... 8521– 8525 Panetta G, Arcovito A, Morea V, Bellelli A & Miele AE (2008) Hb([alpha][alpha],[beta][beta]): a novel fusion construct for a dimeric, four-domain hemoglobin Biochim Biophys Acta Proteins ... in addition to cellular damage such as lipid peroxidation, protein oxidation, and DNA damage [21,22] Hb may generate reactive oxygen species, which can be particularly deleterious in clinical applications, ... properties excess of catalase (Roche, Mannheim, Germany) was added to remove residual H2O2, after which mm sodium sulfide was added; finally, the absorbance at 620 nm (A6 20 nm) was measured Measurement...