Preface It has been just seven years since Volumes 273 and 274 of Methods in Enzy- mology, which covered RNA polymerase and associated activities, were pub- lished. Since then, there has been an explosion in the amount of information that has come out on RNA polymerase and transcription, driven by the intensification in technology. In Volumes 370 and 371, we try to take up where Volumes 273 and 274 left off, but we include and emphasize what we feel is an important aspect of RNA polymerase that was only touched on in the previous volumes. It is quite clear now that RNA polymerase does not act alone. Its associated factors are key in determining initiation, elongation, and termin- ation occurrences. The reliance on the associated factors is often so great that it is difficult to determine whether a given protein is an RNA polymerase- associated factor or is, in fact, a subunit of the enzyme. The chapters in these volumes describe the RNA polymerase enzymes and the associated factors, and their effects on transcription initiation, elongation, and termination. The chapters expose both prokaryotic and eukaryotic systems, but they are purposely kept undivided irrespective of the origins of the RNA polymerases. We predict that as more is learned about the prokaryotic and eukaryotic systems of transcription and gene regulation, we will realize how similarly they behave rather than how differently. Sankar Adhya Susan Garges xix METHODS IN ENZYMOLOGY EDITORS-IN-CHIEF John N. Abelson Melvin I. Simon DIVISION OF BIOLOGY CALIFORNIA INSTITUTE OF TECHNOLOGY PASADENA, CALIFORNIA FOUNDING EDITORS Sidney P. Colowick and Nathan O. Kaplan Contributors to Volume 370 Article numbers are in parentheses and following the names of contributors. Affiliations listed are current. Annie-Claude Albert (11), Biology Department, Washington University in St. Louis, One Brookings Drive, St. Louis, Missouri 63130 J F. Allemand (49), Laboratoire de Phys- ique Statistique, CNRS UMR 8550, Ecole Normale Superiure, 24 rue Lho- mond, 75231 Paris, France Jennifer R. Anthony (5), Department of Bacteriology, University of Wisconsin – Madison, 1550 Linden Drive, Madison, Wisconsin 53706 Larry C. Anthony (16), McArdle Labora- tory of Cancer Research, University of Wisconsin, 1400 University Avenue, Madison, Wisconsin 53706 Jae-Bum Bae (7), Laboratory of Molecular Microbiology, School of Biological Sci- ences, Seoul National University, Seoul 151-742, Korea Vladimir B. Bajic (21), Laboratories for Information Technology, 21, Heng Mui Keng Terrace, Singapore 119613 Rajiv P. Bandwar (55), Department Biochemistry, UMDNJ, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854 Peter B. Becker (42), Adolf-Butenandt- Institut, Molekularbiologie, Ludwig- Maximilians-Universitat, Schillerstrasse 44, D80336 Munchen, Germany Veit Bergendahl (17), McArdle Labora- tory of Cancer Research, University of Wisconsin, 1400 University Avenue, Madison, Wisconsin 53706 Sukesh R. Bhaumik (38), Department of Biochemistry and Molecular Biology, Southern Illinois University, Carbondale, Illinois 62901 Vladimir Bondarenko (29), Department of Biochemistry and Molecular Biology, Wayne State University School of Medi- cine, Detroit, Michigan 48201 Patricia Bordes (53), Department of Biological Sciences, National College of Science, Technology, and Medicine, SAFB, Imperial College Road, London SW7 2AZ, United Kingdom Robert Britton (23), Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824 Vladimir Brusic (21), Laboratories for Information Technology, 21, Heng Mui Keng Terrace, Singapore 119613 Martin Buck (3, 53), Department of Bio- logical Sciences, Wolfson Laboratories, Imperial College of London, Rm. 313, London SW7 2AY, United Kingdom Richard Burgess (16, 17), McArdle Laboratory of Cancer Research, Univer- sity of Wisconsin, 1400 University Avenue, Madison, Wisconsin 53706 Patricia C. Burrows (3), Department of Biological Sciences, Wolfson Laborator- ies, Imperial College of London, Rm. 313, London SW7 2AY, United Kingdom Julio Cabrera (1), Laboratory of Molecu- lar Biology, National Cancer Institute, National Institutes of Health, Bldg. 37, Rm. 5144, Bethesda, Maryland 20892 xi Wendy Cannon (53), Department of Biological Sciences, National College of Science, Technology, and Medicine, SAFB, Imperial College Road, London SW7 2AZ, United Kingdom Michael W. Capp (45), Department of Chemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706-1544 Frederic Coin (58), Institut de Genetique et de Biologie Moleculaire et Cellulaire, Dept. of Transcription, CNRS/INSERM/ ULP, B.P. 162, 67404 Illkirch Cedex, C.U. de Strasbourg, France Ronald C. Conaway (59), Dept. of Bio- chemistry and Molecular Biology, Kansas University Medical Center, Kansas City, Missouri 66160 Benoit Coulombe (57), Laboratory of Gene Transcription, Clinical Research In- stitute of Montreal, 110 Pine Avenue West, Montreal, Quebec H2W 1R7, Canada V. Croquette (49), Laboratoire de Phys- ique Statistique, CNRS UMR 8550, Ecole Normale Superiure, 24 rue Lho- mond, 75231 Paris, France Michael E. Dahmus (13), Section of Mo- lecular and Cell Biology, Division of Bio- logical Science, University of California, Davis, Davis, California 95616-5224 Seth A. Darst (4), The Rockefeller Univer- sity, 1230 York Avenue, Box 224, New York, New York 10021 Dipak Dasgupta (50), Biophysics Division, Saha Institute of Nuclear Physics, 37, Belgachia Road, Calcutta 700037, India Margaret A. Daugherty (31), Depart- ment of Biochemistry, University of Ver- mont College of Medicine, Burlington, Vermont 05405 Elena K. Davydova (8), Department of Molecular Genetics and Cell Biology, University of Chicago, 920 E. 58th Street, Chicago, Illinois 60637 Pieter deHaseth (47), Department of Bio- chemistry, Case Western Reserve University, Cleveland, Ohio 44106-4935 Borries Demeler (43), Center for Analyt- ical Ultracentrifugation of Macromolecu- lar Assemblies, University of Texas Health Science Center, San Antonio, Texas Jonathan A. Dodd (10), Department of Biological Chemistry, University of Cali- fornia – Irvine, 240 D Medical Sciences I, Irvine, California 92697-1700 Timothy J. Donohue (5), Department of Bacteriology, University of Wisconsin – Madison, 1550 Linden Drive, Madison, Wisconsin 53706 David Dunlap (32), DIBIT 3A3, san Raf- faele Scientific Institute, via Olgettina, 58, Milan 20132, Italy Arik Dvir (59), Department of Biological Sciences, Oakland University, Rochester, Michigan 48309 Keith Earley (11), Biology Department, Washington University in St. Louis, One Brookings Drive, St. Louis, Missouri 63130 R. H. Ebright (49), Howard Hughes Med- ical Institute, Waksman Institute, De- partment of Chemistry, Rutgers University, Piscataway, New York 08854 Jean Marc Egly (58), Institut de Genetique et de Biologie Moleculaire et Cellulaire, Department of Transcription, CNRS/IN- SERM/ULP, B.P. 162, 67404 Illkirch Cedex, C.U. de Strasbourg, France Robert D. Finn (3), The Wellcome Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom xii contributors to volume 370 Laura Finzi (32), Dip Biol ‘‘Luigi Gorini,’’ U Degli Studi Milano, Via Celoria 26, Milan I-20133 Italy Katherine M. Foley (16), McArdle Lab- oratory of Cancer Research, University of Wisconsin, 1400 University Avenue, Madison, Wisconsin 53706 Diane Forget (57), Laboratory of Gene Transcription, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, Quebec H2W 1R7, Canada Michael G. Fried (31), Department of Biochemistry and Molecular Biology, The Pennsylvania State University Col- lege of Medicine, 500 University Drive, Hershey, Pennsylvania 17033 Susan Garges (25), Laboratory of Mo- lecular Biology, CCR, National Cancer Institute, National Institutes of Health, Bldg. 37, Rm. 5138, Bethesda, Maryland 20892 S. Gilfillan (40), GSF-National Research Center for Environment and Health, De- partment of Gene Expression, Institute of Molecular Immunology, Marchionini-str. 25, Munich D-81377, Germany James A. Goodrich (56), Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, Color- ado 80309-0215 Richard L. Gourse (51), Department of Bacteriology, University of Wisconsin – Madison, 1550 Linden Drive, Madison, Wisconsin 53706-1567 Brent Gowen (3), 627 Gower Point Road, Gibson’s Landing, British Columbia V0N 1V8, Canada Heather A. Green (5), Department of Bacteriology, University of Wisconsin – Madison, 1550 Linden Drive, Madison, Wisconsin 53706 Michael R. Green (36, 38), Program in Gene Function and Expression, 364 Plan- tation Street, Worcester, Massachusetts 01605-2377 Jack Greenblatt (34), Banting and Best Department of Medical Research, Univer- sity of Toronto, Toronto, Ontario M5G 1L6, Canada Carol A. Gross (18), Departments of Sto- matology and Microbiology and Immu- nalogy, University of California, San Francisco, California 94143-0512 Tanja M. Gruber (18), Departments of Stomatology and Microbiology and Im- munalogy, University of California, San Francisco, California 94143-0512 Mi-Young Hahn (7), Laboratory of Molecular Microbiology, School of Biological Sciences, Seoul National Uni- versity, Seoul 151-742, Korea Mitsuhiro Hamada (14), Department of Integrated Biosciences, University of Tokyo, Tokyo, Japan John D. Helmann (2), Department of Microbiology, Cornell University, Ithaca, New York 14853 Susanne Hoheisel (34), Department of Molecular and Cell Biology, Division of Biochemistry and Molecular Biology, 401 Barker Hall, Berkeley, Californaia 94720-3202 Ying Huang (14), Laboratory of Molecu- lar Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-2753 Diane Imburgio (19), Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, 190 Frelinghuysen Road, Piscataway, New Jersey 08854 contributors to volume 370 xiii Ding Jun Jin (1, 15, 25, 26), Laboratory of Molecular Biology, National Cancer In- stitute, National Institutes of Health, Bldg. 37, Rm. 5144, Bethesda, Maryland 20892 Susan Jones (53), Department of Bio- logical Sciences, National College of Sci- ence, Technology, and Medicine, SAFB, Imperial College Road, London SW7 2AZ, United Kingdom Caroline M. Kane (34), Department of Molecular and Cell Biology, Division of Biochemistry and Molecular Biology, 401 Barker Hall, Berkeley, California 94720- 3202 Changwon Kang (54), Department of Bio- logical Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejon 305-701, Republic of Korea Mikhail Kashlev (12), NCI Center for Cancer Research, National Cancer Insti- tute, Frederick Cancer Research and Development Center, Bldg. 539, Rm. 222, Frederick, Maryland 21702 Krystyna M. Kazmierczak (8), Depart- ment of Molecular Genetics and Cell Biol- ogy, University of Chicago, 920 E. 58th Street, Chicago, Illinois 60637 Patricia J. Kiley (27), Department of Bio- molecular Chemistry, University of Wis- consin, 574 Medical Science Center, 1300 University Avenue, Madison, Wisconsin 53706 Maria L. Kireeva (12), NCI Center for Cancer Research, National Cancer Insti- tute, Frederick Cancer Research and De- velopment Center, Bldg. 539, Rm. 222, Frederick, Maryland 21702 Bruno P. Klaholz (3), Institut de Genet- ique et de Biologie Moleulaire et Celle- laire, Dept. de Biologie et Genomique Structurales, 1, rue Laurent Fries, 67404 Illkirch, France Michael S. Kobor (34), Department of Molecular and Cell Biology, Division of Biochemistry and Molecular Biology, 401 Barker Hall, Berkeley, California 94720- 3202 Natalia Komissarova (12), NCI Center for Cancer Research, National Cancer Institute, Frederick Cancer Research and Development Center, Bldg. 539, Rm. 222, Frederick, Maryland 21702 E. Kremmer (40), GSF-National Research Center for Environment and Health, De- partment of Gene Expression, Institute of Molecular Immunology, Marchionini-str. 25, Munich D-81377, Germany Jennifer F. Kugel (56), Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, Color- ado 80319-0215 Konstantin Kuznedelov (9), Limnol- ogical Institute of the Russian Academy of Sciences, Irkutsk, Russia Lester J. Lambert (43), Laboratory of Molecular Biophysics, The Rockefeller University, New York, New York 10021 Dale E. A. Lewis (52), Laboratory of Mo- lecular Biology, National