... of mucin glycoproteins The hydrolysate of mucin is then derivatized with Fmoc on the α-amino groups under alkaline conditions The derivatized amino acids are separated by a C18 reversed-phase column ... continuous flow of helium Hypersil C18 reversed-phase column: 150 × 4.6 mm inner diameter µm (Keystone, Bellefonte, PA) In-line filter (2 µm) (Upchurch, cat no 10 0-1 0) 2.2 Chemicals 2.2.1 Hydrolysis and ... Wilkins, M R., Ou, K., Gooley, A A., Williams, K L., Sanchez, J.-C., Golaz, O., Pasquali, C., and Hochstrasser, D.F (1996) Large-scale amino-acid analysis for proteome studies J Chromatogr 736, 291–302...