Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"
Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"
... con-centration. In vitro releasing study The release of pravastatin as well as hemoglobin from carrier erythrocytes were determined as follow-ing, 1 ml of packed drug-loaded erythrocytes was diluted ... temperature as well as concentration on the loading of pravastatin in human erythrocytes to be using them as systemic sustained release delivery system for this drug. After the loading process is performed ... suggesting that erythrocytes can be used as prolonged release for pravastatin. Key words: drug delivery, erythrocytes, pravastatin, osmotic fragility Introduction The statin drugs are used in...
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Báo cáo Y học: Characterization of a low redox potential laccase from the basidiomycete C30 pptx
... responsible for thefour electron reduction of dioxygen [3]. These enzymes arecommon in plants, fungi, insects and bacteria [1]. In plants,they may mainly play a role in lignification [4] whereas in fungi ... differences in kinetics observed may bethe consequence of the variability of certain of the aminoacids involved in the substrates channel in specific enzymes.A substantial variation in the folding of ... [15]. The fungus was cultivated at30 °C on a reciprocal shaker (50 r.p.m.) for 5 days [15].Laccase activityThe routine assay for laccase was based on syringaldazineoxidation in 0.1Mphosphate...
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Báo cáo Y học: Characterization of selenoprotein P as a selenium supply protein docx
... recombinant human insulin and human transferrin from Wako, Osaka, Japan; and nickel-nitrilotriacetic acid agarose from Qiagen Inc., Chatsworth,CA, USA. Recombinant human thioredoxin was kindlyCorrespondence ... requiring iron [41]. Albumin is believed tosupply many low-molecular-mass substances such as metals,amino acids and fatty acids [42]. Transferrin and albuminnoncovalently bind iron and many low-molecular-masssubstances, ... of total Se in human serum (or plasma)accounted for by SeP. Using heparin–agarose, SeP accoun-ted for 40% of the Se applied to the column [37]. The SeP in the plasma of Chinese men of varying...
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Báo cáo Y học: Characterization of four substrates emphasizes kinetic similarity between insect and human C-domain angiotensin-converting enzyme pptx
... conserved as insect ACE can hydrolyse mammalianACE substrates such as angiotensin I, substance P, lutein-izing hormone releasing hormone, enkephalins and enkeph-alinamides, hereby displaying the ... as present in the N-terminal part of several y yolk proteins. An antiserum raised against Neb-ODAIF-11)9immunostainedone out of three yolk protein bands of SDS/PAGE-separ-ated y haemolymph ... PAP, peroxidaseantiperoxidase; PVDF, polyvinylidene difluoride; sACE, somaticangiotensin converting enzyme; tACE, testicular angiotensinconverting enzyme; TMOF, trypsin modulating oostatic...
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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
... protein. Most of the protein was expressed in inclusion body. The fusion protein in inclusion body was purified by using High-Affinity Nickel Iminodiacetic Acid Resin and refolded in urea gradient ... University Institute of Life Sciences (Hangzhou, China). Mouse anti-His-tag monoclonal antibody was purchased from Novagen Company (Germany). Streptozocin was obtained from Sigma Company ... supernatant and inclusion body. Part of the production was applied to 12% SDS–PAGE analysis and Western blot analysis. (A) SDS-PAGE analysis: Most of the fusion protein was found in inclusion...
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Báo cáo y học: "Characterization of erythrovirus B19 genomes isolated in liver tissues from patients with fulminant hepatitis and biliary atresia who underwent liver transplantation"
... Hepatitis. Clinical epidemiology of ful-minant hepatitis in Japan before the substantial introduction of liver transplantation: an analysis of 1309 cases in a 15-year na-tional survey. Hepatol ... These findings indicate the difficulty in amplifying the sequences in these re-gions. In our study, we attempted to amplify each ex-tremity, but failed. Interestingly, phylogenetic analysis based ... B19 -AN4 1, AB126268 for B19 -AN5 6, AB126269 for B19 -AN6 6, AB126270 for B19 -AN8 5 and AB126271 for B19 -AN8 7. 4. Discussion The etiology of hepatitis remains obscure in 3-10% of cases in Europe [5] and...
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Báo cáo y học: "Characterization of N200 and P300: Selected Studies of the Event-Related Potential Salil H. Patel 1 and Pierre N. Azzam 2"
... proliferation of high-density EEG methods, and interdisciplinary interest in its application as a prognostic, diagnostic, and investigative tool, an understanding of the underpinnings of P300 and N200 physiology ... utilizing the auditory oddball paradigm. In one such study examining frequency and intensity deviation, changes in inter-stimulus interval (ISI) and intensity in auditory oddball detection tasks ... little, if any, effect on the latency and amplitude of the evoked MMN, the elicited N2b was found to continuously increase in latency and decrease in amplitude with increasing age. These findings suggest...
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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
... noinformation is available on the regulation of DNase I gene (DNASE1)expression in vivo or in vitro. We first mapped the transcription start sites of DNASE1 in human pancreas and in the DNase ... orhypoxic conditions prior to the measurement of the activit-ies of fire y and Renilla luciferase. Cell lysis and luciferaseassays were performed using the Dual Luciferase ReporterAssay System ... dele-tion of 39 bp. The thick lines indicate intron 6 of 75 bp in fragmentsB and C, 3¢ portion of 35 bp in intron 5 in fragments C and D, and3¢ portion of 25 bp in intron 6 in fragments F and I....
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Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx
... milliliter of S-hexyl glutathione agarose. In all cases, protein purity wasdetermined by SDS/PAGE and protein concentration wasmeasured by bicinchoninic acid method following manu-facturers indications ... 8.0, and eluted by adding 10 lLofthesame buffer containing 5 mMGSH, GSSG or S-hexyl GSH.Ten microliters of the original protein, binding assaysupernatant and eluate were analyzed by SDS/PAGE.Expression ... expressedmore in parasitic stages than in free living stages during theS. mansoni life cycle.Characterization of recombinant SmGSTO enzymaticactivityRecombinant GSTO was used to assay its enzymaticactivity....
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Tài liệu Báo cáo Y học: Characterization of a cloned subtilisin-like serine proteinase from a psychrotrophic Vibrio species doc
... behaviour, indicating thatthe same disulfide(s) are involved in maintaining theintegrity of active protein structure in both wild-type andrecombinant VPR.The wild-type and recombinant forms of the ... in psychrophilic enzymes. Glycine residues are highly con-served in the sequences of the proteinases compared in this study. For example, of the 37 glycines present in theproteinase domain of ... additionally contains Cys277 and Cys281, andCys351 and Cys362 in the C-terminal domain, that suppo-sedly also form disulfide bonds as the enzyme has not beenfound to contain any free thiol groups...
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