Cancer Insti- tute, 37 Convent Drive, Rm. 5138, Bethesda, Maryland 20892-4255 Patrick S. Lin (13), Section of Molecular and Cell Biology, Division of Biological Science, University of California, Davis, Davis, California 95616-5224 Ye V. Liu (29), Department of Biochemis- try and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201 Lucyna Lubkowska (12), NCI Center for Cancer Research, National Cancer Insti- tute, Frederick Cancer Research and De- velopment Center, Bldg. 539, Rm. 222, Frederick, Maryland 21702 xiv contributors to volume 370 Betina Macho (35), Institut de Genetique et de Biologie Moleculaire et Cellulaire, 1, rue Laurent Fries, 67404 Illkirch, Strasbourg, France Richard Maraia (14), Laboratory of Mo- lecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-2753 Robert G. Martin (24), Laboratory of Molecular Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892-0560 Shoko Masuda (4), The Rockefeller Uni- versity, 1230 York Avenue, Box 224, New York, New York 10021 Kalai Mathee (22), Department of Bio- logical Sciences, Florida International University, Miami, Florida 33199 Walter Messer (30), Max-Planck-Insti- tute for Molecular Genitics, D-14195 Berlin, Germany M. Miesterernst (40), GSF-National Re- search Center for Environment and Health, Department of Gene Expression, Institute of Molecular Immunology, Marchionini-str. 25, Munich D-81377, Germany Leonid Minakhin (9), Waksman Institute of Microbiology, Rutgers, The State Uni- versity of New Jersey, 190 Frelinghuysen Road, Piscataway, New Jersey 08854 Aaron P. Mitchell (41), Department of Microbiology and Institute of Cancer Re- search, Columbia University, 701 West 168th Street, New York, New York 10032 Katsuhiko S. Murakami (4), The Rocke- feller University, 1230 York Avenue, Box 224, New York, New York 10021 Heath D. Murray (51), Department of Bacteriology, University of Wisconsin – Madison, 1550 Linden Drive, Madison, Wisconsin 53706-1567 Yoshihiro Nakatani (37), Dana-Farber Cancer Institute and Harvard Medical School, Boston, Maryland 02115 Giri Narasimhan (22), School of Com- puter Science, ECS 389, Florida Inter- national University, Miami, Florida 33199 Sergei Nechaev (19, 53), CMG, University of California San Diego, 9500 Gilman Drive, La Jolla, California 92093 Yur A. Nedialkov (44), Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824-1319 Alexander J. Ninfa (29), Department of Biochemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109-0606 Masayasu Nomura (10), Department of Biological Chemistry, University of Cali- fornia – Irvine, 240 D Medical Sciences I, Irvine, California 92697-1700 Michael C. O’Neill (20), Department of Biological Sciences, University of Mary- land, Baltimore County (UMBC), 1000 Hilltop Circle, Baltimore, Maryland 21250 Vasily Ogryzko (37), Laboratoire Onco- genese, Differenciation et Transduction du Signal, CNRS UPR 9079, Institut Andre Lwoff, Villejuif, France Elena V. Orlova (3), Department of Crys- tallography, Birbeck College, Malet Street, London WC1E 7HX, United Kingdom Joo-Hong Park (7), Laboratory of Molecular Microbiology, School of Biological Sciences, Seoul National Uni- versity, Seoul 151-742, Korea Smita S. Patel (55), Department Biochem- istry, UMDNJ, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscat- away, New Jersey 08854 contributors to volume 370 xv Erik Pierstorff (34), Department of Molecular and Cell Biology, Division of Biochemistry and Molecular Biology, 401 Barker Hall, Berkeley, California 94720- 3202 Craig S. Pikaard (11), Biology Depart- ment, Washington University in St. Louis, One Brookings Drive, St. Louis, Missouri 63130 Aparna Raval (33), Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bldg. 10, Rm. 4B36, Bethesda, Maryland 20892-1360 Pampa Ray (3), Department of Biological Sciences, Wolfson Laboratories, Imperial College of London, Rm. 313, London SW7 2AY, United Kingdom M. Thomas Record Jr. (45), Department of Chemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706-1544 Joseph C. Reese (36), Deptartment