... article as: Kadow et al.: Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007 AMB Express 2011 ... amount of enzyme that catalyzes the oxidation of μmol of substrate per minute Results Cloning, expression and purification of 2, 5-diketocamphane 1,2-monooxygenase The 2,5-diketocamphane 1,2-monooxygenase ... Page of Figure Vector 2,5-DKCMO_pET-28 for expression of recombinant 2,5-DKCMO from P putida NCIMB 10007 under control of T7 promoter in E coli BL21 The 2,5-DKCMO-gene was introduced using the...