Engineering triterpene production in Saccharomyces cerevisiae – b-amyrin synthase from Artemisia annua James Kirby 1 , Dante W. Romanini 2 , Eric M. Paradise 1,3 and Jay D. Keasling 1,3,4,5 1 California Institute for Quantitative Biomedical Research, University of California, Berkeley, CA, USA 2 Department of Chemistry, University of California, Berkeley, CA, USA 3 Department of Chemical Engineering, University of California, Berkeley, CA, USA 4 Department of Bioengineering, University of California, Berkeley, CA, USA 5 Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA Triterpenes belong to the isoprenoid family of com- pounds and are recognized by their C 30 backbones. They are typically synthesized by the cyclization of the sterol precursor 2,3-oxidosqualene into a multi-ringed compound with a single alcohol group. Fungi and mammals convert 2,3-oxidosqualene to the triterpene compound lanosterol in the biosynthetic pathways to ergosterol and cholesterol, respectively. The equivalent step in plant primary metabolism is the cyclization of 2,3-oxidosqualene to cycloartenol for the production of membrane sterols. Cycloartenol is also the triter- pene precursor of brassinosteroid phytohormones that regulate plant growth and development [1,2]. Based on chemical and genetic analyses performed to date, it appears that plants are more diverse than animals or fungi in the range of tritepene products synthesized [3]. However, despite the fact that a large variety of triter- pene compounds have been isolated from plant sources [4], the majority of triterpene synthase genes isolated to date have encoded either lupeol or b-amyrin synth- ases (EC 5.4.99.–) [1]. b-amyrin in particular serves as the olefin precursor to a wide range of downstream products. The action of oxidative enzymes (typically cytochrome P450 monooxygenases) and glyco- syltransferases convert b-amyrin to various triterpene saponins in different plant species [5–7]. These sapo- nins may perform protective roles in the host plant, acting as antimicrobial [8] and insecticidal [9] agents, and many of these compounds are also of interest from a human health perspective. The effect of plant saponins on low-density lipoprotrein cholesterol absorption and arterial atherosclerosis has received much attention, leading to the development of several cholesterol-reducing dietary supplements [10]. Saponins Keywords Artemisia annua; isoprenoids; metabolic engineering; Saccharomyces cerevisiae; b-amyrin synthase Correspondence J. D. Keasling, Berkeley Center for Synthetic Biology, 717 Potter Street, Building 977, Mail code 3224, University of California, Berkeley, CA 94720-3224, USA Fax: +1 510 495 2630 Tel: +1 510 495 2620 E-mail: keasling@berkeley.edu (Received 12 December 2007, revised 11 February 2008, accepted 18 February 2008) doi:10.1111/j.1742-4658.2008.06343.x Using a degenerate primer designed from triterpene synthase sequences, we have isolated a new gene from the medicinal plant Artemisia annua. The predicted protein is highly similar to b-amyrin synthases (EC 5.4.99.–), sharing amino acid sequence identities of up to 86%. Expression of the gene, designated AaBAS,inSaccharomyces cerevisiae, followed by GC ⁄ MS analysis, confirmed the encoded enzyme as a b-amyrin synthase. Through engineering the sterol pathway in S. cerevisiae, we explore strategies for increasing triterpene production, using AaBAS as a test case. By manipula- tion of two key enzymes in the pathway, 3-hydroxy-3-methylglutaryl-CoA reductase and lanosterol synthase, we have improved b-amyrin production by 50%, achieving levels of 6 mgÆL )1 culture. As we have observed a 12-fold increase in squalene levels, it appears that this strain has the capa- city for even higher b-amyrin production. Options for further engineering efforts are explored. Abbreviation HMGR, HMG-CoA reductase. 