DF = 58 P-Value = 0.000 DF = 58 Ngày Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 450.2 22.5 414.8 22.4 SE Mean 4.1 4.1 Difference = mu (NT 1) - mu (NT 3) Estimate for difference: 35.33 95% CI for difference: (23.75, 46.92) T-Test of difference = (vs not =): T-Value = 6.10 Both use Pooled StDev = 22.4178 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 450.2 22.5 486.7 27.9 SE Mean 4.1 5.1 Difference = mu (NT 1) - mu (NT 4) Estimate for difference: -36.50 95% CI for difference: (-49.59, -23.41) T-Test of difference = (vs not =): T-Value = -5.58 Both use Pooled StDev = 25.3223 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 450.2 22.5 445.2 20.2 SE Mean 4.1 3.7 Difference = mu (NT 1) - mu (NT 5) Estimate for difference: 5.00 65 P-Value = 0.000 DF = 58 95% CI for difference: (-6.05, 16.05) T-Test of difference = (vs not =): T-Value = 0.91 Both use Pooled StDev = 21.3745 P-Value = 0.369 DF = 58 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 414.8 22.4 486.7 27.9 SE Mean 4.1 5.1 Difference = mu (NT 3) - mu (NT 4) Estimate for difference: -71.83 95% CI for difference: (-84.90, -58.76) T-Test of difference = (vs not =): T-Value = -11.00 Both use Pooled StDev = 25.2883 P-Value = 0.000 DF = 58 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 414.8 22.4 445.2 20.2 SE Mean 4.1 3.7 Difference = mu (NT 3) - mu (NT 5) Estimate for difference: -30.33 95% CI for difference: (-41.36, -19.31) T-Test of difference = (vs not =): T-Value = -5.51 Both use Pooled StDev = 21.3341 P-Value = 0.000 DF = 58 Two-Sample T-Test and CI: NT 3, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 486.7 27.9 445.2 20.2 SE Mean 5.1 3.7 Difference = mu (NT 4) - mu (NT 5) Estimate for difference: 41.50 95% CI for difference: (28.91, 54.09) T-Test of difference = (vs not =): T-Value = 6.60 Both use Pooled StDev = 24.3682 P-Value = 0.000 DF = 58 P-Value = 0.000 DF = 46 Ngày 10 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 28 20 Mean StDev 490.4 26.2 453.3 24.6 SE Mean 5.0 5.5 Difference = mu (NT 1) - mu (NT 3) Estimate for difference: 37.11 95% CI for difference: (22.04, 52.17) T-Test of difference = (vs not =): T-Value = 4.96 Both use Pooled StDev = 25.5633 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 28 30 Mean StDev 490.4 26.2 546.3 32.4 SE Mean 5.0 5.9 Difference = mu (NT 1) - mu (NT 4) 66 Estimate for difference: -55.98 95% CI for difference: (-71.54, -40.41) T-Test of difference = (vs not =): T-Value = -7.21 Both use Pooled StDev = 29.5632 P-Value = 0.000 DF = 56 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 28 28 Mean StDev 490.4 26.2 480.5 23.9 SE Mean 5.0 4.5 Difference = mu (NT 1) - mu (NT 5) Estimate for difference: 9.82 95% CI for difference: (-3.62, 23.26) T-Test of difference = (vs not =): T-Value = 1.47 Both use Pooled StDev = 25.0789 P-Value = 0.149 DF = 54 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 20 30 Mean StDev 453.3 24.6 546.3 32.4 SE Mean 5.5 5.9 Difference = mu (NT 3) - mu (NT 4) Estimate for difference: -93.08 95% CI for difference: (-110.23, -75.93) T-Test of difference = (vs not =): T-Value = -10.91 Both use Pooled StDev = 29.5488 P-Value = 0.000 DF = 