... from the top of the gradient and processed to analyze acid insoluble radioactivity.DNA cytofluorometryFor cytofluorometry of cellular DNA cells were trypsinized,washed with NaCl/Pi and fixed ... incubated and reoxygenated cells. The mainlynuclear localization of PCNA is due to the fixationprocedure. Acetone fixation leads to cell shrinkage and loss of membranes. Therefore cytosolic PCNA ... regulatoryphenomenon has been published for Ehrlich ascites, HeLa and CCRF cells [2,4,5]. Further cell lines examined so far,e.g.T24,A549,PC3,TC7,BHK,SW2,HL60andHUVECarealsosubjecttothefastO2-dependent...