1852 FEBS Journal 275 (2008) 1852–1859 ª 2008 The Authors Journal compilation ª 2008 FEBS may also find applications in ruminant nutrition [11], as anticancer agents [12,13], and as vaccine adjuvants [14]. Although triterpene synthases have been expressed in microbial hosts such as Saccharomyces cerevisiae, there has been little effort made so far to engineer the metabolism of a microbial host for enhanced produc- tion of triterpenes. By contrast, there have been many considerable efforts to engineer microbes for higher production of mono-, sesqi- and diterpenes [15]. These projects have mainly focused on the overexpression of enzymes involved in either of the two pathways (mevalonate or 1-deoxy-d-xylulose-5-phosphate) res- ponsible for the biosynthesis of isoprenoids [16–18]. In S. cerevisiae, the mevalonate pathway is responsible for the biosynthesis of isoprenoids and sterols. A good deal is known about regulatory mechanisms within the pathway, although the majority of studies have focused on the upper part of the pathway, from acetyl- CoA to squalene. Our knowledge of how the lower half of the pathway, from squalene to ergosterol, is regulated remains somewhat limited. As the branch point for triterpene biosynthesis is located in this latter half of the pathway, the optimal steps to increase their production in yeast are not immediately apparent. Artemisia annua, or sweet wormwood, has been used medicinally for centuries, predominantly in China [19]. A sesquiterpene constituent, artemisinin, is one of the most important drugs used in the treatment of malaria. In an effort to isolate and characterize new terpene synthases from A. annua, we have designed degenerate primers for use in RT-PCR. Here, we describe the isolation of a b-amyrin synthase gene from A. annua and its expression in S. cerevisiae. Our findings on engineering overproduction of b-amyrin in S. cerevisiae should be relevant to the production of any triterpene. Results Isolation and verification of a b-amyrin synthase In order to isolate new triterpene synthase genes from A. annua, several degenerate primers were designed from an alignment of plant triterpene synthase protein sequences. 3¢ RACE reactions were carried out on RNA isolated from A. annua leaf tissue, and a product of the expected size was obtained with the primer TriF1 (Table 1). The fragment was cloned and the sequence was found to be homologous to triterpene synthase genes. Gene-specific primers were designed for 5¢ RACE, and a product was obtained that contained the likely start codon, based on protein sequence alignments, with 175 nt of the upstream 5¢ UTR sequence. The predicted full-length gene encodes a 762 amino acid protein that shares over 70% identity with plant b-amyrin synthases. The most closely related protein (AAX14716), sharing 86% sequence identity, is the b-amyrin synthase from Aster sedifolius, a plant which belongs to the asteroi- deae, the same sub-family as A. annua (Fig. 1). Published detection methods for triterpenes such as b-amyrin are generally laborious, and are inconvenient when processing a large number of samples [5,20]. Therefore, we attempted to streamline the process by eliminating the sample clean-up steps normally per- formed after cell extraction, or the need for derivatiza- tion. Cell disruption and saponification was performed as previously described, using a mixture of EtOH and KOH [20]. We found that using the nonpolar solvent dodecane for extraction allowed us to follow this directly with separation by GC ⁄ MS, using the highest possible temperature settings for the mass spectrometer ion source and quadrupole. This proved to be a sensi- tive and robust method for the detection of b-amyrin and the cell sterol components squalene and ergo- sterol. The coding sequence of the gene, designated AaBAS, was cloned into the high-copy yeast expression vector pESC-URA, under control of the GAL10 promoter, and transformed into S. cerevisiae to create the strain bamy1. After induction of AaBAS expression with galactose, sterols were extracted from cells and ana- lyzed by GC ⁄ MS. A single chromatographic peak was found in extracts from bamy1 cells that was absent in cells containing an empty vector. The retention time of this compound was identical to that of a b-amyrin standard, and the corresponding mass spectra were found to match (Fig. 2). An in vitro assay using a bamy1 cell extract and the triterpene substrate 2,3- oxidosqualene was also found to result in production Table 1. Oligonucleotides used. The restriction sites used in clon- ing are underlined. Oligonucleotide Sequence (5¢-to3¢) TriF1 ATGYTNGCNTGYTGGRTNGARGAYCC PolyT-anchor GAGCTCGAGATCTAAGCTTGCTTTTTTTTTTTTTT TTTTTT Anchor GAGCTCGAGATCTAAGCTTGC TriRaceR1 GATCCTGCTGGTTCCCATGCGGCTA TricdsF AAC GAATTCAACAATGTGGAGATTGAAAATAGCAG AAGGGCGCAATG TricdsR AAC