... [7,8].Ribotoxins of the a-sarcin family display a high degree of sequence similarity although they are secreted by a variety of different moulds [9–11], i.e. restrictocin and mitogillin,two other members of ... donor-to-acceptor,[N-(7-nitro-2-1,3-benzoxadiazol-4-yl)-dimyristoylglycero-phosphoethanolamine (Nbd-Myr2GroP Etn) and N-(lissaminerhodamine B sulfonyl)-diacylglycerophosphoethanolamine(rhodamine-GroP Etn), respectively, fluorescence energytransfer ... thewild-type protein [30,34]. Fungal wild-type a-sarcin wasproduced and purified as previously reported [30,35,36].Polyacrylamide electrophoresis, tryptic digestions, acidhydrolysis of proteins,...