... 70 mL of glycerol.8. Poly-L-lysine-coated slides: slides are immersed in 0.01% (w/v) poly-L-lysine for 5 minand dried at room temperature.3. Methods3.1. Observation and Measurementof Mucous Thickness ... rat, frog and human gastric mucosa: influence of feedingprostaglandin, N-acetyl-cysteine and other agents. Clin. Sci. 63, 187–195.4. Bell, A. E., Allen, A., Morris, E. R., and Ross-Murphy, S. ... them in a 1–cm2sand-62 Allen and Pearsonwich of a suitable piece of tissue. Pig’s liver is satisfactory for this procedure. Thesandwiched biopsies are immediately snap-frozen and stored at –20°C....