Biomass modelling: Estimating thermodynamic properties from the elemental composition

Tài liệu Báo cáo khoa học: Properties of the recombinant glucose⁄galactose dehydrogenase from the extreme thermoacidophile, Picrophilus torridus ppt

Tài liệu Báo cáo khoa học: Properties of the recombinant glucose⁄galactose dehydrogenase from the extreme thermoacidophile, Picrophilus torridus ppt
... at the bottom of the cleft and a lobe containing the structural zinc at the mouth of the cleft and thus exposed to the solvent [8] Gradual depletion of the enzyme first of the structural and then ... expression of the glucose dehydrogenase gene of P torridus in Escherichia coli, the biochemical characterization of its product and the effect of zinc ions on the pH and temperature stability of the ... to unravel the physiological roles of these enzymes in P torridus The results from testing the substrate specificity of the purified recombinant GdhA indicate a relatively strict range of substrates...
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Tài liệu Báo cáo khoa học: Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentre redox protein pptx

Tài liệu Báo cáo khoa học: Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentre redox protein pptx
... using the residual water signal as internal reference [49] Modelling the thermodynamic parameters The model used for the thermodynamic characterization of DvHc3 [13] was applied to the data obtained ... solutions In the reduced and intermediate 2257 Thermodynamic parameters in ligated proteins stages of oxidation the pH was adjusted inside the anaerobic glove box with argon circulation to avoid the ... potential of the centres is small [40,41], and also with the experimental observation for monohaem cytochromes that the effect of anion binding or complex formation on the reduction potential is small...
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Tài liệu Báo cáo Y học: Electrochemical, FT-IR and UV/VIS spectroscopic properties of the caa3 oxidase from T. thermophilus docx

Tài liệu Báo cáo Y học: Electrochemical, FT-IR and UV/VIS spectroscopic properties of the caa3 oxidase from T. thermophilus docx
... UV-VIS and FT-IR spectroscopic properties of the caa 3oxidase from T thermophilus in the presence and absence of cyanide, and compare the observed properties to previous reports on members of the heme ... between cytochrome c and cytochrome c oxidase Previous reports on the caa3- oxidase from T thermophilus concluded that this enzyme is a typical member of the heme copper oxidase family [12], with the ... copper oxidase family such as cytochrome c oxidase from bovine heart and P denitrificans, and the aberrant ba3 -oxidase from T thermophilus MATERIALS AND METHODS Sample preparation The caa3- type cytochrome...
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Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc

Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc
... histidines (His67 and His247, presumably providing metal binding at site II and the neighbouring His242) bind phosphate ions, thereby providing another level of competition for metal ion binding Kinetics ... modelling of Cu(II) and Ni(II) transport by albumins On the other hand, the direct thermodynamic and kinetic characterization of Ni(II) binding at site I in HSA and BSA was obtained These data ... distinctly weaker than the model peptides A very strong inuence of phosphate ions on Cu(II) and Ni(II) binding at site II, as well as on kinetics of Ni(II) binding and substitution by Cu(II) at site...
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COMBINING EVIDENCE ON AIR POLLUTION AND DAILY MORTALITY FROM THE 20 LARGEST US CITIES: A HIERARCHICAL MODELLING STRATEGY potx

COMBINING EVIDENCE ON AIR POLLUTION AND DAILY MORTALITY FROM THE 20 LARGEST US CITIES: A HIERARCHICAL MODELLING STRATEGY potx
... structure and prior distributions Description of the databases The analysis database included mortality, weather and air pollution data for the 20 largest metropolitan areas in the USA for the 7-year ... average, maximum and 8-h maximum, we used the 24-h mean for each day If a city has more than one weatherstation, we took the average of the measurements from all available stations The PM10 and ozone ... used to estimate the air pollution mortality relative rate separately for each location, accounting for age-speci®c longer-term trends, weather and Air Pollution and Mortality 267 Table Summary...
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Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf

Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
... exposed to the solvent (Fig S5B) Resolution of the structure of NADP+-bound FeR revealed that the nicotinamide moiety of NADP+ faces the re-side of the isoalloxazine ring, and that the 2Â-P-AMP ... claimed that His126 of A fulgidus FeR is one of the candidates for the ferric ion-binding residue based on the structure of the mercury-bound derivative, although a ferric ion was not found at the ... atoms Two of these are close to the FMN binding site, and the other is on the opposite surface of the protein An anomalous-difference map calculated from the native dataset showed signicant FEBS Journal...
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Báo cáo khoa học: Structural and thermodynamic insights into the binding mode of five novel inhibitors of lumazine synthase from Mycobacterium tuberculosis pptx

Báo cáo khoa học: Structural and thermodynamic insights into the binding mode of five novel inhibitors of lumazine synthase from Mycobacterium tuberculosis pptx
... the same conditions, these apparent values can be used for comparison of binding afnities of the inhibitors under study The tting of the binding isotherms of all ve compounds with a binding model ... nitrogen and main and side-chain oxygen atoms of Ala59 and Glu61 of one subunit and with the main chain nitrogen of Asn114Â of the other subunit The contacts of the ribityl chain to His89 and Lys138Â ... al Lumazine synthase from M tuberculosis become increasingly difcult because of the growing antibiotic resistance of M tuberculosis The elucidation of the complete genomes of M tuberculosis and...
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Báo cáo Y học: Spectroscopic characterization and ligand-binding properties of chlorite dismutase from the chlorate respiring bacterial strain GR-1 ppt

Báo cáo Y học: Spectroscopic characterization and ligand-binding properties of chlorite dismutase from the chlorate respiring bacterial strain GR-1 ppt
... resonance study of the role of nitric oxide and catalase in the activation of guanylate cyclase by sodium azide and hydroxylamine Modulation of enzyme responses by heme proteins and their nitrosyl derivatives ... progress The evidence from the spectroscopic and ligand-binding studies of chlorite dismutase suggest binding of chlorite to the five-co-ordinate high-spin ferric form of the enzyme as the first step of ... we have found chlorite dismutase to be easily accessible to exogenous ligands DISCUSSION Spectroscopic properties of chlorite dismutase The EPR spectroscopic properties of chlorite dismutase have...
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Báo cáo khóa học: Functional properties of the protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus A member of a novel protein family related to protein disulfide-isomerase doc

Báo cáo khóa học: Functional properties of the protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus A member of a novel protein family related to protein disulfide-isomerase doc
... tokodaii; Ap, Aeropyrum pernix; Ta, Thermoplasma acidophilum; Tv, Thermoplasma volcanium; Fa, Ferroplasma acidarmanus; Tm, Thermotoga maritima; Aa, Aquifex aeolicus; Tt, Thermoanaerobacter tengcongensis ... presence of unlabeled ATP, indicating that ATP and the analog 8-azido-ATP recognize the same binding site The ATPase activity of PfPDO was demonstrated The hydrolysis of ATP was linear for up to 30 at ... understanding of the function of these proteins in hyperthermophilic archaea and bacteria In fact, PfPDO is able to catalyse the oxidation of dithiols, as well as the reduction and rearrangement of...
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Báo cáo Y học: The membrane-bound [NiFe]-hydrogenase (Ech) from Methanosarcina barkeri : unusual properties of the iron-sulphur clusters docx

Báo cáo Y học: The membrane-bound [NiFe]-hydrogenase (Ech) from Methanosarcina barkeri : unusual properties of the iron-sulphur clusters docx
... properties of [NiFe]-hydrogenases [27,41] The interaction of H2 with hydrogenases offers the possibility to study redox changes in enzyme in the absence of redox mediators simply by varying the H2-partial ... are not suited for the study of the Fe–S clusters of Ech As the membranes contain only very low amounts of other hydrogenases, the Niu* signal is considered to be due to Ech only; its concentration ... titration in the absence of these dyes (Fig 9) The g ¼ 2.01 signal found in the absence of dyes was not detectable in the EPR spectra in the presence of dyes as it was obscured by the strong radical...
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Báo cáo Y học: The Fe-only nitrogenase and the Mo nitrogenase from Rhodobacter capsulatus A comparative study on the redox properties of the metal clusters present in the dinitrogenase components doc