of Bio- chemistry and Molecular Biology, Penn- sylvania State University, 203 Althouse Laboratory, University Park, Pennsylva- nia 16802 John N. Reeve (6), Department of Microbiology, Ohio State University, Columbus, Ohio 43210 A. Revyakin (49), Howard Hughes Med- ical Institute, Waksman Institute, Depart- ment of Chemistry, Rutgers University, Piscataway, New York 08854 Vikas Rishi (39), Laboratory of Metabol- ism, National Cancer Institute, National Institutes of Health, Bldg. 37, Rm. 2D24, Bethesda, Maryland 20892 Jung-Hye Roe (7), Laboratory of Molecu- lar Microbiology, School of Biological Sciences, Seoul National University, Seoul 151-742, Korea Judah L. Rosner (24), Laboratory of Mo- lecular Biology, NIDDK, National Insti- tutes of Health, Bethesda, Maryland 20892-0560 Lucia B. Rothman-Denes (8), Depart- ment of Molecular Genetics and Cell Biol- ogy, University of Chicago, 920 E. 58th Street, Chicago, Illinois 60637 Siddhartha Roy (46, 48), Department of Biophysics, Bose Institute, P-1/12, C.I.T., Scheme VII M, Calcutta 700 054, India Ruth M. Saecker (45), Department of Chemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706-1544 Julio Saez-Vasquez (11), Biology Depart- ment, Washington University in St. Louis, One Brookings Drive, St. Louis, Missouri 63130 Raphael Sandaltzopoulos (42), Depart- ment of Molecular Biology, Democritus University of Thrace, University Hospital at Dragana, G-68100 Alexandroupolis, Greece Paola Sassone-Corsi (35), Institut de Ge- netique et de Biologie Moleculaire et Cel- lulaire, 1, Rue Laurent Fries, 67404 Illkirch, Strasbourg, France Virgil Schirf (43), Center for Analytical Ultracentrifugation of Macromolecular Assemblies, University of Texas Health Science Center, San Antonio, Texas David A. Schneider (51), Department of Bacteriology, University of Wisconsin – Madison, 1550 Linden Drive, Madison, Wisconsin 53706-1567 Ranjan Sen (50), Laboratory of Transcrip- tion Biology, Center for DNA Finger- printing and Diagnosis, ECIL Road, Nacharam, Hyderabad 500076, India Steve D. Seredick (28), Department of Microbiology and Immunology, Univer- sity of British Columbia, 6174 University Boulevard, Vancouver, British Columbia V6T 123, Canada Konstantin Severinov (9, 19, 53), Depart- ment of Molecular Biology and Biochem- istry, Rutgers, The State University of New Jersey, 190 Frelinghuysen Road, Piscataway, New Jersey 08854 xvi contributors to volume 370 Mitsuhiro Shimizu (41), Deptartment of Chemistry, Meisei University, Tokyo 191-8506, Japan Inkyung Shin (54), Department of Bio- logical Sciences, Korea Advanced Insti- tute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejon 305- 701, Republic of Korea Dean D. Shooltz (44), Department of Bio- chemistry and Molecular Biology, Mich- igan State University, East Lansing, Michigan 48824-1319 Dinah S. Singer (33), Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bldg. 10, Rm. 4B36, Bethesda, Maryland 20892-1360 George B. Spiegelman (28), Department of Microbiology and Immunology, Uni- versity of British Columbia, 6174 Univer- sity Boulevard, Vancouver, British Columbia V6T 123, Canada G. Stelzer (40), GSF-National Research Center for Environment and Health, De- partment of Gene Expression, Institute of Molecular Immunology, Marchionini-str. 25, Munich D-81377, Germany T. R. Strick (49), Cold Spring Harbor Lab- oratory, Cold Spring Harbor, New York 11724 Vasily M. Studitsky (29), Department of Biochemistry and Molecular Biology, Wayne State University School of Medi- cine, Detroit, Michigan 48201 Maxim V. Sukhodolets (25), Developmen- tal Genetics Section, Laboratory of Mo- lecular Biology, CCR, National Cancer Institute, National Institutes of Health, Bldg. 37, Rm. 5138 Bethesda, Maryland 20892 Victoria Sutton (27), Department of Bio- molecular Chemistry, University of Wis- consin, 574 Medical Science Center, 1300 University Avenue, Madison, Wisconsin 53706 Nancy E. Thompson (16), McArdle Lab- oratory of Cancer Research, University of Wisconsin, 1400 University Avenue, Madison, Wisconsin 