... labeling at any temperature (Fig 5A) A similar fast activation was observed for Y2 receptors of rat forebrain cells (not shown) This was contrasted by a gradual, steady accumulation of surface Y1 sites ... in a- actinins, adaptins and selectins A role for single intramembrane thiols could also be assumed, based on the lack of Y2 activation by MTSET, and limited activation by NEM, an alkylator that ... gpY2-CHO monolayers or particulates, and to dispersed rat forebrain cells The labeled Y2 agonist was input at 50 pM in all cases For assay details see Methods All data, shown ± SEM, are averages...