48 Two-Sample T-Test and CI: NT 2, NT4 Two-sample T for NT vs NT N NT NT 20 28 Mean StDev 453.3 24.6 480.5 23.9 SE Mean 5.5 4.5 Difference = mu (NT 3) - mu (NT 5) Estimate for difference: -27.29 95% CI for difference: (-41.54, -13.03) T-Test of difference = (vs not =): T-Value = -3.85 Both use Pooled StDev = 24.1961 P-Value = 0.000 DF = 46 Two-Sample T-Test and CI: NT 3, NT Two-sample T for NT vs NT N NT NT 30 28 Mean StDev 546.3 32.4 480.5 23.9 SE Mean 5.9 4.5 Difference = mu (NT 4) - mu (NT 5) Estimate for difference: 65.80 95% CI for difference: (50.74, 80.85) T-Test of difference = (vs not =): T-Value = 8.75 Both use Pooled StDev = 28.6025 Ngày 12 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 28 20 Mean StDev 545.4 29.5 508.8 27.7 SE Mean 5.6 6.2 67 P-Value = 0.000 DF = 56 Difference = mu (NT 1) - mu (NT 3) Estimate for difference: 36.61 95% CI for difference: (19.67, 53.54) T-Test of difference = (vs not =): T-Value = 4.35 Both use Pooled StDev = 28.7380 P-Value = 0.000 DF = 46 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 28 30 Mean StDev 545.4 29.5 606.0 36.9 SE Mean 5.6 6.7 Difference = mu (NT 1) - mu (NT 4) Estimate for difference: -60.64 95% CI for difference: (-78.28, -43.01) T-Test of difference = (vs not =): T-Value = -6.89 Both use Pooled StDev = 33.5054 P-Value = 0.000 DF = 56 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 28 28 Mean StDev 545.4 29.5 540.2 26.9 SE Mean 5.6 5.1 Difference = mu (NT 1) - mu (NT 5) Estimate for difference: 5.18 95% CI for difference: (-9.95, 20.30) T-Test of difference = (vs not =): T-Value = 0.69 Both use Pooled StDev = 28.2255 P-Value = 0.495 DF = 54 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 20 30 Mean StDev 508.8 27.7 606.0 36.9 SE Mean 6.2 6.7 Difference = mu (NT 3) - mu (NT 4) Estimate for difference: -97.25 95% CI for difference: (-116.71, -77.79) T-Test of difference = (vs not =): T-Value = -10.05 Both use Pooled StDev = 33.5298 P-Value = 0.000 DF = 48 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 20 28 Mean StDev 508.8 27.7 540.2 26.9 SE Mean 6.2 5.1 Difference = mu (NT 3) - mu (NT 5) Estimate for difference: -31.43 95% CI for difference: (-47.48, -15.38) T-Test of difference = (vs not =): T-Value = -3.94 Both use Pooled StDev = 27.2340 Two-Sample T-Test and CI: NT 3, NT Two-sample T for NT vs NT N NT NT 30 28 Mean StDev 606.0 36.9 540.2 26.9 SE Mean 6.7 5.1 68 P-Value = 0.000 DF = 46 Difference = mu (NT 4) - mu (NT 5) Estimate for difference: 65.82 95% CI for difference: (48.74, 82.91) T-Test of difference = (vs not =): T-Value = 7.72 Both use Pooled StDev = 32.4571 P-Value = 0.000 DF = 56 P-Value = 0.001 DF = 46 Ngày 14 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 28 20 Mean StDev 605.2 33.9 569.3 31.3 SE Mean 6.4 7.0 Difference = mu (NT 1) - mu (NT 3) Estimate for difference: 35.93 95% CI for difference: (16.58, 55.28) T-Test of difference = (vs not =): T-Value = 3.74 Both use Pooled StDev = 32.8329 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 28 30 Mean StDev 605.2 33.9 666.0 41.0 SE Mean 6.4 7.5 Difference = mu (NT 1) - mu (NT 4) Estimate for difference: -60.82 95% CI for difference: (-80.70, -40.95) T-Test of difference = (vs not =): T-Value = -6.13 Both use Pooled StDev = 37.7596 P-Value = 0.000 DF = 56 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 28 28 Mean StDev 605.2 33.9 601.8 30.0 SE Mean 6.4 5.7 Difference = mu (NT 1) - mu (NT 5) Estimate for difference: 3.39 95% CI for difference: (-13.75, 20.54) T-Test of difference = (vs not =): T-Value = 0.40 Both use Pooled StDev = 31.9968 P-Value = 0.693 DF = 54 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 20 30 Mean StDev 569.3 31.3 666.0 41.0 SE Mean 7.0 7.5 Difference = mu (NT 3) - mu (NT 4) Estimate for difference: -96.8 95% CI for difference: (-118.5, -75.0) T-Test of difference = (vs not =): T-Value = -8.95 Both use Pooled StDev = 37.4677 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 20 28 Mean StDev 569.3 31.3 601.8 30.0 SE Mean 7.0 5.7 69 P-Value = 0.000 DF = 48 Difference = mu (NT 3) - mu (NT 5) Estimate for difference: -32.54 95% CI for difference: (-50.52, -14.55) T-Test of difference = (vs not =): T-Value = -3.64 Both use Pooled StDev = 30.5117 P-Value = 0.001 DF = 46 Two-Sample T-Test and CI: NT 3, NT Two-sample T for NT vs NT N NT NT 30 28 Mean StDev 666.0 41.0 601.8 30.0 SE Mean 7.5 5.7 Difference = mu (NT 4) - mu (NT 5) Estimate for difference: 64.21 95% CI for difference: (45.20, 83.23) T-Test of difference = (vs not =): T-Value = 6.76 Both use Pooled StDev = 36.1250 ng 4.14 K t qu tr ng l ng trung bình tr nghi m u tr b nh Gumboro P-Value = 0.000 DF = 56 c sau thí nghi m thí Ngày Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 323.2 14.8 322.7 14.7 SE Mean 2.7 2.7 Difference = mu (NT 2) - mu (NT 4) Estimate for difference: 0.50 95% CI for difference: (-7.12, 8.12) T-Test of difference = (vs not =): T-Value = 0.13 Both use Pooled StDev = 14.7454 P-Value = 0.896 DF = 58 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 323.2 14.8 331.3 11.1 SE Mean 2.7 2.0 Difference = mu (NT 2) - mu (NT 5) Estimate for difference: -8.17 95% CI for difference: (-14.93, -1.41) T-Test of difference = (vs not =): T-Value = -2.42 Both use Pooled StDev = 13.0786 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 322.7 14.7 331.3 11.1 SE Mean 2.7 2.0 Difference = mu (NT 4) - mu (NT 5) Estimate for difference: -8.67 70 P-Value = 0.019 DF = 58 95% CI for difference: (-15.38, -1.95) T-Test of difference = (vs not =): T-Value = -2.58 P-Value = 0.012 DF = 58 P-Value = 0.073 DF = 58 P-Value = 0.013 DF = 58 P-Value = 0.667 DF = 58 P-Value = 0.082 DF = 58 Both use Pooled StDev = 12.9876 Ngày Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 358.5 18.4 367.3 19.0 SE Mean 3.4 3.5 Difference = mu (NT 2) - mu (NT 4) Estimate for difference: -8.83 95% CI for difference: (-18.50, 0.83) T-Test of difference = (vs not =): T-Value = -1.83 Both use Pooled StDev = 18.7056 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 358.5 18.4 369.2 13.3 SE Mean 3.4 2.4 Difference = mu (NT 2) - mu (NT 5) Estimate for difference: -10.67 95% CI for difference: (-18.97, -2.37) T-Test of difference = (vs not =): T-Value = -2.57 Both use Pooled StDev = 16.0611 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 367.3 19.0 369.2 13.3 SE Mean 