... Optimization of a-acylaminoketone ecdysone agonists for control of gene expression.Bioorg Med Chem Lett 13,1883–1886.18 Tice CM, Michelotti EL & Hormann RE (2004) Ketoneligands for ecdysterone ... of 20 lL reaction volume containing 1 lL of cDNA,1 lL of each of forward and reverse sequence specific prim-ers (from 10 lm primer stock), 10 lL of supermix (Bio-RadLaboratories) and 7 lL of ... Journal compilation ª 2007 FEBS of dioxane, 40 mL of water, and 4.34 g of calcium carbon-ate. The reaction flask was placed into an 85 °C oil bath and the reaction was stirred and heated for 18...