69 Kết quả đọc trình tự của mồi M31 trên mẫu Mỹ Hiệp 70 Sự tƣơng đồng giữa hai mồi P14 và M31 của mẫu Mỹ Hiệp CLUSTAL X (1.83) multiple sequence alignment 25 ABI3100_Ic-P14_008_2005-08-2 1 CTTGCTTGATCGCTATGTCGATAG AAAAGATAACAAAAAGAAGAAAAAGA 50 ABI3100_Ic-M31_010_2005-08-2 ABI3100_Ic-P14_008_2005-08-2 51 TAAACAAAAAGACAAGAATTCATGAAGGTACTAGTGACGGAAACAATGTG 100 ABI3100_Ic-M31_010_2005-08-2 ABI3100_Ic-P14_008_2005-08-2 101 TCAACTAGTACCAAAACTGGAGAGAGAGATAGAGATGTCAATGCCGGAAC 150 ABI3100_Ic-M31_010_2005-08-2 ABI3100_Ic-P14_008_2005-08-2 151 TAGTGGAACTTTCACTGTTCCGAGAATAAAATCATTTACTGACAAGATCA 200 ABI3100_Ic-M31_010_2005-08-2 ABI3100_Ic-P14_008_2005-08-2 201 TTTTACCGAAAATTAAAGGAAAAACTGTCCTTAATTTAAATCATCTTCTT 250 ABI3100_Ic-M31_010_2005-08-2 ABI3100_Ic-P14_008_2005-08-2 251 CAGTATAATCCGCAACAAATTGATATCTCAAACACTCGTGCCACTCAAAC 300 ABI3100_Ic-M31_010_2005-08-2 ABI3100_Ic-P14_008_2005-08-2 301 TCAATTCGAAAAGTGGTATGGGGGAGTGAGGAATGATTACGGTCTCAACG 350 ABI3100_Ic-M31_010_2005-08-2 ABI3100_Ic-P14_008_2005-08-2 351 ATAACGAAATGCAAGTGATGTTAAACGGTTTGATGGTTTGGTGTATTGAA 400 ABI3100_Ic-M31_010_2005-08-2 ABI3100_Ic-P14_008_2005-08-2 401 AATGGTACATCTCCAGACTTATCTGGTGTTTGGGTAATGATGGATGGGGA 450 ABI3100_Ic-M31_010_2005-08-2 ABI3100_Ic-P14_008_2005-08-2 451 AACACAAGTTGATTATCCCATTAAGCCTTTAATTGAACATGCAACTCCTT 500 ABI3100_Ic-M31_010_2005-08-2 ABI3100_Ic-P14_008_2005-08-2 501 CGTTTAGGCAAATCATGGCTCACTTCAGTAACGCGGCAGAGGCATACATC 550 ABI3100_Ic-M31_010_2005-08-2 ABI3100_Ic-P14_008_2005-08-2 551 GCGAAGAGGAATGCAACTGAAAGGTATATGCCGCGGTATGGAATCAAGAG 600 ABI3100_Ic-M31_010_2005-08-2 ABI3100_Ic-P14_008_2005-08-2 601 GAATTTGACTGACATTAGCCTCGCTAGATACGCTTTCG ATA 641 ABI3100_Ic-M31_010_2005-08-2 NNNNN 639 71 Phụ lục 9 Kết quả giải trình tự với mồi P14 của mẫu Định Quán 72 Kết quả giải trình từ với mồi M30 của Định Quán 73 Sự tƣơng đồng của hai mồi P7 và M30 của mẫu Định Quán CLUSTAL X (1.83) multiple sequence alignment ABI3100_DQ17-P7_015_2005-08- ABI3100_DQ17-M30_002_2005-08 1 CCTCCCCGCGGCTCTCTCCCGCGAAAAATCTCCCGTTTTAATGGCCCTTT 50 ABI3100_DQ17-P7_015_2005-08- ABI3100_DQ17-M30_002_2005-08 51 TGGGGGAGGGTGTTAATAATATGTTTTTTTTTTTGTGGGGGGCCCCCTCC 100 ABI3100_DQ17-P7_015_2005-08- ABI3100_DQ17-M30_002_2005-08 101 CCCCCCACACACCACTATTTTTTTTTTTTAACCCACAAAAAAAAAACAAC 150 ABI3100_DQ17-P7_015_2005-08- ABI3100_DQ17-M30_002_2005-08 151 CTAAAACCCCTCTTTTTGTGGTGGAGAGAATAGACCCCCCCCCCCCTAAA 200 ABI3100_DQ17-P7_015_2005-08- ABI3100_DQ17-M30_002_2005-08 201 