3.5 2.4 Difference = mu (NT 4) - mu (NT 5) Estimate for difference: -1.83 95% CI for difference: (-10.32, 6.65) T-Test of difference = (vs not =): T-Value = -0.43 Both use Pooled StDev = 16.4216 Ngày Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 416.7 21.7 427.0 23.4 SE Mean 4.0 4.3 Difference = mu (NT 2) - mu (NT 4) Estimate for difference: -10.33 95% CI for difference: (-22.02, 1.35) T-Test of difference = (vs not =): T-Value = -1.77 Both use Pooled StDev = 22.6086 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N Mean StDev SE Mean 71 NT NT 30 30 416.7 424.8 21.7 16.6 4.0 3.0 Difference = mu (NT 2) - mu (NT 5) Estimate for difference: -8.17 95% CI for difference: (-18.17, 1.84) T-Test of difference = (vs not =): T-Value = -1.63 Both use Pooled StDev = 19.3608 P-Value = 0.108 DF = 58 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 427.0 23.4 424.8 16.6 SE Mean 4.3 3.0 Difference = mu (NT 4) - mu (NT 5) Estimate for difference: 2.17 95% CI for difference: (-8.34, 12.67) T-Test of difference = (vs not =): T-Value = 0.41 Both use Pooled StDev = 20.3225 P-Value = 0.681 DF = 58 Ngày Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 27 30 Mean StDev 452.6 25.2 486.7 27.9 SE Mean 4.9 5.1 Difference = mu (NT 2) - mu (NT 4) Estimate for difference: -34.07 95% CI for difference: (-48.25, -19.90) T-Test of difference = (vs not =): T-Value = -4.82 Both use Pooled StDev = 26.6578 P-Value = 0.000 DF = 55 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 27 30 Mean StDev 452.6 25.2 445.2 20.2 SE Mean 4.9 3.7 Difference = mu (NT 2) - mu (NT 5) Estimate for difference: 7.43 95% CI for difference: (-4.65, 19.50) T-Test of difference = (vs not =): T-Value = 1.23 Both use Pooled StDev = 22.7207 P-Value = 0.223 DF = 55 P-Value = 0.000 DF = 58 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 30 30 Mean StDev 486.7 27.9 445.2 20.2 SE Mean 5.1 3.7 Difference = mu (NT 4) - mu (NT 5) Estimate for difference: 41.50 95% CI for difference: (28.91, 54.09) T-Test of difference = (vs not =): T-Value = 6.60 Both use Pooled StDev = 24.3682 72 Ngày Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 27 30 Mean StDev 497.4 28.5 546.3 32.4 SE Mean 5.5 5.9 Difference = mu (NT 2) - mu (NT 4) Estimate for difference: -48.93 95% CI for difference: (-65.19, -32.66) T-Test of difference = (vs not =): T-Value = -6.03 Both use Pooled StDev = 30.5897 P-Value = 0.000 DF = 55 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 27 28 Mean StDev 497.4 28.5 480.5 23.9 SE Mean 5.5 4.5 Difference = mu (NT 2) - mu (NT 5) Estimate for difference: 16.87 95% CI for difference: (2.68, 31.07) T-Test of difference = (vs not =): T-Value = 2.38 Both use Pooled StDev = 26.2375 P-Value = 0.021 DF = 53 P-Value = 0.000 DF = 56 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 30 28 Mean StDev 546.3 32.4 480.5 23.9 SE Mean 5.9 4.5 Difference = mu (NT 4) - mu (NT 5) Estimate for difference: 65.80 95% CI for difference: (50.74, 80.85) T-Test of difference = (vs not =): T-Value = 8.75 Both use Pooled StDev = 28.6025 Ngày 10 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 27 30 Mean StDev 553.5 30.1 606.0 36.9 SE Mean 5.8 6.7 