... oligonucleotides were designed based on the sequenceof the A. aculeatus b- 1,4-endogalactanase encoding gene(5¢-CTCTTCTCTCTTGCTCTTG-3¢ and 5¢-GTTCGTCTCCACAACCAC-3¢, respectively) and used in PCRs ... (Darmstadt,Germany).D-Glucuronic and D-galacturonic acid werefrom Fluka (Buchs, Switzerland). Mellibiose, raffinose,stachyose,L-arabinose, gum arabic, gum karaya, locustbean gum, and beechwood xylan ... with b- 1,6-linkedD-galactopyranose residues [1]. Both types canbe substituted with a)1-,3-linkedL-arabinofuranose chains.Type I arabinogalactan is degraded by b- 1,4-endogalacta-nase and b- galactosidase. b- 1,4-Endogalactanases...