... thatm32,2,7GpppA and m27,3¢-OGpppG compete for thesame binding site in the inhibitory experiment [26].The kinetic parameters obtained for m27,2¢-OGpppG and m27,3¢-OGpppG indicate that the 2¢-OH and ... parameters obtained for m27,2¢-OGpppG and m27,3¢-OGpppG demonstrated the crucial role ofthe 2¢-OH and 3¢-OH groups of the m7Guo moiety for C. elegans DcpS hydrolysis. The 2¢-O-Me and 3¢-O-Me0 ... exists in five different iso-forms, with different affinity to m7GpppG and m32,2,7GpppG [20,21]. Human and yeast DcpS caneffectively hydrolyze only the m7GpppG cap, and human DcpS has activity...