Difference = mu (NT 2) - mu (NT 4) Estimate for difference: -52.48 95% CI for difference: (-70.46, -34.50) T-Test of difference = (vs not =): T-Value = -5.85 Both use Pooled StDev = 33.8206 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT 73 P-Value = 0.000 DF = 55 N NT NT 27 28 Mean StDev 553.5 30.1 540.2 26.9 SE Mean 5.8 5.1 Difference = mu (NT 2) - mu (NT 5) Estimate for difference: 13.34 95% CI for difference: (-2.08, 28.76) T-Test of difference = (vs not =): T-Value = 1.74 Both use Pooled StDev = 28.5052 P-Value = 0.089 DF = 53 P-Value = 0.000 DF = 56 Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 30 28 Mean StDev 606.0 36.9 540.2 26.9 SE Mean 6.7 5.1 Difference = mu (NT 4) - mu (NT 5) Estimate for difference: 65.82 95% CI for difference: (48.74, 82.91) T-Test of difference = (vs not =): T-Value = 7.72 Both use Pooled StDev = 32.4571 Ngày 12 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 26 30 Mean StDev 615.6 31.5 666.0 41.0 SE Mean 6.2 7.5 Difference = mu (NT 2) - mu (NT 4) Estimate for difference: -50.42 95% CI for difference: (-70.27, -30.58) T-Test of difference = (vs not =): T-Value = -5.09 Both use Pooled StDev = 36.9400 P-Value = 0.000 DF = 54 Two-Sample T-Test and CI: NT 1, NT Two-sample T for NT vs NT N NT NT 26 28 Mean StDev 615.6 31.5 601.8 30.0 SE Mean 6.2 5.7 Difference = mu (NT 2) - mu (NT 5) Estimate for difference: 13.79 95% CI for difference: (-3.01, 30.59) T-Test of difference = (vs not =): T-Value = 1.65 Both use Pooled StDev = 30.7368 P-Value = 0.105 DF = 52 P-Value = 0.000 DF = 56oth Two-Sample T-Test and CI: NT 2, NT Two-sample T for NT vs NT N NT NT 30 28 Mean StDev 666.0 41.0 601.8 30.0 SE Mean 7.5 5.7 Difference = mu (NT 4) - mu (NT 5) Estimate for difference: 64.21 95% CI for difference: (45.20, 83.23) T-Test of difference = (vs not =): T-Value = 6.76 use Pooled StDev = 36.1250 74 [...]... và ChIFN- tái t h p bi u hi n trên h th ng P pastoris Sinh ph m này n c xác nh hi u qu phòng và u tr in vivo các b nh do virus gây ra trên gà trong ó có b nh Gumboro Trên c s ó, chúng tôi ti n hành th c hi n tài: "Hi u qu c a interferon alpha và interferon gamma gà bi u hi n trên h th ng Pichia pastoris trong phòng và u tr b nh Gumboro trên gà 3 tu n tu i" 1 c tiêu tài: −Kh o sát kh n ng phòng và trên. .. l phôi gà ch t 4.2 T l gà m c b nh và gà ch t 4 .3 l gà m c b nh và t l gà ch t các nghi m th c trong vi c phòng b nh Gumboro 31 4.4 l gà m c b nh và t l gà ch t các nghi m th c trong vi c u tr b nh Gumboro 31 4.5 T n su t xu t hi n tri u ch ng trên gà thí nghi m m c b nh 32 4.6 T n su t xu t hi n b nh tích trên gà ch t trong thí nghi m 33 4.7 t qu ki m tra virus Gumboro. .. Pichia pastoris (P pastoris) trong phòng và u tr b nh Gumboro trên gà 3 tu n tu i qua ba thí nghi m: ki m tra c l c c a virus Gumboro, ánh giá kh n ng phòng b nh Gumboro c a rChIFN- và rChIFN- trên gà 3 tu n tu i, và ánh giá kh n ng u tr b nh Gumboro c a rChIFN- và rChIFN- trên gà 3 tu n tu i Thí nghi m ki m tra c l c c a virus Gumboro g m 2 nghi m th c, nghi m th c virus (gà c gây nhi m v i virus Gumboro) ... gamma gà bi u hi n trên h th ng Pichia pastoris trong phòng và u tr b nh Gumboro trên gà 3 tu n tu i" c th c hi n t i tr i ch n nuôi th c nghi m và phòng thí nghi m virus h c, B môn Thú Y, Khoa Nông nghi p và Sinh h c ng d ng, tr ng i h c C n Th t tháng 08/20 13 n 12/20 13, trong tài này, chúng tôi ánh giá hi u qu c a interferon alpha gà (ChIFN- ) và interferon gamma gà (ChIFN- ) bi u hi n trên h th ng Pichia. .. ch s ELD50 Trang 3. 