... however, that including reversibility in reactions with high Keq requires the tting and some times the guessing of kinetic parameters that cannot be easily determined (Km for products, Vm in the reverse ... determined in amoebal 200:NIH strain [36] Some kinetic data for amoebal NAD(P)H-ADHs has been described [44] These reactions were included as an irreversible bisubstrate reaction, also involving ... further renement of the present kinetic glycolysis model Then, the available in vitro kinetics did not fully account for the in vivo observed behavior However, by xing the PPi concentration, the...