Metabolic Engineering Of Saccharomyces Cerevisiae For Sesquiterpene Production Gionata Scalcinati
Tài liệu Báo cáo khoa học: Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as a negative regulator for N-terminal palmitoylation of Sonic hedgehog doc
... 5¢-CACACTACACTGGGAAGCAGAGACTCCAGC-3¢ and 5¢-AGCTGGCCCAGCAGCCATACACAGTTAAAG3¢ The cDNA was subcloned into the EcoRV site of pBluescript SK(+) (Stratagene, La Jolla, CA, USA) and sequenced using an automated ... Na -palmitoylation of Gas is regulated also remains unclear However, we assume that mammalian Gup1 competes with Skn for Shh to prevent palmitoylation rather than catalyzing depalmitoylation of ... buffer, and the eluted samples were subjected to western blot analysis as described above The authors thank Drs Masato Yasui, Sadakazu Aiso and Masaaki Matsuoka for support; Dr Andrew P McMahon for...
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Báo cáo khoa học: Energetic and metabolic transient response of Saccharomyces cerevisiae to benzoic acid docx
... properties and cell size to the presence of benzoic acid Transient response to benzoic acid total membrane surface area (=AXÆCXÆVL) available for the benzoic acid transport during the transient ... al Transient response to benzoic acid A B C D E Fig Transient responses to the shift in benzoic acid concentration (the timing of the shift is marked by a dashed vertical line) (A) Benzoic acid ... consumption due to the addition of benzoic acid In order to study the adaptation of cells to benzoic acid, we use the transient O2 consumption profile to reconstitute the dynamics in benzoic acid transport,...
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Tài liệu Báo cáo khoa học: Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae docx
... the most reliable means of purifying integral membrane proteins from the mitochondrial outer membrane, and of the 11 most abundant integral proteins, 10 had a-helical transmembrane segments Of ... the proteins of the outer membrane, we set out to determine what proportion was integral and to identify the major protein species To determine whether the major outer membrane proteins were integral ... assembly of beta-barrel membrane proteins J Biol Chem 276, 32399–32402 Integral proteins in the mitochondrial outer membrane Tokuda H & Matsuyama S (2004) Sorting of lipoproteins to the outer membrane...
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Tài liệu Báo cáo Y học: Targeting of malate synthase 1 to the peroxisomes of Saccharomyces cerevisiae cells depends on growth on oleic acid medium pptx
... Application of the antibody to thin sections of wild-type cells grown on oleic acid medium Fig SKL is required to direct Mls1p to the peroxisomes under oleic acid- medium conditions (A) Speci®city of the ... issue of the partitioning of the glyoxylate cycle in cells grown under fatty acid- medium conditions has hitherto remained open We showed here that one of the key glyoxylate-cycle enzymes, Mls1p, ... location of the glyoxylate cycle in yeast grown on carbon sources other than fatty acids The only other key enzyme unique to the glyoxylate cycle, Icl1p, is also extra-peroxisomal [4], as are the...
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Báo cáo khoa học: Mutants of Saccharomyces cerevisiae deficient in acyl-CoA synthetases secrete fatty acids due to interrupted fatty acid recycling docx
... certain pools of fatty acids To assess the role of acyl-CoA synthetases in regulating particular pools of fatty acids, we used mutants of S cerevisiae characterized by various combinations of deletions ... exogenous fatty acids, additional release of internal fatty acids had to contribute to the increase of intracellular fatty acids However, analysis of the previously mentioned fatty acid specificity of ... (square)] (D) Concentration of fatty acids in the pools of free fatty acids in the media (diamonds), of free fatty acids in the cells (square) and of esterified fatty acids (triangle) The error...
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Báo cáo khoa học: Increased NADPH concentration obtained by metabolic engineering of the pentose phosphate pathway in Aspergillus niger docx
... (PLS) regressions For future metabolic engineering of strains with the purpose of obtaining increased NADPH levels and for the understanding of the regulation of the NADPH level it is important ... acid-producing mycelia [26] Overproduction of 6PGDH resulted in an increase of synthesis of pyridine nucleotide cofactors, as the total pool of these increased Whether the synthesis is regulated by the ... might be increased by metabolic pathway engineering, e.g overproduction of enzymes in the PPP or deletion of genes in glycolysis when a hexose is the carbon source NADPH is produced in two of the...
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Báo cáo Y học: Expression of the V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saccharomyces cerevisiae and study of its complementation pdf
... AACEVAPD1 AACEVAPD3 AACEVAPD2 AACEVAPD5 AACEVAPD4 AACEVAPD6 Vma3p Vma11p Vma16p 100 97 80 80 80 80 50 50 32 100 94 93 93 54 51 28 AACEVAPD5 AACEVAPD2 AACEVAPD3 AACEVAPD1 Table Alignments of the ... R Hirata of the Institute of Physical and Chemical Research (Wako, Japan), and the antibody against the 100-kDa (a) subunit of S cerevisiae V-ATPase was purchased from Molecular Probes Inc., Eugene, ... preparation of recombinants in yeast expression vector In the case of AACEVAPD1, and 6, TAA is used as an Acetabularia- specific codon usage (translated as Gln) Conversion of TAA to CAA was performed by...
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Báo cáo khoa học: Lipins from plants are phosphatidate phosphatases that restore lipid synthesis in apah1Dmutant strain of Saccharomyces cerevisiae ppt
... PAHs are orthologs of yeast PAH1 and mammalian lipins Based on previous studies demonstrating the involvement of yeast PAH1 and mammalian lipins in storage lipid accumulation [27], we were initially ... derepression of key enzymes involved in phospholipid (PL) biosynthesis [20,21] In mammals, highly phosphorylated forms of Lipin-1 are enriched in the cytoplasm, whereas dephosphorylated forms are found ... homologs of PAH1 0.4 0.08 0.04 0.3 0.2 0.1 0.0 Fig Mutations within N-LIP and C-LIP domains in uence the ability of plant PAH1 to restore TAG synthesis in yeast pah1D strain (A) TAG ⁄ PL ratio of the...