53706 Prasad Tongaonkar (10), Department of Biological Chemistry, University of Cali- fornia – Irvine, 240 D Medical Sciences I, Irvine, California 92697-1700 Steven J. Triezenberg (44), Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824-1319 Oleg V. Tsodikov (45), Harvard Medical School, Boston, Massachusetts 02115 Laura Tsujikawa (47), Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106-4935 Barb M. Turner (28), Department of Microbiology and Immunology, Univer- sity of British Columbia, 6174 University Boulevard, Vancouver, British Columbia V6T 123, Canada Marin van Heel (3), Department of Biological Sciences, Wolfson Laborator- ies, Imperial College of London, Rm. 313, London SW7 2AY, United Kingdom Charles Vinson (39), Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Building 37, Rm. 2D24, Bethesda, Maryland 20892 Christoph Weigel (30), Max-Planck- Institute for Molecular Genitics, D-14195 Berlin, Germany Jocelyn D. Weissman (33), Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bldg. 10, Rm. 4B36, Bethesda, Maryland 20892-1360 Joan Weliky Conaway (59), Stowers In- stitute of Medical Research, 1000 E. 50th Street, Kansas City, Missouri 64110 contributors to volume 370 xvii Milton H. Werner (43), Laboratory of Molecular Biophysics, The Rockefeller University, New York, New York 10021 Siva R. Wigneshweraraj (53), Depart- ment of Biological Sciences, National College of Science, Technology, and Medicine, SAFB, Imperial College Road, London SW7 2AZ, United Kingdom Yunwei Xie (6), Department of Microbiol- ogy, Ohio State University, Columbus, Ohio 43210 Wenxue Yang (26), Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bldg. 37, Rm. 5144, Bethesda, Maryland 20892 Huijun Zhi (15, 26), Laboratory of Mo- lecular Biology, National Cancer Insti- tute, National Institutes of Health, Bldg. 37, Rm. 5144, Bethesda, Maryland 20892 xviii contributors to volume 370 [...]... Genetics (Part B) Edited by Chandan K Sen and Lester Packer Volume 354 Enzyme Kinetics and Mechanisms (Part F: Detection and Characterization of Enzyme Reaction Intermediates) Edited by Daniel L Purich Volume 355 Cumulative Subject Index Volumes 321–354 Volume 356 Laser Capture Microscopy and Microdissection Edited by P Michael Conn Volume 357 Cytochrome P450, Part C Edited by Eric F Johnson and Michael... Bioluminescence and Chemiluminescence (Part B) Edited by Marlene DeLuca and William D McElroy Volume 134 Structural and Contractile Proteins (Part C: The Contractile Apparatus and the Cytoskeleton) Edited by Richard B Vallee Volume 135 Immobilized Enzymes and Cells (Part B) Edited by Klaus Mosbach Volume 136 Immobilized Enzymes and Cells (Part C) Edited by Klaus Mosbach Volume 137 Immobilized Enzymes and Cells... Biofilms (Part B: Special Environments and Physicochemical Aspects) Edited by Ron J Doyle Volume 338 Nuclear Magnetic Resonance of Biological Macromolecules (Part A) ¨ Edited by Thomas L James, Volker Dotsch, and Uli Schmitz Volume 339 Nuclear Magnetic Resonance of Biological Macromolecules (Part B) ¨ Edited by Thomas L James, Volker Dotsch, and Uli Schmitz Volume 340 Drug–Nucleic Acid Interactions Edited... Separation and Analysis of Biological Macromolecules (Part B: Applications) Edited by Barry L Karger and William S Hancock Volume 272 Cytochrome P450 (Part B) Edited by Eric F Johnson and Michael R Waterman Volume 273 RNA Polymerase and Associated Factors (Part A) Edited by Sankar Adhya Volume 274 RNA Polymerase and Associated Factors (Part B) Edited by Sankar Adhya Volume 275 Viral Polymerases and Related... 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Hormone Action (Part. Lubkowska (12), NCI Center for Cancer Research, National Cancer Insti- tute, Frederick Cancer Research and De- velopment Center, Bldg. 539, Rm. 222, Frederick, Maryland 21702 xiv contributors to. Frederick Cancer Research and Development Center, Bldg. 539, Rm. 222, Frederick, Maryland 21702 Krystyna M. Kazmierczak (8), Depart- ment of Molecular Genetics and Cell Biol- ogy, University of Chicago,