... Ne1 of tryptophans 47, 52, 131, 20 4 are 33.6 A˚, 22 .7 A˚, 25 .1 A˚, 29 .5 A˚,respectively. In the dimeric form, the distances of trypto-phan residues 47, 52, 131, 20 4 from the flavin cofactor ... Palfey, B.A. & Carey, P.R. (1999) UsingRaman spectroscopy to monitor the solvent-exposed and ÔburiedÕforms of flavin in p-hydroxybenzoate hydroxylase. Biochemistry38, 16 727 –167 32. Ó FEBS 20 03 ... catalytic efficiency of the thermophilic enzymes.Another way would be to identify the rate-limiting step in enzyme catalysis. This information may indicate asuitable amino-acid residue in the...