... samplesand Dr. Andrew Gooley and Prof. Keith Williams for their support.References1. Yan, J. X., Wilkins, M .R., Ou, K., Gooley, A. A., Williams, K. L., Sanchez, J.-C.,Golaz, O., Pasquali, C., and ... HPLC system with a fluo-rescent detector and gradient controller is available.Acid hydrolysis is the first and most important step to release the amino acids fromthe proteins, and it must be carefully ... caramelized and further charcoaled, and the acid hydrolysis results in ablack residue. This residue appears to precipitate protein/amino acids, interferes with114 Yan and Packerchromatography, and leads...