... Strategies for mapping large regions of mammalian genomes, in Pulsed-Field Gel Electrophoresis : Protocols, Methods and Theories, Methods in Molecular Biology, vol 12 (Burmeister, M., and Vlanovsky, ... solution: 890 mM Tris-HCl, pH 8.3, 890 mM boric acid, and 2.5 mM EDTA CHEF apparatus (noncommercial apparatus [4]) Size markers: λ4-phage concatemers and chromosomes of S cerevisiae (22 5-2 200 kb and ... in the presence of 5-azacytidine as methylation inhibitor 10 11 12 13 14 RPMI 1640 medium (Gibco-BRL) J Prep medium (J Bio, Les Ullis, France) Low melting point agarose (Bio-Rad) Lysis buffer:...