... Precast, 4–20% Tris-HCl Ready Gel (Bio-Rad, Hercules, CA) for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) Silver stain kit (Bio-Rad) Coomasie blue R-250 (Sigma, St Louis, ... Methanol (v/v) 3-kDa Cutoff centrifugal ultrafilters (Filtron, Northborough, MA) Precast, 4% and 4-2 0% Tris-glycine gels for SDS-PAGE (Novex, San Diego, CA) Kaleidoscope™ (Bio-Rad) and See-Blue ™ (Novex) ... Diurnal variations in tear glycoproteins Evidence for an epithelial origin for the major non-reducible ≥ 450 kDa sialoglycoprotein(s) Curr Eye Res 16, 577–588 Mucin-Bacterial Binding Assays 437...