... 95%
neutrophil granulocytes.
Measurements of reactive oxygen species
Superoxide generating activity was spectrophotometrically
assayed by monitoring SOD-inhibitable ferricytochrome c
reduction ... kinase activity of PAK were stimulated by
binding to activated Rac/Cdc42, which thereby directly
modulates the enzyme activity [7–11]. However, Rho did
not show binding activity to PAK [8]. In ......
... binding
kinetics.
Keywords: high -pressure stopped-flow; cytochrome P450;
CO ligand binding; protein dynamics.
Cytochromes P450 represent a big superfamily of heme-type
monooxygenases that catalyze the conversion ... group and the hydroxyl group of the
amino-acid residue Tyr-96, and (b) by hydrophobic contacts
of its methyl groups C-8, C-9 to Val295 and Asp297 in the
b
3
sheet, and of the met...
... Claude Balny
3
1
Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kyushu University, Fukuoka, Japan;
2
Department of Applied Biological Chemistry, Faculty of Agriculture, ... ATP, respectively. Ch anges
in the intensity of ¯uorescence of F-actin whilst under
pressure suggested that eADP±F-actin was initially depoly-
merize d to eADP±G-actin; subsequently there was...
... N-(p-(2-benzimidazolyl)phenyl)
maleimide; C
12
E
8
, octa-ethyleneglycol mono n-dodecyl ether; H±T
exchange, hydrogen±tritium exchange; pNPP, p-nitrophenylphos-
phate; soy-PtdCho, soybean phosphatidylcholine.
(Received ... circles).
The increase in energy transfer induced by a pressure of
100 M Pa or higher (Fig. 4) may be explained simply by a
decrease in the distance between Cys964 and Ly...