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Microbial Production of Amino Acids in Japan

Microbial Production of Amino Acids in Japan

Microbial Production of Amino Acids in Japan

... acetic acid in the cells of the Microbial Production of Amino Acids in Japan 73 Table 1. Amino acid production in Japan and the world in 1996 Amino acid Amount (tons/year) Method Japan World ... strain of E. coli which lacks l-proline-degrading enzyme was obtained and used for the host cells of produc- tion of l-hydroxyproline. Microbial Production of Amino Acids in Japan 83 Using E. ... gene-, protein-, and metabolic engineer- ing or by combinations of these types of engineering (hydroxy-l-proline) In this paper some representative examples of microbial production of amino acid...
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Báo cáo khóa học: Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation ppt

Báo cáo khóa học: Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation ppt

... the combination of amino acids at positions3 and 6 might be a critical determinant for protein N-myristoylation. In the present study, to examine the effect of thecombination of amino acids ... TNFmutant in which 9 amino acids following the initiating Metwere changed to Ala, was used as the starting sequence toevaluate the roles of the amino acids at distinct positions in protein N-myristoylation ... metabolic labeling of model substrate proteins in an in vitro translation system. In this case, to avoid theeffects of amino acids at other positions in the N-terminalregion on protein N-myristoylation,...
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Báo cáo khoa học: Identification of amino acids in antiplasmin involved in its noncovalent ‘lysine-binding-site’-dependent interaction with plasmin pptx

Báo cáo khoa học: Identification of amino acids in antiplasmin involved in its noncovalent ‘lysine-binding-site’-dependent interaction with plasmin pptx

... before 50% of theplasmin activity was inhibited).Computer model of the C-terminal 40 amino acids in antiplasminA computer model of the C-terminal 40 amino acids in antiplasmin [11] was constructed ... lysine-binding-site-dependentinteraction between plasmin and antiplasmin in detailby constructing seven single-site mutants of antiplasmin.Fig. 3. Computer model of the C-terminal 40 amino acids ... lysine-binding-site-mediated interaction betweenplasmin and antiplasmin.Molecular modelling of the C-terminal portion of antiplasminThe amino- acid sequence of the C-terminal 40 residues in antiplasmin...
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Báo cáo Y học: Amino acids 3–13 and amino acids in and flanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf

Báo cáo Y học: Amino acids 3–13 and amino acids in and flanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf

... (2001)Disrupted amino- and carboxyl–terminal interactions of theandrogen receptor are linked to androgen insensitivity. Mol.Endocrinol. 15, 923–935. Amino acids 3–13 and amino acids in and flanking the23FxxLF27motif ... Alanine scanning of AR17–32: amino acids flanking F23, L26 and F27 modulate AR N/C interaction. (A) GalAD-ARpeptide fusion proteins in the yeast protein interaction system. (B) Interaction of GalAD-ARpeptides ... and 25 increased the interaction capacity. Allalanine substitutions of amino acids flanking23FQNLF27reduced the binding to AR LBD. Most prominent inhi-bitory effects were found for amino acid...
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Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf

Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf

... pattern. Amino acids withsimilar characteristics were grouped together as follows:aromatic amino acids (Phe, Tyr, and Trp); negativelycharged amino acids (Asp and Glu); positively charged amino acids ... pro-vided insights into important enzyme–substrate interac-tions. For example, molecular modeling of HCinteracting with angiotensin (Ang) I has led to conclu-sions regarding the S2¢ binding site of ... by an amino acid with an uncharged side chain, a preference formainly aliphatic amino acids was observed. However,a weak preference for acidic P2¢ amino acids stillremained for the two single...
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Báo cáo khoa học: D-Amino acids in the brain: the biochemistry of brain serine racemase potx

Báo cáo khoa học: D-Amino acids in the brain: the biochemistry of brain serine racemase potx

... calcium binding areshown in orange and those involved in nucleotide binding are shown in red. Residues involved in the binding to the PLP moiety are shown in blue, whereas those involved in protein–protein ... domain-containing protein was found to interact with serineracemase, also requiring the C-terminal binding motif[30]. Protein interacting with C kinase 1 (PICK1) con-tains one PDZ domain that ... PDZ interactionswith the C-terminal amino acid triplet of serine werecrucial for binding, but instead, the interaction wasestablished with its N-terminal 66 residues. Binding of Golga3 increases...
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Báo cáo khóa học: O-acetylation and de-O-acetylation of sialic acids in human colorectal carcinoma docx

Báo cáo khóa học: O-acetylation and de-O-acetylation of sialic acids in human colorectal carcinoma docx

... incubating 50 lg of protein in 30 lLof50mMpotassium phosphatebuffer, pH 7.0, containing 50 mMKCl, protease andesterase inhibitors, and [3H]AcCoA (0.2 lCi, 8.3 lM)at37 °C for 15 min. ... activities of OAT:OAE-L provides a significant insight into the regula-tion of this important modification in normal as well as in diseased tissue. Based on this information a reduction in orhalting of ... The resulting residue wasresuspended in ice-cold water and sialic acids purified aspreviously described [24]. The incorporation of [3H]acetateinto the glycerol side chain of sialic acids was...
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Báo cáo khoa học:

Báo cáo khoa học: "ASPECTS AN OF CLAUSE POLITENESS IN JAPANESE: TREATMENT EXTENDED IN QUIRY SEMANTICS" potx

... corresponding minimal points of grammatical alternation. The levels of coding are distinct and incorporate distinct aspects of the mean- ing and construction of the linguistic units involved. ... currently in Lisp, of an actual interrogatlon of a knowledge base. Typically, constructing an inquiry proceeds first by means of suc- cees|ve approximations in informal terms, glossed in English, ... domain: The ex- pression of politeness in Japanese clauses As part of a proposed text generation project in Japanese at Kyoto University, some fragments of a systemlc-functlonal grammar of Japanese...
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The Production of Child Health in Kenya: A Structural Model of Birth Weight potx

The Production of Child Health in Kenya: A Structural Model of Birth Weight potx

... sizes of effects of commodity prices on health depend on (a) magnitudes of changes in demand for health inputs following price changes and on (b) sizes of the marginal products of health inputs. ... easily understood by noting that when both m1 and Q are increasing, birth weight is increasing at the rate, θ. As long as m1 is increasing, every unit increase in Q increases birth weight ... holding and with rent income but declines with cattle wealth. Land and cattle assets increase the marginal product of woman’s time, thereby raising the opportunity cost of seeking vaccination...
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