AAATATAAAAAAAGTGATGCGCAGTTGGGGTTACCAATTGGGCTTTGAAG 250 ABI3100_DQ17-P7_015_2005-08- ABI3100_DQ17-M30_002_2005-08 251 AAAAATTTTTTAATATTAACCTTTTCCCCTTATTTTTTTAATATCATCTT 300 341-m30 ABI3100_DQ17-P7_015_2005-08- ABI3100_DQ17-M30_002_2005-08 301 GTCAGTAAATGATTTTATTCTAATCTCGGGACCAGTGGAA CTTTCACGGT 350 1 ABI3100_DQ17-P7_015_2005-08- CGAGAATAAAATCATTTACTGACAAGATGATATTACCAAAAATTAAGG 47 ABI3100_DQ17-M30_002_2005-08 351 TCCGAGAATAAAATCATTTACTGACAAGATGATATTACCAAAAATTAAGG 400 * ** *************** *********************** ABI3100_DQ17-P7_015_2005-08- 48 GAAAAACTGTCCTTAATTTAAATCATCTTCTTCAGTATAATCCGCAACAA 97 ABI3100_DQ17-M30_002_2005-08 401 GAAAAACTGTCCTTAATTTAAATCATCTTCTTCAGTATAATCCGCAACAA 450 ************************************************** ABI3100_DQ17-P7_015_2005-08- 98 ATTGATATCTCAAACACTCGTGCCACTCAAACTCAATTTGAAAAGTGGTA 147 ABI3100_DQ17-M30_002_2005-08 451 ATTGATATCTCAAACACTCGTGCCACTCAAACTCAATTTGAAAAGTGGTA 500 ************************************************** ABI3100_DQ17-P7_015_2005-08- 148 TGAGGGAGTGCGGAATGATTACGGTCTTAACGATGACGAAATGCAAGTGA 197 ABI3100_DQ17-M30_002_2005-08 501 TGAGGGAGTGCGGAATGATTACGGTCTTAACGATGACGAAATGCAAGTGA 550 ************************************************** ABI3100_DQ17-P7_015_2005-08- 198 TGTTAAATGGTTTGATGGTTTGGTGTATCGAAAATGGTACATCTCCAGAC 247 ABI3100_DQ17-M30_002_2005-08 551 TGTTAAATGGTTTGATGGTTTGGTGTATCGAAAATGGTACATCTCCAGAC 600 ************************************************** ABI3100_DQ17-P7_015_2005-08- 248 TTGTCTGGTGTTTGGGTAATGATGGATGGGGAAATACAAGTAGATTATCC 297 ABI3100_DQ17-M30_002_2005-08 601 TTGTCTGGTGTTTGGGTAATGATGGATGGGGAAATACAAGTAGATTATCC 649 *********************** * * ********************** ABI3100_DQ17-P7_015_2005-08- 298 CATCA-AGCCTTTAATTGAACATGCAACTCCCTCGTTTAGGCAAATCATG 346 ABI3100_DQ17-M30_002_2005-08 650 CATCA-AGCCTTTAATTGAACATGCAACTCCCTCGTTTAGGCAAATCATG 699 **** ************* ************************ ***** ABI3100_DQ17-P7_015_2005-08- 347 GCTCACTTCAGTAACGCGGCAGAGGCATACATCGCAAAGAGAATG CCACT 396 ABI3100_DQ17-M30_002_2005-08 701 GCTCACTTCAGTAACGCGGCAGAGGCATACAT ****** ******************* * *732 391-P7 ABI3100_DQ17-P7_015_2005-08- 397 GAGAGGAGTGAAAGTTCCCCTGGTCCCCAAAACGGAGGGGTAGGAAAAGG 446 ABI3100_DQ17-M30_002_2005-08 ABI3100_DQ17-P7_015_2005-08- 447 A ABI3100_DQ17-M30_002_2005-08 - 74 Phụ lục 10 Kết quả giải trình tự với mồi P7 của mẫu Mỹ Hiệp 75 Kết quả giải trình tự với mồi M30 của mẫu Mỹ Hiệp 76 Sự tƣơng đồng của hai mồi P7 và M30 của mẫu Mỹ Hiệp CLUSTAL