... kinase and mM MgCl2 or 0.05 UÆmL)1 of pyruvate kinase and mM MnCl2 PEP carboxylase activity was determined in a coupled assay with malate dehydrogenase, as described previously [30] The rate of ... transiently phosphorylated in the course of the catalytic cycle, for instance, the activesite histidines of enzyme I of the PTS [2], phosphoenolpyruvate synthase, and pyruvate phosphate dikinase, ... kinase/L-lactate dehydrogenase assay Obviously, this methodology was not applied with compounds 1b–e (also 1f and 2f when Mn2+ was present) as they are good inhibitors of pyruvate kinase In these cases the...