... homologous liver zebrafish protein, where the introduction of a disulfide bridge resulted, intriguingly, in a singly ligated protein, with the cholate occupying the more superficial binding site [16] ... lead to distinct binding and functional properties [22,23] In line with this, we have shown here that the presence of a disulfide bridge, while maintaining the same binding stoichiometry, induces ... activation regulates ligand binding in chicken liver bile acid-binding protein J Biol Chem 281, 9697–9709 10 Eberini I, Guerini Rocco A, Ientile AR, Baptista AM, Gianazza E, Tomaselli S, Molinari...