... immobilized mitochondrial
import receptor Tom70
Tom70 is an integral membrane protein consisting of a large
globular receptor domain exposed to the cytosol and a short
N-terminal transmembrane anchor. ... transformed into E. coli TG1 and
phagemid particles rescued and panned against the immo-
bilized Tom70 antigen. Four rounds of biopanning were
performed with an increase in t...
... P III and P IV) were isolated by affinity, ion
exchange and chromatofocusing chromatographies, and investigated with
respect to toxicity and sugar binding specificity. Half maximal inhibitory
concentration ... inhibitory
concentration and median lethal dose values indicate that P I and P II have
similar toxicities and that both are more toxic to cultured HeLa cells and
mice...
... curves. Squares and dotted line: data and fitting
with PHI/PHO ratio of 0.5. Triangles and continuous line: data and
fitting with PHI/PHO ratio of 1. Asterisks and broken line: data and
fitting with ... (University of Turin) for
mass spectroscopy analysis, to A. Conti and G. Giuffrida (CNR-
Torino) for N-terminal sequence determination and to D. Cavazzini
(University of...
... profile
Figure 1A shows the SDS/PAGE of purified ccNiR upon
different treatments. The complex dissociates into an intense
band of 61 kDa (NrfA) and a band of weak intensity of
19 kDa (NrfH), confirming ... nature
(Fig. 1, lane 1).
However, in the absence of boiling (Fig. 1A, lanes 2 and
4) high molecular mass bands of approximately 110 kDa
and > 200 kDa were visible, as we...
... &
Rhee, S.G. (1994) Cloning and sequencing of thiol-speci®c
antioxidant from m ammalian brain: alkyl reductases and thiol-
speci®c antioxidant de®ne a large family o f antioxidant enzymes.
Proc. N atl ... east and Arabi dopsis, and has been
shown to be thioredoxin-dependent and function in a
similar manne r to 2 Cys-Prx [ 8,9].
2Cys-Prx catalyzes, in vitro, the red uction...
... according to [56] using
BSA as a protein standard. The DNA and amino acid
sequence analyses and prediction of the molecular masses
were performed with the programs mapdraw and protean
in dnastar ... images of the trans-
formed protoplasts are shown in (A; pGFP-N
fusion) and C; pGFP-C fusion). Bright field
images of the same protoplasts were made
to visualize the protoplast’s...
... rotor (Eppendorf,
Hamburg, Germany). The supernatant was collected and
split into two equal samples: to one was added 2 lLH
2
O
and to the other was added 2 lL Endo H (0.005 U lL
)1
)
(F. Hoffmann-La ... random priming and the incorporation of [a-
32
P]dCTP.
Hybridization of the probe to the membrane and subse-
quent washes were performed as described [29]. Hybridiza-
tion w...
... struc-
ture of human and bovine MUC15, and exon skipping as a
possible explanation for the origin of the splice variant.
Interestingly, the appearance of alternative soluble variants
of membrane-associated ... node,
tonsil, and fetal liver. Furthermore, PCR screening of
bovine and human cDNA libraries showed the presence of
MUC15 mRNA in human breast, bovine mammary gland,...
... of degenerate and specific primers
and by rescreening the cDNA libraries. The partial clones
of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective
3¢ ends and included 3¢-UTRs and poly(A)-tails.
A ... with
[
32
P]ATP[cP] and T4 polynucleotide kinase. The labeled
oligomers were annealed by heating to 90 °C an equimolar
mixture of the upper and lower strands in r...