GAGCTCCTAGGTGCCTTTGAGCTGTGGCAGCA CCTGCTTG ERG7F TAC CCATGGCAGAATTTTATTCTGACACAATCGGTC ERG7R CC ATCGATCCATCAACCGGATGTGCTGTATTGACG J. Kirby et al. Engineering triterpene production in yeast FEBS Journal 275 (2008) 1852–1859 ª 2008 The Authors Journal compilation ª 2008 FEBS 1853 of the b-amyrin product. The product was not observed in the absence of either the substrate or the AaBAS gene (data not shown). Production of b-amyrin in engineered strains of S. cerevisiae We attempted to increase production of b-amyrin by modifying flux through the sterol biosynthetic pathway of S. cerevisiae. The enzyme 3-hydroxy-3-methylgluta- ryl-CoA reductase (HMGR, represented by two iso- zymes: HMG1 and HMG2; Fig. 3) is known to act as a control point in the sterol pathway, with one of the primary control mechanisms being degradation of the HMGR protein in response to accumulation of the squalene precursor farnesyl pyrophosphate [21,22]. Expression of a truncated form of the HMG1 (tHMG1) protein circumvents this feedback, which occurs via the N-terminal transmembrane domain of the enzyme [23]. Furthermore, expression of tHMG1 from an independently-regulated promoter will bypass any transcriptional control of expression. Strain bamy2 contains an integrated copy of tHMG1 under control of the GAL1 promoter in addition to pESC-AaBAS. In agreement with previous studies [23,24], bamy2 accumulated significantly higher levels of squalene compared to bamy1 (Fig. 4A). However, this eight-fold increase in squalene did not translate into increased yields of b-amyrin; rather the b-amyrin levels from bamy2 were one-third of that produced by bamy1 (Fig. 4C). The ergosterol content of the cells remained essentially unchanged in strain bamy2 (Fig. 4B), which is consistent with the view that there is a feedback con- trol mechanism in the pathway between squalene and ergosterol [23]. bamy2 grew extremely slowly at first (Fig. 4D), as observed previously [23], where it was attributed to accumulation of toxic intermediates such as farnesyl pyrophosphate. In a separate approach, we tested whether downre- gulation of lanosterol synthase (ERG7; Fig. 3) would provide more 2,3-oxidosqualene substrate for AaBAS. Other studies have shown that triterpene production can be enhanced by deleting ERG7 completely [20]. However, as erg7 strains require feeding with ergo- sterol, this approach is economically limited for indus- trial purposes. To enable the downregulation of ERG7, we modified strain bamy1 by replacing the native ERG7 promoter with the methionine-repressible pro- moter of the MET3 gene [22]. Thus, the strain bamy3 contains a P MET3 -ERG7 replacement of the native ERG7 gene in addition to pESC-AaBAS. Following downregulation of ERG7, strain bamy3 was found to Fig. 1. Pairwise sequence alignment of AaBAS from A. annua (upper line) with its closest known relative, b-amyrin synthase from A. sedifo- lius (AAX14716, lower line). The position of the primer TriF1 is indicated by the arrow. Engineering triterpene production in yeast J. Kirby et al. 1854 FEBS Journal 275 (2008) 1852–1859 ª 2008 The Authors Journal compilation ª 2008 FEBS accumulate similar levels of squalene to strain bamy1, whereas b-amyrin levels were slightly higher (Fig. 4). It is interesting to note that ergosterol levels in the cell were not reduced in response to ERG7 limitation. Veen et al. [25] have shown that, when squalene epoxi- dase (ERG1) is overexpressed, there is no accumula- tion of 2,3-oxidosqualene, but rather of lanosterol, indicating that ERG7 is not a flux-limiting enzyme. In addition, it is likely that regulation of the pathway was adjusted in response to the reduced ERG7 transcript levels in order to maintain ergosterol production. Indeed, it was necessary to optimize the relative timing of AaBAS induction and ERG7 repression to even maintain the same b-amyrin production levels as strain bamy1. When induction and repression were simulta- neous, b-amyrin production levels were actually lower in strain bamy3 and, thus, it was necessary to delay repression of ERG7 until 24–48 h after AaBAS induc- tion in order to allow AaBAS to first accumulate (data not shown). The data shown in the present study were generated by inducing AaBAS at inoculation and repressing ERG7 43 h later by the addition of 1 mm methionine. We next decided to combine the two strategies in order to