... (L5D2), day last instar (L6D0), and day last instar (L6D1) larvae (Inset) DDC activity in integuments from the same larval stages a* , Significantly different from TH activity of L5D2 larval dorsal integument ... counter (Aloka LSC5100, Tokyo, Japan) Cloning and sequence analysis of TH cDNA Total RNA was isolated from integuments of day last instar larvae using TRIzol reagent (Gibco-BRL) according to the manufacturer’s ... (5¢-CAGCTGCCCAGAAGAACCGCGAGA TG-3¢, +11 to +36; and 5¢-GAACTCCACGGTGAACC AGT-3¢, +1286 to +1305 bp), DDC-specific primer pair (5¢-ATGGAGGCCGGAGATTTCAAAG-3¢, +1 to +22 bp; and 5¢-ACGGGCTTTAAGTATTTCATCAGGC-3¢,...