... cleave the polysaccharidebackbone of the peptidoglycan layer, lytic transglycosylasesare thought to play a role in synthesis and degradation of the murein sacculus. It has been proposed that lytictransglycosylases ... lysozyme where the glycosyl moiety is transferred to H2O, cleavage by lytictransglycosylases is followed by an intramolecular transgly-cosylation [10]. In this reaction, the glycosidic linkagebetween ... mM and 1.0M in buffer I were applied in 5 · 1.0 mL aliquots, and the eluates retained for analysisby SDS/PAGE, penicillin-binding assay, and Western blot.Penicillin-binding assayPenicillin-binding...