... investigatedextensively. In gymnosperms, such as grand fir, terpenesare produced in resin ducts [2,3]. Their biosynthesis isinduced upon wounding [4–6], indicating their role in thedefence against bark beetle infestation. ... afterdrying dissolved in 5 mMTris, pH 6.8, 0.2% (w/v) SDS and1% glycerol. Protein concentration was determined usingthe BCA Protein assay kit using BSA as protein standardreference, according ... hexane. To investigatethe linearity of the assays with time the enzymes wereincubated for 0, 10, 20, 30, 45 and 60 min at 30 °C. Fortesting the pH optimum of the enzymes they wereincubated in Mopso...