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Báo cáo y học: " Polysaccharides from the root of Angelica sinensis protect bone marrow and gastrointestinal tissues against the cytotoxicity of cyclophosphamide in mice

Báo cáo y học:

Báo cáo y học: " Polysaccharides from the root of Angelica sinensis protect bone marrow and gastrointestinal tissues against the cytotoxicity of cyclophosphamide in mice

... reserved Research paper Polysaccharides from the root of Angelica sinensis protect bone marrow and gastrointestinal tissues against the cytotoxicity of cyclophosphamide in mice Marco K. C. Hui, ... marrow and the gastrointestinal tissues from the cytotoxicity of CY in mice. We also profiled the changes of the expression of growth factors in gastric tissues in response to the damage by CY and ... the above findings not only provide a fundamental insight into the mechanism of CY-induced systemic cytotoxicity, particularly in the gastrointestinal system, but also propose a role for polysaccharides...
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Báo cáo Y học: ORF6 from the clavulanic acid gene cluster of Streptomyces clavuligerus has ornithine acetyltransferase activity potx

Báo cáo Y học: ORF6 from the clavulanic acid gene cluster of Streptomyces clavuligerus has ornithine acetyltransferase activity potx

... assay,indicating the equilibrium position for the N-acetylorni-thine:ornithine mixture was approximately 2 : 1 as deter-mined by integration of the hydrogens of the a-acetyl CH3group of the N-acetylated ... (2S,3R)-3-hydroxy-N-acetylarginine is shownboxed.Scheme 2. The role of OAT (ARGJ) in the biosynthesis of arginine.ORF6 carries out the same reaction. The proposed acyl-enzyme in- termediate ... procedure of Chinard [24] which employs ninhydrin todetermine the presence of ornithine. Following incubation,derivatization was accomplished by the addition of 0.4 mL MilliQ water and 0.5 mL ninhydrin...
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Báo cáo Y học: Ferritin from the spleen of the Antarctic teleost Trematomus bernacchii is an M-type homopolymer doc

Báo cáo Y học: Ferritin from the spleen of the Antarctic teleost Trematomus bernacchii is an M-type homopolymer doc

... erritins. Thus,H c hains predominate in spleen and liver at variance withthepresenceofL chainsinthesameorgansofmammals[6].Interestingly, a study of cold-inducible gene expression of rainbow ... protein indicated for the N-terminalpeptide MDSQVR a value of m/z 777, which points t o the presence of acetylme thionine. This r esidue is commonlyfound in the N-terminus of eukaryotic proteins ... bridgeformed within the four-helix bundle of the L c hains betweenK62 and E107 and thought to play a special role in conferring thermal stability [20]; in the T. bernacchiiM c hain, just as in the human...
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Báo cáo Y học: Hemocyanin from the keyhole limpet Megathura crenulata (KLH) carries a novel type of N-glycans with Gal(b1–6)Man-motifs doc

Báo cáo Y học: Hemocyanin from the keyhole limpet Megathura crenulata (KLH) carries a novel type of N-glycans with Gal(b1–6)Man-motifs doc

... Man shown by methylation analysis. Consid-ering the linkage position of b-Gal residues found in the other compounds and the common route of biosynthesis of N-linked glycans, it may be postulated ... N-glycans.Hemocyanins are oxygen-transporting proteins found in many arthropod and mollusc species [1]. Binding of oxygen ismediated by binuclear copper-binding sites, resulting in the characteristic ... glyco-protein-N-glycan cores. The precise substrate specificity of these enzymes remains to be investigated.Gal(b1–6)Man-motifs have neither been detected in the hemocyanin-linked glycans of the pulmonate...
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Báo cáo y học:

Báo cáo y học: " WT1 PEPTIDE VACCINATION IN COMBINATION WITH IMATINIB THERAPY FOR A PATIENT WITH CML IN THE CHRONIC PHASE"

... peptides. An in vitro study as to the cytotoxicity of lymphocytes induced by mixed lymphocyte peptide culture demonstrated that cultured lymphocytes possessed cytotoxicity against WT1 expressing leukemia ... Percent cytotoxicity of the assay was calculated by the following formula. : % cytotoxicity = [(absolute number of viable target cells in the tube containing target cells only –absolute number of ... flow cytometry (BD Bios-ciences) and 10,000 events were collected, the data of which was analyzed by FACSDiva software (BD Bi-osciences). Cytotoxicity assay To estimate anti-WT1 cytotoxicity of...
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Báo cáo khoa học: Peptides from purified soybean b-conglycinin inhibit fatty acid synthase by interaction with the thioesterase catalytic domain pot