X (1.83) multiple sequence alignment ABI3100_I-P7_004_2005-08-24 ABI3100_I-M30_006_2005-08-24 1 TGATTTGTAATTACACCACGTTTTCGCCTTCAAACTTTTCTTGGATATGC 50 ABI3100_I-P7_004_2005-08-24 ABI3100_I-M30_006_2005-08-24 51 TCAACAAACTTCTCTGTCCGTGAACAATGGAGCAGTCAAGCAATTGGCGG 100 ABI3100_I-P7_004_2005-08-24 ABI3100_I-M30_006_2005-08-24 101 ATAAGAGGTTTAGGGGTGTGAGTGGGTTTTTCCGGTCAACTCTCTCGGGG 150 1 ABI3100_I-P7_004_2005-08-24 CGATCGA 7 ABI3100_I-M30_006_2005-08-24 151 AGATTCATGTGCTAAATTCGGACCCAGTGGAACTTTCACGGTTCGATCGA 200 * * ABI3100_I-P7_004_2005-08-24 8 GATAATCATTTACTGACA-GATGATTTTACCGAAAATTAAAGGAAAAACT 56 ABI3100_I-M30_006_2005-08-24 201 GATAATCATTTACTGACA-GATGATTTTACCGAAAATTAAAGGAAAAACT 250 * **** ********** ************* ***************** ABI3100_I-P7_004_2005-08-24 57 GTCCTTAATTTTAAATCATCTTCTTCAGTATAATCCGCAACAAATTGATA 106 ABI3100_I-M30_006_2005-08-24 251 GTCCTTAATTTTAAATCATCTTCTTCAGTATAATCCGCAACAAATTGGTA 299 **** ****** *********** ********** ********* ** ABI3100_I-P7_004_2005-08-24 107 TCTCAAACACTCGTGCCACTCAAACTCAATTCGAAAAGTG-GTATGAGGG 155 ABI3100_I-M30_006_2005-08-24 300 TCTCAAACACTCGTGCCTCTCAAACTCAATTCGAAAAGTG-GTATGAGGG 349 ******* ** ***** ******* *** **** * ** ABI3100_I-P7_004_2005-08-24 156 AGTGAGGAATGATTACGGTCTCAACGATAACGAAATGCAAGTG-ATGTTA 204 ABI3100_I-M30_006_2005-08-24 350 AGTGAGGAATGATTACGGTCTCAACGATAACGAAATGCAAGTG-ATGTTA 399 ** *** *** ** * * ** * ***** ***** * * * ABI3100_I-P7_004_2005-08-24 205 AACGGTTTGATGGTTTGGTGTATTGAAAATGGTACATCTCCAGACTTATC 254 ABI3100_I-M30_006_2005-08-24 400 AACGGTTTGATGGTTTGGTGTATTGAAAATGGTACATCTCCAGACTTATC 449 ** * * ********* * * ****** ****** ****** *** ABI3100_I-P7_004_2005-08-24 255 TGGTGTTTGGGTAATGATGGATGGGGAAACACAAGTTGATTATCCCATTA 304 ABI3100_I-M30_006_2005-08-24 450 TGGTGTTTGGGTAATGATGGATGGGGAAACACAAGTTGATTATCCCATTA 499 * ******************** ************** ****** *** ABI3100_I-P7_004_2005-08-24 305 AGCCTTTAATTGAACATGC AACTCCTTCGTTTAGGCAAATCATGGCTCAC 354 ABI3100_I-M30_006_2005-08-24 500 AGCCTTTAATTGAACATGC AACTCCTTCGTCCAGGCAAATCATGGCTCAC 544 ******************* ** * ** ** * * * ABI3100_I-P7_004_2005-08-24 255 TTCAGTAACGCGGCAGAGGCATACATCGCAAAGAGAAATGCCACTGAAAG 404 ABI3100_I-M30_006_2005-08-24 545 TTCAGTAACGCGGCAGAGGCATACATCGCAAAGAGAAATGCCACTGAAAG 589 *** * * * * *** * * **** * * ** **** ABI3100_I-P7_004_2005-08-24 405 GAATATGCCGCGGTATGGAATCAAGAGGAATTTGACTGACATTAGTCT 452 ABI3100_I-M30_006_2005-08-24 590 GAATATGCCGCGGTATGGAATCAAGAGGAATTTGACTGACATTAGTCT 