... generation of the pQE-32-Mydj2 plasmid. The accuracy
of the resu lting construct ( pQE-32-Mydj2) was v erified by
DNA sequencing, and the plasmid was subsequently used to
overexpress M ydj2–6·His in Escherichia ... f binding and release regulated b y their
DnaJ cochaperones [2,3]. Based on the existense of three
dinstict domains, namely: the highly conserved J domain...
... min) BotXIV induced a plateau potential lasting for
110 ms followed by a long lasting and a very slowly decayed
depolarization. In the case of the BotXIVM8-10, the evoked
ÔplateauÕ is only 3–6 ms ... was decreased both during the
activation and inactivation mechanisms, in presence of
BotXIV the sodium current voltage dependence was
decreased only in the case of...
... of a phycobiliprotein lyase: both the attachment of phy-
cocyanobilin and the isomerization to phycoviolobilin are cata-
lyzed by the proteins PecE and PecF encoded by the
phycoerythrocyanin operon. ... photochemistry; energy transfer.
Phycobiliproteins are a class of chromoproteins bearing
covalently bound linear tetrapyrrole (phycobilins) chro-
mophores. They are predomina...
... to wild-type
(Table 1). Moreover, when the inhibitors could in principle
overcome the barrier of the channel constriction and act
directly in the active site cavity, as in the case of the C435S
mutant, ... C435
may occur.
The series of thiol-reacting agents used to study access of
the substrate to the active site of SHC was also found to be a
useful t...
... moiety of LPS in the induction of
cytokines, including tumor necrosis factor, and the phos-
phate residue at the 4¢-position is a critical site for the
activity [24]. Therefore, the monophosphoryl ... monitored by measuring phosphorus and hexose
contents. The eluates were combined, dialyzed and lyo-
philized. The combined fraction was used as an OPS-rich
fraction...