Báo cáo khoa học: Peptides from purified soybean b-conglycinin inhibit fatty acid synthase by interaction with the thioesterase catalytic domain pot

... near physiologicconditions, meaning that they bind to the unoccupiedenzyme and reduce substrate turnover. The TE domaincoordinates the terminal step of fatty acid synthesis byhydrolyzing palmitate ... accommodaterelatively large inhibitors and that the TE domain isa better target for these types of peptide inhibitors.To confirm the binding of these peptide inhibitors in the TE domain, a biochemical enzyme inhibition ... werecustom synthesized and their FAS inhibitory activitiestested. Figure 1 shows the FAS inhibitory activity of the three synthetic peptides and the C75 positive con-trol. The FAS inhibitory responses...
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Báo cáo Y học: A novel DNA repair enzyme containing RNA recognition, G-patch and specific splicing factor 45-like motifs in the protozoan parasite Toxoplasma gondii potx

Báo cáo Y học: A novel DNA repair enzyme containing RNA recognition, G-patch and specific splicing factor 45-like motifs in the protozoan parasite Toxoplasma gondii potx

... tachyzoites. The tachyzoites differentiate intoencysted bradyzoites in response to the immune systemattack during disease progression. They will remain in the brain and other organs during the lifetime ... of infected hosts. The reactivation of encysted bradyzoites into activelyreplicating and cytolytic tachyzoites can trigger a diseasewith severe clinical syndromes leading in many cases to the death ... used for the amplification of the ORFs of TgDRE and the housekeeping gene a-tubulin using serial dilutions of cDNAsprepared from tachyzoites and bradyzoites isolated from brain of infected mice. Ó...
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Báo cáo Y học: GNA33 from Neisseria meningitidis serogroup B encodes a membrane-bound lytic transglycosylase (MltA) pdf

Báo cáo Y học: GNA33 from Neisseria meningitidis serogroup B encodes a membrane-bound lytic transglycosylase (MltA) pdf

... cleave the polysaccharidebackbone of the peptidoglycan layer, lytic transglycosylasesare thought to play a role in synthesis and degradation of the murein sacculus. It has been proposed that lytictransglycosylases ... lysozyme where the glycosyl moiety is transferred to H2O, cleavage by lytictransglycosylases is followed by an intramolecular transgly-cosylation [10]. In this reaction, the glycosidic linkagebetween ... mM and 1.0M in buffer I were applied in 5 · 1.0 mL aliquots, and the eluates retained for analysisby SDS/PAGE, penicillin-binding assay, and Western blot.Penicillin-binding assayPenicillin-binding...
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Báo cáo y học:

Báo cáo y học: "Strict glycaemic control in patients hospitalised in a mixed medical and surgical intensive care unit: a randomised clinical trial"

... mortality at 28 days was 32.4% (81 of 250) in thestandard insulin group and 36.6% (93 of 254) in the intensiveinsulin group. ICU mortality was similar for patients of thestandard insulin group and in ... reduction in the rate of ICU-acquired infections:33.2% in the standard insulin therapy group compared with27.17% in the intensive insulin therapy group (RR: 0.82;95%CI: 0.63 to 1.07). The rate of hypoglycaemia ... unaware of the randomisedschedule and the block size.InterventionsPatients were randomly assigned to receive either standardinsulin therapy or intensive insulin therapy. Both groupsreceived insulin...
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Báo cáo y học:

Báo cáo y học: "Primary lower limb lymphedema: a focus on its functional, social and emotional impac"

... found interesting to review known cases of lymphe-dema in the island of Crete. These reports f ocu s on two cases of primary lower limb lymphedema, by discussing the overall physical and mental ... lymphedema, the same as in other chronic illnesses and conditions. Assessing not only the severity of the physical limitation but also the re-lated psychosocial dimensions and quantifying the ... passed from the mo-ment of the diagnosis may have offered the first pa-tient the chance to cope better with the psychological aspects of his condition over time. In the past, the burden of the...
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