test whether the feedback regulation that appears to take place in the tHMG1 strain bamy2 may be overcome by downregulating ERG7. Strain bamy4 was therefore constructed from strain bamy2 by replacing the native ERG7 promoter with the MET3 promoter. Again, it was found that optimal results were obtained when ERG7 was repressed by the addi- tion of methionine 24–48 h after induction of AaBAS expression. bamy4 did not exhibit the same growth lag phase observed in bamy2, and grew at approximately the same rate as bamy1 (Fig. 4D). Interestingly, squa- lene accumulated in b amy4 to even greater levels than Time (min) Total ion abundance A BY4742 wt strain AaBAS in vivo product β-amyrin standard β-amyrin standard HO AaBAS in vivo product % % m/ z m/ z B Fig. 2. Confirmation of b-amyrin production in S. cerevisiae by expression of AaBAS. (A) Overlaid GC ⁄ MS chromatographs of extracts from strains BY4742 and bamy1 with an authentic b-amyrin standard (with b-amyrin structure shown) (B) Mass spectra extracted from the peaks shown in (A). J. Kirby et al. Engineering triterpene production in yeast FEBS Journal 275 (2008) 1852–1859 ª 2008 The Authors Journal compilation ª 2008 FEBS 1855 those found in bamy2, corresponding to a 12-fold increase over those in bamy1 (Fig. 4A). In this case, however, b-amyrin production levels were also signifi- cantly enhanced, resulting in a 50% increase in yield over bamy1 (Fig. 4C). Discussion We have shown that it is possible to engineer increased production of tritepenes in S. cerevisiae without the need for feeding with exogenous sterols. The 50% increase in b-amyrin levels demonstrated in the present study should be considered in the light of the fact that triterpene production may not be as amenable to engi- neering efforts as the volatile sesquiterpenes and mono- terpenes that readily diffuse out of the cell. However, it is apparent that further progress can be made and there are some clues as to what these next steps may comprise. We have achieved a 12-fold increase in squa- lene levels over the initial bamy1 strain, and a logical course of action would be to find a way to convert this squalene into b-amyrin. M’baya et al. [26] demonstrated that ERG1 activity is reduced in the presence of excess sterols through a mechanism other than enzyme inhibition, most likely transcriptional repression. Not a great deal is known about how the latter half of the sterol pathway is regu- lated and exactly what role ERG1 plays in this pro- cess. However, the fact that we observe a further increase in squalene upon downregulation of ERG7 in strain bamy4 would indicate that 2,3-oxidosqualene can act as a repressor of ERG1. Tight regulation at ERG1 would make sense as it marks the beginning of the oxygen-dependent reactions in the pathway. If the feedback regulation is transcriptional in nature, then it Acetyl-CoA HMG-CoA Mevalonate HMG1,2 Squalene 2,3-oxidosqualene ERG1 Lanosterol ERG7 β-amyrin AaBAS Ergosterol Fig. 3. The yeast sterol pathway with the branch point for b-amyrin synthesis. Multiple steps are indicated by dashed lines. 0 2 4 6 8 10 12 0 50 100 150 200 250 Time (h) Squalene (µg·mL –1 culture) βamy1 βamy2 βamy3 βamy4 0 5 10 15 20 25 30 0 50 100 150 200 250 Time (h) Ergosterol (µg·mL –1 culture) 0 1 2 3 4 5 6 7 0 50 100 150 200 250 Time (h) β-amyrin (µg·mL –1 culture) 0 0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 0 50 100 150 200 250 Time (h) Attenuance at 600 nm AB CD Fig. 4. Determination of (A) squalene, (B) ergosterol, (C) b-amyrin, and (D) D 600 for strains bamy1 (BY4742, pESC-AaBAS), bamy2 (BY4742, pESC-AaBAS,P GAL1 -tHMG1), b amy3 (BY4742, pESC-AaBAS,P MET3 -ERG7), and bamy4 (BY4742, pESC-AaBAS,P GAL1 -tHMG1,P MET3 -ERG7 ). Error bars represent the SD for three independent cultures per strain. Engineering triterpene production in yeast J. Kirby et al. 1856 FEBS Journal 275 (2008) 1852–1859 ª 2008 The Authors Journal compilation ª 2008 FEBS should be possible to circumvent it by overexpressing ERG1 under the control of an independent promoter. Veen et al. [25] overexpressed ERG1 and tHMG1 together and observed a 50% increase in sterol concen- trations. We have subsquently tested this hypothesis by trans- forming the strain bamy4 with a high-copy expression vector harboring ERG1 under control of the GAL1 promoter (to create strain bamy5). A comparison