Mass Spectrometry A Textbook, 2nd Edition Jurgen H. Gross

Insights into protein kinase a activation using cAMP analogs and amide h 2h exchange mass spectrometry

Insights into protein kinase a activation using cAMP analogs and amide h 2h exchange mass spectrometry
... Insights into Protein Kinase A Activation using cAMP Analogs and Amide H/ 2H Exchange Mass Spectrometry TANUSHREE BISHNOI A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT ... was coupled with 8-AEA -cAMP through a spacer arm A bed volume of ml of the resin was taken in a 50 ml tube The resin was reactivated by three alternative washes with High pH Buffer (200mM ethanolamine, ... tested, however Rp-cAMPS (Rp-adenosine 3’,5’-cyclic monophosphorothioate) and related derivatives are the only known cAMP analogues that act as antagonists and competitive inhibitors of cAMP mediated...
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Tài liệu Báo cáo khoa học: A functional polymorphism of apolipoprotein C1 detected by mass spectrometry docx

Tài liệu Báo cáo khoa học: A functional polymorphism of apolipoprotein C1 detected by mass spectrometry docx
... ethnic background of at least 1000 subjects was typical of the American Midwest with  90% of European ancestry and  5% each of African and Asian ancestry An exception was the targeted analysis of ... variants of ApoC1 A functional importance of a methyl group side chain at position 45 was also suggested by homology alignment ApoC1 from six available species shows either Ala or Thr at the comparable ... sum of peaks of ApoC1 was determined for fraction and assigned a value of 1.0 The peak ratios in subsequent fractions are expressed relative to that value Also shown are the relative ratios of...
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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc

Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
... His-Tagged Ssa1p (lanes 8–14); a mixture of untreated Ure2p and Ssa1p is seen in lanes and 10 Ure2p, Ssa1p and Ure2p incubated with Ssa1p treated with BS2G are seen in lanes and 8, and 11 and and 13, ... a region, located within the C-terminal domain of Ure2p that interacts with Ssa1p Because the C-terminal domain of Ure2p is tightly involved in the assembly of the prion into fibrils [25–28] and ... (A) or western blotted and stained with antibodies directed against Ure2p or His-Tagged Ssa1p (B) (A) A mixture of untreated Ure2p and Ssa1p (lane 1); Ure2p alone (lanes 2, 5, and 11), Ssa1p alone...
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Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx

Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx
... ATTGTCCGGGGTTGTTCGCAACGTACAA TTGTACGTTGCAATCAACCCCGGACAAT ATTGTCCGGGGTTGATTGCAACGTACAA ACCACAGGTGTTGTACATTGCGATCAACC GGTTGATCGCAATGTACAACACCTGTGGT TCCTACCGCAGGTCCTGAGCAGCAGGGA TCCCTGCTGCTCAGGACCTGCGGTAGGA TTTGCCCGCACTGGCGGCTTTGCGGTGTC ... TTTGCCCGCACTGGCGGCTTTGCGGTGTC GACACCGCAAAGCCGCCAGTGCGGGCAAA TTGGCATACGTACCACAGGTGTTGTAC GTACAACACCTCCGGTACGTATGCCAA Change Change Change Change Change Change Change Change Change Change Change Change ... role as the catalytic base (c) D75E: aspartic and glutamic acids are dicarboxylic acids, but glutamic acid has an additional carbon in the lateral chain The clear effect of this change in the RNase...
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Báo cáo khoa học: The changing patterns of covalent active site occupancy during catalysis on a modular polyketide synthase multienzyme revealed by ion-trap mass spectrometry pptx

Báo cáo khoa học: The changing patterns of covalent active site occupancy during catalysis on a modular polyketide synthase multienzyme revealed by ion-trap mass spectrometry pptx
... 55 N A N A 0a 0a N A N A 58 4 9a 2 8a a Methylmalonyl-CoA Malonyl-CoA Propionyl-CoA; methylmalonyl-CoA; NADPH Butyryl-CoA; methylmalonyl-CoA; NADPH Valeryl–CoA; methylmalonyl-CoA; NADPH Propionyl-CoA; ... chain-extension AT and ACP domains was considerably less than 100% As the AT-catalyzed reaction is readily reversible, the extent of loading of methylmalonate on the AT and ACP domains is probably ... changes during catalysis [22,23] Both animal FAS and modular PKS are functional homodimers, which raises additional questions about the interactions between the active sites of an identical pair of...
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