... and HinfI for 3 h and recovered byethanol precipitation. The precipitated DNA was dissolvedin 3 lL of water and added into the ligation mixture[100 ng each of landmark- and HinfI-adapter and ... reaction volume containing 5 lLof10· PCR buffer (Takara, Tokyo, Japan), 4 lL of 2.5 mmeach dNTP mixture, 10 pmol each of CasA- and CasB-specific primers, 1 lL of ligation solution, and 1.25 U of Taq ... duringestablishment of symbiosisHiroyuki Ichida1,2, Tomoki Matsuyama3, Tomoko Abe2 and Takato Koba11 Graduate School of Science and Technology, Chiba University, Matsudo, Japan2 Accelerator Application...