639 ** * ** *** * * * ** ** * * ABI3100_I-P7_004_2005-08-24 453 CGCCAGAT ATGCTTTCGACTTCTATG AGGTGAATTCAAA AACAC 496 ABI3100_I-M30_006_2005-08-24 640 CGCCAGAT ATGCTTTCGACTTCTATG AGGTGAATTCAAA AACAC 689 * ** ** ** * ** ** * ** **** * * ABI3100_I-P7_004_2005-08-24 497 CTGATA-GAGCTCGTGAAGCTCATATGCAGA-TGAAGGCTGCAGCACTGC 544 ABI3100_I-M30_006_2005-08-24 690 GTGATA-GAGCTCGTGAAGCTCATATGCAGA-TGAAGGCTGCAGCACTGC 739 ** ** *** **** * * * * * ** * * * *** *** ABI3100_I-P7_004_2005-08-24 545 GTACTACTGGTCGCAGAATGTTTGGAATGGACGGCAGTGTCAGTAACAAG 594 ABI3100_I-M30_006_2005-08-24 740 GTACTACTGGTCGCAGAATGTTTGGAATGGACGGCAGTGTCAGTAACAAG 789 * * ** * *** * *** ** *** * *** * * ABI3100_I-P7_004_2005-08-24 595 GAAGAAAACACGGAGAGACACACAGTGGAAGATGTCAATAGA-GACATGC 643 ABI3100_I-M30_006_2005-08-24 790 GAAGAAAACACGGAGAGACACACAGTGGAAGATGTCAATAGA-GACATGC 839 * ******* *** *** * * * * * * * * **** ABI3100_I-P7_004_2005-08-24 644 ACTCTCTCCTGGGTATGCGCAACTGA-ATACTCGCCCATGGTTAGCTAAC 692 ABI3100_I-M30_006_2005-08-24 840 ACTCTCTCCTGGGTATGCGCAACTGA-ATACTCGCCCATGGTTAGCTAAC 899 * * ** * ***** * ** * * *** ************ 77 ABI3100_I-P7_004_2005-08-24 693 CATGGGAGAGCACTCAGTTGCGCGGCAGAGGAAGAGAGCGCATGCACAAG 730 ABI3100_I-M30_006_2005-08-24 890 CATGGGAGAGCACTCAGTTGCGCGGCAGAGGAAGAGAG 935 ****** *** * ***** ************ * *926 ABI3100_I-P7_004_2005-08-24 743 TGCCCCTGAAAGACTGTGCCCTCTCCGTGGTTTCATACAAGAAAGAGACA 792 ABI3100_I-M30_006_2005-08-24 ABI3100_I-P7_004_2005-08-24 793 AAGGCGTCCCTTCAAAAATTCTGCGACTGTATTACGCGAGCTGGAGCTTT 842 ABI3100_I-M30_006_2005-08-24 ABI3100_I-P7_004_2005-08-24 843 CTAAGGAATATGGCTCCCGAGCTAAATAGAG 873 ABI3100_I-M30_006_2005-08-24 78 Phụ lục 11 Đoạn tƣơng đồng chung cho cả 3 mẫu CLUSTAL X (1.83) multiple sequence alignment DQ Ic 1 AAAAGATAACAAAAAGAAGAAAAAGATAAACAAAAAGACAAGAATTCATGAAGGTACTAG 60 I DQ Ic 61 TGACGGAAACAATGTGTCAACTAGTACCAAAACTGGAGAGAGAGATAGAGATGTCAATGC 120 I 1 12 DQ 1 CTTTCACGGTT CCGAGAATAAAATCATTTACTGACAAGATGATTTT 46 Ic 121 CGGAACTAGTGGAACTTTCACTGTT CCGAGAATAAAATCATTTACTGACAAGATGATTTT 180 I CCGAGAATAAAATCATTTACTGACAAGATGATTTT 34 1* * * *************** *** ** ** DQ 47 ACCGAAAATTAAAGGAAAAACTGTCCTTAATTTTAAATCATCTTCTTCAGTATAATCCGC 105 Ic 181 ACCGAAAATTAAAGGAAAAACTGTCCTTAATTTTAAATCATCTTCTTCAGTATAATCCGC 239 I 35 ACCGAAAATTAAAGGAAAAACTGTCCTTAATTTTAAATCATCTTCTTCAGTATAATCCGC 94 *** ******** ******************** ************************** DQ 106 AACAAATTGATATCTCAAACACTCGTGCCACTCAAACTCAATTCGAAAAGTG-GTATGAG 164 Ic 