... overproduction of B. subtilis TatAyCy, tatAyCy-strep wascutoutofpBAyCyswithNheI and XbaI and ligatedinto NheI ⁄ XbaI-cut pEXT22 to construct pEXT–AyCy.For construction of pBAD–AmiA–GFP and MdoD–GFP ... Dijl2 and Colin Robinson11 Department of Biological Sciences, University of Warwick, Coventry, UK2 Department of Medical Microbiology, University Medical Centre Groningen and University of Groningen, ... immunoblot was analysed by densitome-try and intensities of bands plotted against fraction number. Thecolumn was calibrated using a set of protein standards of knownmolecular mass, namely thyroglobin...