Báo cáo y học: "A Comparison of Immuncapture Agglutination and ELISA Methods in Serological Diagnosis of Brucellosis"
Báo cáo y học: "A Comparison of Immuncapture Agglutination and ELISA Methods in Serological Diagnosis of Brucellosis"
... has been recently introduced and used in serological diagnosis. The aim of this study was to compare diagnostic values of ELISA Ig M and Ig G and im-muncapture agglutination tests with ... test by virtue of the fact that it determines blocking antibodies in the diagnosis and follow-up of brucellosis. Key words: Brucellosis, immuncapture, ELISA, IgG, IgM, serologic diagnosis INTRODUCTION ... The most widely used of these are Standard Tube Agglutination and Coombs anti-brucella tests. Whereas ELISA Ig M and Ig G tests have been in use for a long time, immuncapture agglutination test...
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Báo cáo Y học: A comparison of the urea-induced unfolding of apo¯avodoxin and ¯avodoxin from Desulfovibrio vulgaris potx
... Ireland1The kinetics and thermodynamics of the urea-inducedunfolding of ¯avodoxin and apo¯avodoxin from Desulfo-vibrio vulgaris were investigated by measuring changes in ¯avin and protein ... vulgaris showing the relative positions of the FMN, tryptophan side chains and tyrosineside chains. TheFMNisshowninyellow,tryptophan side chains in magenta, and tyrosine side chains in green. ... thecorresponding reactions of its apoprotein. This ¯avodoxincontains two residues of tryptophan and ®ve residues of tyrosine. A tyrosine side chain (Y9 8) is almost coplanar withthe face of the dimethylisoalloxazine...
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Báo cáo y học: " A Dietary Supplement Containing Standardized Phaseolus vulgaris Extract Influences Body Composition of Overweight Men and Women"
... dietary changes limiting caloric intake, increased physical activity, behavioral therapy, phar-macotherapy, and, in extreme cases, surgery [1]. Al-though the availability and popularity of natural ... our study (Phase 2TM) is a standardized white kidney bean extract prepared using heated processing conditions to substantially inactivate hemagglutinat-ing activity (HA) and trypsin inhibiting activity ... Theoretically, they may promote weight loss by interfering with the breakdown of complex carbohydrates thereby reducing, or at least slowing, the digestive availability of carbo-hydrate-derived...
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Báo cáo Y học: A critical motif for oligomerization and chaperone activity of bacterial a-heat shock proteins pot
... with aA-crystallin and Hsp27 [45] indicatethat the dimeric building block of many a-Hsp proteins isformed by interacting bstrands in the a-crystallin domain.Although this may also apply to the ... portion of the N-terminal region in vicinity of the a-crystallindomain.To ensure that efficient dimerization requires solelya-crystallin domain and N-terminal region and not theC-terminal extension, ... a-crystallin domain or the a-crystallin domainincluding either the N-terminal region or the C-terminalextension were constructed in a similar manner. Mutationsleading to single and double amino-acid...
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Báo cáo y học: " High blood pressure, antihypertensive medication and lung function in a general adult population"
... F4study was conducted between 2006 and 2008.The investigations were carried out in accordance withthe Declaration of Helsinki, including written informedconsent of all participants. All study methods ... the effects of high bloodpressure and antihypertensive medication. Thus, it allowsonly statements about a single point in time and does notallow evaluating the effect of long-standing high bloodpressure. ... Care, Pulmonary Medicine andInfectious Diseases, University of Greifswald, Greifswald, Germany.7CentralHospital of Augsburg, MONICA/KORA Myocardial Infarction Registry,Augsburg, Germany.8Ludwig-Maximilians-University,...
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Báo cáo khoa học: A steady-state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae ppt
... Venkatesh1,21Department of Chemical Engineering and 2School of Biosciences and Bioengineering, Indian Institute of Technology Bombay,Powai, Mumbai, IndiaCellular regulation is a result of complex interactions ... interactions arisingfrom DNA–protein and protein–protein binding, autoreg-ulation, and c ompartmentalization and shuttling o f regula-tory proteins. Experiments in molecular biology haveidentified ... regulatory element of the GALgenes in the nucleus. The regulatory protein may bind tothe upstream regulatory element constituting a protein–DNA interaction [4–6]. The availability of the regulatoryproteintointeractwithDNAmaydependonitsactivity,which...
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Báo cáo Y học: A b-lysine adenylating enzyme and a b-lysine binding protein involved in poly b-lysine chain assembly in nourseothricin synthesis in Streptomyces noursei pot
... Molecularcharacterization of the genes of actinomycin synthetase I and a4-methyl-3-hydroxyanthranilic acid carrier protein involved in theassembly of the acylpeptide chain of actinomycin in Streptomyces.J. ... b-lysine adenylating enzyme and a b-lysine binding protein involved in poly b-lysine chain assembly in nourseothricin synthesis in Streptomyces nourseiNicolas Grammel1,*, Kvitka Pankevych2, Julia ... activity is de®ned asnkatal, the amount of enzyme catalysing the incorporation of 1 nmol pyrophosphate into ATP per second in thepresence of b-lysine.The b-lysine binding protein was assayed in...
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Báo cáo Y học: Structural studies on the core and the O-polysaccharide repeating unit of Pseudomonas aeruginosa immunotype 1 lipopolysaccharide pot
... O5 [4,7] and 2192 [5]with two glycoforms 1 and 2 (Fig. 5A and B). As judged bythe signal intensities of the methyl groups of Rha and QuiN, in P. aeruginosa immunotype 1 glycoforms 1 and 2 arepresent ... minor O-acetyl group and phosphategroups in the core by NMR spectroscopy failed owing to ahigh degree of structural heterogeneity.The LPS was O-deacylated by mild hydrazinolysis and N-deacylated ... methylene protons of Kdo residues at d 1.9–2.2, methyl groups of 6-deoxy sugarsat d 1.26–1.35 and alanine at d 1.56,aswellasN-acetylandO-acetyl groups. Analysis of the spectrum using 2D COSYand...
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Báo cáo y học: "Strength training improves muscle quality and insulin sensitivity in Hispanic older
... trained showed increased levels of adiponectin. Interestingly, other studies investigating adiponectin levels have shown varying results. High intensity endurance training in nondiabetic individuals ... training and improved skeletal muscle insulin sensitivity. In addition, the age of participants, type of exercise training, and study design need further investigation in relation to changes in ... diabetic and non-diabetic individuals in response to 4 weeks of one-legged low-intensity strength training and reported possible mechanisms leading to a training effect including increased protein...
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Báo cáo khoa học: Chick embryo anchored alkaline phosphatase and mineralization process in vitro Influence of Ca 2+ and nature of substrates doc
... formation of anyhydroxyapatite crystals, even after 144 h incubation time, when hydrolysis of both nucleotides was completed. In contrast, the hydrolysis of AMP by GPI-bALP led to theappearance of ... formed in media lacking GPI-bALP.Mineral calcium phosphate identification by infraredspectraTo identify the quality and the type of mineral formationobtained in the mineralization medium during ... noformation of any HA after 144 h incubation time in thepresence of either ADP or ATP in hydrolysing mediumcontaining GPI-bALP and excess of calcium (to compen-sate the complexation of calcium by the...
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