... dynamics with single monomer resolution. Cell126, 515–527.30 Qiao W, Mooney M, Bird AJ, Winge DR & Eide DJ(2006) Zinc binding to a regulatory zinc-sensing domainmonitored in vivo by using FRET. ... proteins, inclu-ding COL-19 (Fig. 10G), nonerythrocyte spectrin(Fig. 10E), filamin A (Fig. 10C), and a -actinin(Fig. 10A), and expression systems including HEK-293,3T3 and C. elegans, and the insertion ... wassubcloned into the prokaryotic expression vectorPinPoint Xa-3 (Promega, Madison, WI, USA), using BamHI and NotI restriction sites, which were introducedinto FRET DNA fragment by using the following...