... ligands. These are one watermolecule and five amino acids [12]. The iron ligands are the carboxyl oxygen of the C-terminal isoleucine (orvaline) residue, the nitrogen atoms of two histidineresidues ... was prepared by grinding of mycelia in liquidnitrogen, extracting with the RNeasy plant kit, and checking for integrity by agarose gel electrophoresis. About2.5 lg of total RNA and 1 U enhanced ... two bands of 67 and 73 kDa. Mn-LOX-Gga yielded mainly the 67 kDa pro-tein, whereas Mn-LOX-Ggt yielded both with equal inten-sity, possibly due to incomplete deglycosylation [34]. The total...