240 AACAAATTGATATCTCAAACACTCGTGCCACTCAAACTCAATTCGAAAAGTG-GTATGAG 298 I 95 AACAAATTGATATCTCAAACACTCGTGCCACTCAAACTCAATTCGAAAAGTG-GTATGAG 153 ******************************************* ******** ***** * DQ 165 GGAGTGAGGAATGATTACGGTCTCAACGATAACGAAATGCAAGTG-ATGTTAAACGGTTT 223 Ic 299 GGAGTGAGGAATGATTACGGTCTCAACGATAACGAAATGCAAGTG-ATGTTAAACGGTTT 357 I 154 GGAGTGAGGAATGATTACGGTCTCAACGATAACGAAATGCAAGTG-ATGTTAAACGGTTT 212 ****** **************** ****** ************** ******** ***** DQ 224 GATGGTTTGGTGTATTGAAAATGGTACATCTCCAGACTTATCTGGTGTTTGGGTAATGAT 283 Ic 358 GATGGTTTGGTGTATTGAAAATGGTACATCTCCAGACTTATCTGGTGTTTGGGTAATGAT 417 I 213 GATGGTTTGGTGTATTGAAAATGGTACATCTCCAGACTTATCTGGTGTTTGGGTAATGAT 272 *************** *********************** ******************** DQ 284 GGATGGGGAAACACAAGTTGATTATCCCATTAAGCCTTTAATTGAACATGCAACTCCTTC 343 Ic 418 GGATGGGGAAACACAAGTTGATTATCCCATTAAGCCTTTAATTGAACATGCAACTCCTTC 477 I 273 GGATGGGGAAACACAAGTTGATTATCCCATTAAGCCTTTAATTGAACATGCAACTCCTTC 332 *********** ****** *********** ************************** ** DQ 344 GTTTAGGCAAATCATGGCTCACTTCAGTAACGCGGCAGAGGCATACATCGCAAAGAGGAA 402 Ic 478 GTTTAGGCAAATCATGGCTCACTTCAGTAACGCGGCAGAGGCATACATCGCAAAGAGGAA 537 I 333 GTTTAGGCAAATCATGGCTCACTTCAGTAACGCGGCAGAGGCATACATCGCAAAGAGGAA 392 *************************************************** ***** ** 404 DQ 403 TG Ic 538 TG CAACTGAAAG GTATATGCCGCGGTATGGAATCAAGAGGAATTTGACTGACATTAGC 595 I 393 TG CCACTGAAAG GAATATGCCGCGGTATGGAATCAAGAGGAATTTGACTGACATTAGT 450 ** DQ Ic 596 CTCGCTAGAT ACGCTTTCG 614 I 451 CTCGCCAGAT ATGCTTTCGACTTCTATG AGGTGAATTCAAA AACACCTGATA-G 503 DQ Ic I 504 AGCTCGTGAAGCTCATATGCAGA-TGAAGGCTGCAGCACTGCGTACTACTGGTCGCAGAA 562 DQ Ic I 563 TGTTTGGAATGGACGGCAGTGTCAGTAACAAGGAAGAAAACACGGAGAGACACACAGTGG 622 DQ Ic I 623 AAGATGTCAATAGA-GACATGCACTCTCTCCTGGGTATGCGCAACTGA-ATACTCGCCCA 680 DQ Ic I 681 TGGTTAGCTAACCATGGGAGAGCACTCAGTTGCGCGGCAGAGGAAGAGAG 730 Đoan chung cho ca ba = 393 . 69 Kết quả đọc trình tự của mồi M31 trên mẫu Mỹ Hiệp 70 Sự tƣơng đồng giữa hai mồi P14 và M31 của mẫu Mỹ Hiệp CLUSTAL X (1.83). 6 39 71 Phụ lục 9 Kết quả giải trình tự với mồi P14 của mẫu Định Quán 72 Kết quả giải trình từ với mồi M30 của Định Quán 73 Sự tƣơng đồng của hai. ACTCTCTCCTGGGTATGCGCAACTGA-ATACTCGCCCATGGTTAGCTAAC 899 * * ** * ***** * ** * * *** ************ 77 ABI3100_I-P7_004_2005-08-24 693 CATGGGAGAGCACTCAGTTGCGCGGCAGAGGAAGAGAGCGCATGCACAAG 730 ABI3100_I-M30_006_2005-08-24 890 CATGGGAGAGCACTCAGTTGCGCGGCAGAGGAAGAGAG