between these two strains showed that b-amyrin pro- duction levels were essentially unchanged whereas squalene levels actually increased slightly in strain bamy5 (data not shown). This would indicate that flux from squalene to b-amyrin is not limited by ERG1 transcription levels, but the possibility remains that there is regulation of ERG1 at the protein level. It also is likely that there are other factors contributing to the lack of flux from squalene to b-amyrin. In particular, the availability of squalene for conversion by ERG1 may be limited by its biochemical state. In cases where tHMG1 has been overexpressed in yeast, squalene accumulates predominantly in an insoluble form that is not immediately available to the sterol pathway [25,27]. The sterol-acyl transferases ARE1 and ARE2 are responsible for esterification of excess squalene for storage in insoluble lipid particles [27]. Thus, it appears that attenuation of this process would be the next logi- cal step for further engineering triterpene production in S. cerevisiae. Additional studies into the possible regulation of ERG1 by 2,3-oxidosqualene at either post-translational or enzyme kinetic levels may also be warranted. Experimental procedures Isolation of a triterpene synthase gene from A. annua Leaf tissue from A. annua was collected predominantly from new growth at branch tips and immediately frozen in liquid nitrogen. The tissue was ground to a fine powder using a cooled mortar and pestle, and RNA was purified by the Qiagen Plant RNeasy extraction method using RLC buffer as supplied (Qiagen, Valencia, CA, USA). RNA was quantified and checked for integrity using the Bioanalyzer 2100 (Agilent, Foster City, CA, USA). A single triterpene- specific primer (TriF1; Table 1) was designed from an align- ment of various plant triterpene synthase protein sequences. For 3¢ RACE, two primers were used in conjunction with TriF1: a poly(dT) primer with a 5 ¢ ‘anchor’ sequence that has a melting temperature matching that of TriF1; and a primer composed of only the anchor sequence (Table 1). cDNA was synthesized from 3 lg of leaf total RNA using the polyT-anchor primer and Superscript II (Invitrogen, Carlsbad, CA, USA) with reverse transcription at 50 °C, followed by RNase H treatment. Touchdown PCR was car- ried out on the cDNA using the TriF1 and anchor primers by dropping the annealing temperature by 0.4 °C per cycle from 61 °Cto56°C for the first eight cycles, followed by 30 cycles of amplification with an annealing temperature of 56 °C. A product within the expected size range (1.4 kb, based on the average length of a triterpene synthase gene and a plant 3¢ UTR) was cloned into the TOPO TA vector (Invitrogen) and sequenced to reveal an ORF that appeared to encode a triterpene-synthase-like protein. The 5¢ end of the cDNA was recovered using the GeneRacer kit (Invitro- gen) according to the manufacturer’s guidelines, with the gene-specific primer TriRaceR1. The complete coding sequence of the candidate triterpene synthase gene was con- firmed by cloning and sequencing three PCR products, amplified using PFU Turbo (Stratagene, La Jolla, CA, USA) from three independent cDNA preparations. The sequence has been submitted to Genbank and is available under accession number EU330197. Expression of the candidate triterpene-synthase gene in S. cerevisiae The full-length coding sequence of the candidate triterpene synthase gene was amplified using the primers TricdsF and TricdsR, which contain the 5¢ restriction sites Eco RI and SacI, respectively. The sequence AACA was included immediately 5¢ to the start codon in TricdsF to provide a favorable translation start context [28]. The PCR product was cloned into the EcoRI and SacI sites of the yeast expression vector pESC-URA (Stratagene) under control of the GAL10 promoter and ADH1 terminator to create pESC-AaBAS. Following sequence verification, the plasmid was transformed into S. cerevisiae BY4742 by the lithium acetate method [29], and transformants were selected on synthetic complete minus uracil agar (SC-URA). Synthetic complete media were made by adding 6.7 g Æ L )1 Difco yeast nitrogen base (Becton, Dickinson & Co., Sparks, MI, USA) to a complete supplemental mixture (MB Biomedicals, Solon, OH, USA) of vitamins, minerals and amino acids, with the appropriate amino acid dropped out. Transformed yeast