1 B trí thí nghi m xác 3. 2 trí thí nghi m kh o sát c l c c a virus Gumboro trên gà 3 tu n tu i gà 22 3. 3 trí thí nghi m ánh giá hi u qu phòng b nh Gumboro c a rChIFN- và rChIFN- trên gà 3 tu n tu i 22 3. 4 trí thí nghi m ánh giá hi u qu u tr b nh Gumboro c a rChIFN- và rChIFN- trên gà 3 tu n tu i 23 3.5 B trí thí nghi m m u huy t thanh trong xét nghi m ELISA... nh Gumboro 10 2 .3 C ch sinh b nh c a virus Gumboro 11 3. 1 M u th ch dùng 4.1 Gà rúc 4.2 Gà 4 .3 Xu t huy t c 4.4 Xu t huy t c ng c 33 4.5 Xu t huy t tuy n c 33 4.6 Th n s ng 33 4.7 K t qu xét nghi m ELISA 34 4.8 Bi u hi u giá kháng th c a gà tr c và sau trong vi c phòng nh Gumboro 37 4.9 Bi u hi u giá kháng th c a gà tr c và sau trong. .. tr nh Gumboro 37 4.10 Bi u tr ng l ng trung bình c a gà các nghi m th c trong trong vi c phòng b nh Gumboro …………………………… ………… 39 4.11 Bi u vi c 24 làm ph n ng AGP u vào cánh 32 r , n m ph ph c 32 ùi 33 tr ng l ng trung bình c a gà các nghi m th c trong trong u tr b nh Gumboro ……………………………………… 40 x TÓM L C tài: "Hi u qu c a interferon alpha và interferon. .. bào co Ch I DUNG VÀ PH ng 3 NG PHÁP THÍ NGHI M 3. 1 N i dung thí nghi m Chu n và xác nh c l c c a virus Gumboro trên phôi gà b ng ph xác nh li u gây ch t 50% phôi (ELD50 - Embryo lethal dose 50%) Kh o sát c l c c a virus Gumboro trên gà 3 tu n tu i Xác nh hi u qu phòng và rChIFN- trên gà 3 tu n tu i 3. 2 Ph ng pháp u tr b nh Gumboro c a d ch rChIFN- và ng ti n thí nghi m 3. 2.1 Th i gian và a m th c hi... u/con ng c p ngày c p (1 ngày 1 l n) 30 100µg + 1µg Nh m t, m i 3 30 10µg + 1µg Nh m t, m i 3 30 30 0,2ml PBS 0,2ml virus U ng U ng 1 1 rChIFN: interferon gà tái t h p Thí nghi m 2: c tiêu: xác nh hi u qu c a rChIFN- và rChIFN- trong vi c Gumboro trên gà 3 tu n tu i u tr b nh B trí thí nghi m: gà 3 tu n tu i (không có kháng th Gumboro) c b trí ng u nhiên thành 3 nghi m th c, m i nghi m th c 30 con Nghi... thanh gà thí ngh m ki m tra kháng th th ng kháng virus Gumboro b ng ph n ng ELISA, nh ng gà c dùng thí nghi m ph i có k t qu âm tính 21 B ng 3. 2 B trí thí nghi m kh o sát Nghi m th c C(PBS) Virus S gà TN 30 30 c l c c a virus Gumboro trên gà 3 tu n tu i Li u/con 0,2ml 0,2ml ng c p ng ng C: i ch ng TN: Thí nhi m 3. 3.4 ánh giá hi u qu phòng và rChIFN- trên gà 3 tu n tu i u tr b nh Gumboro c a rChIFN- và . 8 2.3 Bnh Gumboro 9 2.3.1 Khái nim v bnh Gumboro 9 2.3.2 c m hình thái, cu trúc ca virus Gumboro 9 2.3.3 C ch sinh bnh 10 2.3.4 Triu chng và bnh tích 11 2.3.4.1 Triu chng 11 2.3.4.2. ng min dch vi virus Gumboro bng phn ng ELISA 34 4 .8 Kt qu theo dõi trng lng ca gà thí nghim trc và sau khi gây nhim IBDV 38 Chng 5 KT LUN VÀ  NGH 41 5.1 Kt lun 41 5.2 . gm IFN- và INF-. IFN- có ít nht 15 type ph có khi lng phân t khong 18 kDa. Các gene mã hóa ca chúng có 85 % tính tng ng. Ngun t bào chính sn xut IFN- là bch cu n nhân. IFN-