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Báo cáo khoa học: Schemes of flux control in a model of Saccharomyces cerevisiae glycolysis docx
... investigate patterns of flux control The model with the best-fit Vmax parameters was used as the base model for parameter scanning using routines contained in GEPASI METHODS Model A model of branched glycolysis, ... in all combinations) using the parameter scanning functions of GEPASI Only around 50% of the simulations reached steady state, and of those that did a single step was usually seen to dominate flux ... of metabolite concentration and flux are obtained from populations of yeast cells and so reflect an aggregate of the states of many individual organisms Although no fitted model in this paper individually...
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Báo cáo khoa học: Mutational analysis of functional domains in Mrs2p, the mitochondrial Mg2+ channel protein of Saccharomyces cerevisiae ppt
... either are involved in oligomerization of the Mrs2p channel protein or in forming structures participating in the gating of this channel Possibly they contribute to both functions The coiled-coil ... formation of higher order structures of the Mrs2p part on the inner side of the membrane, which may contribute to the proposed opening ⁄ closing of the channel The Mrs2 sequence C-terminal to the TM domains ... for the correct integration of the protein into the inner -mitochondrial membrane [3,8] Substitution of these glutamic acids by lysines (positively charged) resulted in a complete loss of mitochondrial...
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Báo cáo khoa học: Activation of the plasma membrane H+-ATPase of Saccharomyces cerevisiae by glucose is mediated by dissociation of the H+-ATPase–acetylated tubulin complex docx
... al Dissociation of tubulin H+-ATPase complex by glucose H+-ATPase activity of isolated plasma membrane is inhibited by tubulin Plasma membranes isolated from S cerevisiae were incubated in the ... hydrophobic tubulin partitioning into the detergent phase (HAT) is the acetylated tubulin forming a complex with H+-ATPase The finding of the H+-ATPase acetylated tubulin complex in plasma membranes of glucose- starved ... some part of the H+-ATPase, reinforcing the idea of a complex between H+-ATPase and acetylated tubulin at the plasma membrane This complex was dissociated by pretreatment of cells with glucose...
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Báo cáo khoa học: The N-acetylglutamate synthase/N-acetylglutamate kinase metabolon of Saccharomyces cerevisiae allows co-ordinated feedback regulation of the first two steps in arginine biosynthesis potx
... F99L kinase, suggests a mechanism of co-ordinated feedback regulation of the synthase and the kinase Arginine feedback inhibition of N-acetylglutamate kinase is altered in the absence of N-acetylglutamate ... expressing the kinase protein) allow growth of the argininedeficient mutant in the absence of arginine On the other hand, plasmids pYK7 and the empty vector pTrc99a (both lacking the yeast kinase ... kinase activity in cell extracts was assayed in the presence of increasing arginine concentrations Figure 5A compares the arginine inhibition curves of the wild-type and F99L mutant kinases The...
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Báo cáo Y học: Molecular characterization of MRG19 of Saccharomyces cerevisiae Implication in the regulation of galactose and nonfermentable carbon source utilization pdf
... containing galactose as a carbon source Wild-type and mrg19 strains grew on galactose plates within 12 h while gal3 strain showed the characteristic delay in growth on galactose Interestingly, the ... disruption of MRG19 might effect GAL induction only at low galactose concentrations This hypothesis was tested by monitoring galactokinase induction as a function of time in the wild-type and in the MRG19 ... metabolize the carbon source (Fig 6, Compare and or and 4) However, an increase of 50% in the rate of oxygen uptake was observed in the MRG19 disruptant as compared with the wild-type in the absence of...
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Báo cáo khoa học: ISC1-encoded inositol phosphosphingolipid phospholipase C is involved in Na+/Li+ halotolerance of Saccharomyces cerevisiae pptx
... references isc1D + pDZ6 was a transformant of the isc1D mutant with the ISC1 containing plasmid, pDZ6 cell homogenate The characteristics of the Disc1::HIS5 deletion strain, DZY1, which was constructed ... sphingolipids in the heat stress response of Saccharomyces cerevisiae J Biol Chem 272, 32566–32572 14 Wells, G.B., Dickson, R .C & Lester, R.L (1998) Heat-induced elevation of ceramide in Saccharomyces cerevisiae ... distinct phospholipid cofactors for full activity [1] Hence, not only the bioactive messenger produced by Isc1p and the mechanism of its action but also the salt-sensing process involving Isc1p...
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Từ khóa: succinate dehydrogenase of saccharomyces cerevisiae the unique enzyme of tca cycle current kengineering of affibody molecules for therapy and diagnosticsuse of e coli for the production of a single proteinestablishment of standard protocols for transient production of recombinant proteinsmetabolic map of grr1δ cells grown on 2 glucose previous page gene and protein expression measurements were overlaid onto a metabolic map of s cerevisiae metabolism enzymes are represented by nodes with the color of the node denotmetabolic engineering for improvement of productivitygenetic engineering of the plant cell factory for secondary metabolite production indole alkaloid production in catharanthus roseus as a modelethanol production during batch fermentation with saccharomyces cerevisiaeprocess design for the production of ethanol from ethylenechemical equation for the production of ethanol from etheneword equation for the production of ethanol from ethenestatement of purpose sample for phd electrical engineeringstatement of purpose examples for graduate school engineeringlist of major projects for electrical and electronics engineeringlist of minor projects for electrical and electronics engineeringNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDENghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Tổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Kiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)Chiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015TÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