... following lengthy incubation periods However, synthetic prions were not able to transmit disease directly to wild type mice To obtain infectivity in wild type mice, synthetic prions were Int J ... appears that infectivity of serial PMCA was due to newly synthesized PrPSc since the original PrPSc seeds were diluted beyond the minimum infectious level Although infectious, the in vitro generated ... strain properties of brain-derived PrPSc appeared to be conserved in the PMCA product by exhibiting indistinguishable clinical signs and vacuolation pattern In addition, the pathogenecity of in...