... Construction of rat BMP4 and BMP6 recombinant adenoviruses The AdEasy Vector System [18] was used for construction of the rat BMP4 and BMP6 adenoviral vectors The linear pShuttle-rBMPs and the pAdEasy plasmid ... formation in rats We studied ectopic bone formation in 2-monthold male rats Eight AN rats and 20 SD rats were used for the study of BMP4, and 16 AN rats and 21 SD rats for the study of BMP6 Animal ... titers of the purified [E1-,E2b-]ADGFP&hBMP4, [E1-,E2b-]ADhBMP6, and [E1-,E2b-]ADGFP&hBMP6 were 8.6 × 1011, 9.6 × 1011, and 3.6 × 1011 particles/ml, respectively The amounts of human BMP4 and BMP6...