Báo cáo Y học: The Fe-only nitrogenase and the Mo nitrogenase from Rhodobacter capsulatus A comparative study on the redox properties of the metal clusters present in the dinitrogenase components doc
... signal The intensity of this signal strongly varied from preparation to preparation A decrease in specific activity was always accompanied by an increase in signal intensity, suggesting that the ... characterization of the Fe-only nitrogenase isolated from R capsulatus, which included a detailed comparison with the Mo- containing nitrogenase from the same organism, showed that: (a) the Fe nitrogenase ... performed as described by Schneider et al [8] In view of the difficulty in separating the dinitrogenase (Rc 1Mo) and dinitrogenase reductase component (Rc 2Mo) of the Mo nitrogenase from R capsulatus by...
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Báo cáo khoa học: Enzymatic properties of the lactate dehydrogenase enzyme from Plasmodium falciparum pdf

Báo cáo khoa học: Enzymatic properties of the lactate dehydrogenase enzyme from Plasmodium falciparum pdf
... polarity of the environment of the fluorophore In the case of PfLDH, the signal change on binding to the active site was too small to be used as a reliable reporter of the formation of the binary ... that the KM is controlled by a single ionizing group with a pK of 7.95 ± 0.08, similar to other lactate dehydrogenases of this mechanistic family [9] Transient kinetic properties of the enzyme: the ... concentrations of either substrate or cofactor with varied concentrations of the other The experiments were performed at different, fixed concentrations of either lactate or NAD+ A subsequent set of experiments...
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Báo cáo khoa học: Thermodynamic analysis of the unfolding and stability of the dimeric DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima and its E34D mutant pdf

Báo cáo khoa học: Thermodynamic analysis of the unfolding and stability of the dimeric DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima and its E34D mutant pdf
... studied the thermal unfolding of both the wild-type HUTmar protein and its E34D mutant in acidic solutions (pH 4.00, 3.75 and 3.50) to improve the reversibility of unfolding and avoid postunfolding ... We report here the results of an extensive thermalstability study of a hyperthermophilic protein, the histonelike protein from T maritima (HUTmar-wt) and its E34D mutant, carried out using differential ... represented by the empiric equation: Fig The temperature dependence of the unfolding heat effect for the HUTmar proteins The solid line corresponds to the common DHU(T) for HUTmar-wt and its E34D mutant...
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Báo cáo khoa học: Purification and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf

Báo cáo khoa học: Purification and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf
... liver and four other organs of the spadefoot toad Scaphiopus couchii [19] In the present study, two GST isoforms were purified from liver of the anoxia tolerant turtle, T s elegans Analysis of kinetic ... hydroperoxides and aldehydes These may be the primary substrates of the microsomal or membrane-bound GST in the same way as they are the substrates for Se-dependent glutathione peroxidases (the ‘classical’ ... Using the standard curve constructed from the protein standards (Fig 4B), the molecular mass of the Peak GST subunit was determined to be 34.0 kDa (Table 2) Peak showed subunits of 36.8 and 32.6...
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Báo cáo khoa học: H NMR study of the molecular structure and magnetic properties of the active site for the cyanomet complex of O2-avid hemoglobin from the trematode Paramphistomum epiclitum pdf

Báo cáo khoa học: H NMR study of the molecular structure and magnetic properties of the active site for the cyanomet complex of O2-avid hemoglobin from the trematode Paramphistomum epiclitum pdf
... CbHs CdHs CeHs CfH NH CaH CbH CdHs CeHs NH CaH CbH CdH NH CaH CbH CdHs CeHs CfH NH CaH CbH CbHÂ CdHs CeHs OH NH CaH CbH3 NH CaH CbH CcH3 NH CaH CbH CbHÂ CcH CdH3 CdH3Â NH CaH CbH3 NH CaH CbH ... NH CaH CbH CbHÂ NdH NH CaH CbH CcH3 NH CaH CbH CcH3 CcH3Â NH CaH CbH CbH CdHs CfH NH CaH CaHÂ NH CbH CbHÂ CdHs CeHs CfH NH CaH CbH CdHs CeHs CfH NH CaH CbHs CcH CeH3 CaH CbH CcHs CcHÂ CdH3 3.25 ... position E7 as the source of the H- bond to ligand on the basis of a partial sequence, which had indicated a Tyr on the distal E-helix The similarity in the 1H NMR spectra of the three globin complexes...
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