Assessment of pretreatments and enzymatic hydrolysis of wheat straw as a sugar source for bioprocess industry

Tài liệu Báo cáo khoa học: Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as a negative regulator for N-terminal palmitoylation of Sonic hedgehog doc

Tài liệu Báo cáo khoa học: Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as a negative regulator for N-terminal palmitoylation of Sonic hedgehog doc

... Petersburg, FL, USA) followed by PCR with Taq DNA polymerase (Promega, Madison, WT, USA) using the primers 5¢-CACACTACACTGGGAAGCAGAG ACTCCAGC-3¢ and 5¢-AGCTGGCCCAGCAGCCATACACAGTTAAAG- 3¢. The cDNA was subcloned ... [43–49]. Acknowledgements The authors thank Drs Masato Yasui, Sadakazu Aiso and Masaaki Matsuoka for support; Dr Andrew P. McMahon for providing the full-length mou- se Shh...
Ngày tải lên : 18/02/2014, 16:20
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Tài liệu Wheat Straw as a Paper Fiber Source doc

Tài liệu Wheat Straw as a Paper Fiber Source doc

... wheat straw. 2.0 Wheat Straw Characterization Page 2-4 © Clean Washington Center, 1997 2.2 Mass Balances As mentioned above, Ernst and coworkers [1960] found their baled wheat straw to ... morphological plant structures contain fewer tracheids and have more ash and fine plant material. Both ash and fines are detrimental to papermaking. The material contained in r...
Ngày tải lên : 22/02/2014, 09:20
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Báo cáo khoa học: Combining theoretical analysis and experimental data generation reveals IRF9 as a crucial factor for accelerating interferon a-induced early antiviral signalling pdf

Báo cáo khoa học: Combining theoretical analysis and experimental data generation reveals IRF9 as a crucial factor for accelerating interferon a-induced early antiviral signalling pdf

... earlier maximal pathway activation and an increase in signal amplitude [3,4]. Furthermore, alterations in the activity of a kinase or phosphatase may affect the speed of signalling pathway activation. ... Tamada Y, Nakao K, Nagayama Y, Nakata K, Ichikawa T, Kawamata Y, Ishikawa H, Hamasaki K, Eguchi K & Ishii N (2002) p48 Overexpression enhances interferon-mediated expression and...
Ngày tải lên : 23/03/2014, 03:20
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Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

... genome with a GTG start. Sense primer, 5¢-GAGAGACATATGCCA TCGGCCATTTTAAAG-3¢; antisense primer, 5¢ -GAGA GAAAGCTTCTAACGCAGGGAGAAAAC-3¢. An alter- native start site would be the in-frame ATG, 30 nucleotides downstream ... incor- porate suitable restriction sites (NdeI-HindIII) into the 5¢- and 3¢ ends of the product. Sense primer, 5¢-GAGA GAGGATCCATATGACAAAAACCGAGTTAC-3¢, NdeI site; anti...
Ngày tải lên : 19/02/2014, 17:20
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Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf

Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf

... L, Hamid Q & Elias JA (2004) Acidic mammalian chitinase in asthmatic Th2 inflammation and IL-13 pathway activation. Science 304, 1678– 1682. 4 Kasprzewska A (2003) Plant chitinases ) regulation ... chitinases have catalytic domains that are at least as large as those of the plant enzymes and that may contain at least six subsites [26,30]. However, the catalytic domains of Chi...
Ngày tải lên : 07/03/2014, 11:20
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Báo cáo khoa học: Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) ppt

Báo cáo khoa học: Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) ppt

... wavelength of 280 nm and an emission wavelength of 400 nm. Adenine at concentrations ranging from 1 to 1200 pmol was used as a standard for calculation of the amount of adenine in the samples. Assay for ... Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant ( Laminaria japonica A) Ren-shui Liu 1 ,...
Ngày tải lên : 08/03/2014, 10:20
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Báo cáo khóa học: Overexpression and enzymatic characterization of Neisseria gonorrhoeae penicillin-binding protein 4 ppt

Báo cáo khóa học: Overexpression and enzymatic characterization of Neisseria gonorrhoeae penicillin-binding protein 4 ppt

... EC U ịC L EC U ịC U 2ị Enzyme activity assays $ D -Ala- D -Ala carboxypeptidase (CPase) activity was deter- mined by uorescence-based detection of D -Ala in micro- titer plate-based assays as described in detail previously [15,22]. ... of 90 min. D -Ala product was detected with QuantaBlu TM assays. j, Boc-Cbz-KAA; r, Boc-Ac-KAA; s, Ac-C-KAA; n, Ac 2 -KAA; h, Boc-H-KAA; d, Ac 2 -KA- D -...
Ngày tải lên : 23/03/2014, 12:20
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Báo cáo khoa học: X-ray crystallographic and enzymatic analyses of shikimate dehydrogenase from Staphylococcus epidermidis pot

Báo cáo khoa học: X-ray crystallographic and enzymatic analyses of shikimate dehydrogenase from Staphylococcus epidermidis pot

... 5¢-G GTGTTTTATATGGATTAAAAACAATATCACTTAC-3¢. The mutant plasmid was confirmed by DNA sequencing before transformation into E. coli strain BL21(DE3). The expression and purification of the Y211F mutant were ... such as aromatic amino acids, folic acid and ubiquinone [3]. The shikimate pathway is crucial in algae, higher plants, bacteria, apicomplexan parasites and fungi, but is absent i...
Ngày tải lên : 30/03/2014, 02:20
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