strains were maintained on SC medium with 2% d-glucose as carbon source, and induced by inoculating into SC + 2% d-galactose at D 600 of 0.02–0.03. Modification of the sterol biosynthesis pathway in S. cerevisiae A strain containing a soluble, truncated form of HMG- CoA reductase 1 (HMG1) under control of the GAL1 pro- moter was constructed by transformation of BY4742 with the integrating plasmid pd-tHMGR and selection for loss J. Kirby et al. Engineering triterpene production in yeast FEBS Journal 275 (2008) 1852–1859 ª 2008 The Authors Journal compilation ª 2008 FEBS 1857 of the URA3 selection marker using 5-fluoroorotic acid, as described previously [17]. The native promoter for the S. cerevisiae lanosterol syn- thase gene, ERG7, was replaced with the methionine-sup- pressible promoter P MET3 to create the strain P MET3 -ERG7. The strategy used essentially follows that described by Gardner and Hampton [21]. Genomic DNA from strain BY4742 served as a template for amplification of the first 422 bp of the ERG7 cds using the primers ERG7F and ERG7R (Table 1). The amplified fragment was cloned into the NcoI and ClaI restriction sites of the vector pRS-ERG9 [17], thus replacing the ERG9 cds fragment with the ERG7 cds fragment, 3¢ to the MET3 promoter. For integration into S. cerevisiae, the vector was digested at the unique BbvCI site in the ERG7 cds to facilitate homologous recom- bination with the native ERG7 gene. Upon transformation into S. cerevisiae, the successful promoter replacement was confirmed by PCR using a P MET3 forward primer and an ERG7 reverse primer. Extraction, identification, and quantitation of b-amyrin and sterols A single method was developed to extract and quantify b-amyrin and the native yeast sterols squalene and ergos- terol. Yeast culture (1 mL) in a microfuge tube was centri- fuged for 1 min at 17 900 g to pellet cells. The cells were resuspended in 0.6 mL of a fresh solution of 20% (w ⁄ v) KOH in 50% ethanol containing 30 lgÆmL )1 cholesterol as an internal standard. The cells were boiled for 5 min in this solution in 2 mL screw-cap tubes. After cooling, the sterols and b-amyrin were extracted by vortexing with 0.6 mL dodecane (Sigma, St Louis, MO, USA) for 5 min at room temperature. The dodecane phase was transferred to a glass vial and directly subjected to GC ⁄ MS (GC model 6890, MS model 5973 inert, Agilent). An aliquot of the sample (1 lL) was injected into a DB5-MS column (Agilent) operating at a helium flow rate of 1 mLÆmin )1 . The oven temperature was held at 80 °C for 1 min after injection, and was then ramped to 280 °Cat20°CÆmin )1 , held at 280 °C for 20 min, ramped to 300 °Cat20°CÆmin )1 and finally held at 300 °C for 2 min. The MS ion source was held at 300 °C throughout, with the quadrupole at 200 °C and the GC ⁄ MS transfer line at 280 °C. Full mass spectra were generated for metabolite identification by scanning within the m ⁄ z range of 40–440. For quantification of metabolites, samples were run in selected ion mode, detecting ions 203, 218, and 426. Standard curves for b-amyrin, squalene and ergosterol were run at the start and end of each batch of samples. References 1 Phillips DR, Rasbery JM, Bartel B & Matsuda SP (2006) Biosynthetic diversity in plant triterpene cycliza- tion. Curr Opin Plant Biol 9, 305–314. 2 Fujioka S & Yokota T (2003) Biosynthesis and meta- bolism of brassinosteroids. 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Mol Microbiol 19, 1225– 1239. 29 Gietz RD & Schiestl RH (2007) Quick and easy yeast transformation using the LiAc ⁄ SS carrier DNA ⁄ PEG method. Nat Protoc 2, 35–37. J. Kirby et al. Engineering triterpene production in yeast FEBS Journal 275 (2008) 1852–1859 ª 2008 The Authors Journal compilation ª 2008 FEBS 1859 . Engineering triterpene production in Saccharomyces cerevisiae – b-amyrin synthase from Artemisia annua James Kirby 1 , Dante W. Romanini 2 , Eric M. Paradise 1,3 and Jay D. Keasling 1,3,4,5 1. synthase gene from A. annua and its expression in S. cerevisiae. Our findings on engineering overproduction of b-amyrin in S. cerevisiae should be relevant to the production of any triterpene. Results Isolation. confirmed the encoded enzyme as a b-amyrin synthase. Through engineering the sterol pathway in S. cerevisiae, we explore strategies for increasing triterpene production, using AaBAS as a test case. By