Adaptive evolution of a duplicated pancreatic ribonuclease gene in a leafeating monkey
Adaptive evolution of a duplicated pancreatic ribonuclease gene in a leafeating monkey
... Enzyme activities of recombinant RNASE1B, RNASE1 and mutant forms of RNASE1 a, RNase activity against yeast tRNA at different pH levels b, RNase activity against dsRNA Mutant forms of douc langur ... 2.5 RNase activity against dsRNA (OD/min/nmol) Using statistical analysis of nucleotide substitutions and biochemical assays of recombinant proteins, we have described the adaptive evolution of ... function because the paralogous RNASE1 retains it; it is likely that some of the adaptive charge-altering substitutions in RNASE1B are detrimental to EAdsRNA To determine which of the nine amino-acid...
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Báo cáo y học: "Evolution of the HIV-1 nef gene in HLA-B*57 Positive Elite Suppressors" pdf
... However, in all four patients, we saw very few non-synonymous mutations and a comparable frequency of synonymous mutations in both genes This analysis is technically challenging because it involves the ... seen at the HLA-B*57 restricted epitope KF9 (Nef 82-90) in ES3 and ES8 (Figure 1) In both patients, the majority of the plasma clones were different at or amino acids from the majority of the proviral ... early and late time points, there did not appear to be significant evolution in any of the epitopes in either compartment over a year period In order to determine whether evolution was occurring...
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Báo cáo khoa học: Evolution of the teleostean zona pellucida gene inferred from the egg envelope protein genes of the Japanese eel, Anguilla japonica potx
... we discuss the evolution of fish egg envelope genes using phylogenetic analysis Results Purification of envelope proteins from Japanese eel The unfertilized egg envelope proteins of Japanese eel ... (ML) trees of the nucleotide sequences of the zp genes of Teleostei (A) Phylogenetic tree of teleostean zp genes The subfamilies of zp genes are labelled (ZPB, ZPC and ZPX) (B) A tree of teleostean ... out In the present study, we describe the purification of the egg envelope proteins from unfertilized egg envelopes of Japanese eel, cloning of the corresponding cDNAs and analysis of their expression...
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Báo cáo khoa học: Directed evolution of a histone acetyltransferase – enhancing thermostability, whilst maintaining catalytic activity and substrate specificity doc
... catalytic domain of the human HAT P ⁄ CAF, without affecting its catalytic activity or substrate specificity P ⁄ CAF, p300 ⁄ CBP-associating factor, is a trans criptional coactivator with a variable N-terminal, ... Continuous HAT assay Progress curves as a measure of HAT activity of wild-type and mutant P ⁄ CAFs were followed using a coupled enzyme assay [62] HAT activity generates CoA as a byproduct that is ... Stabilizing a human histone acetyltransferase H Leemhuis et al 30 Herrera JE, Bergel M, Yang XJ, Nakatani Y & Bustin M (1997) The histone acetyltransferase activity of human GCN5 and PCAF is stabilized...
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Báo cáo khoa học: Directed evolution of a glutaryl acylase into an adipyl acylase potx
... wild-type and mutant Pseudomonas SY-77 glutaryl acylase on glutaryl- 7-ACA and adipyl- 7-ADCA Shown are values of mean ± S.D of at least four independent measurements Ó FEBS 2002 4500 C F Sio et al (Eur ... other hand, the mutants are not improved in catalysis of glutaryl- 7-ACA as shown by the lower kcat of all mutants and the lower affinity of mutant Y178H for the glutaryl substrate A parameter ... The substrate specificity of the mutant acylases was analysed by determining the kinetic parameters on glutaryl- 7-ACA and adipyl- 7-ADCA, and comparing them to the kinetic parameters of the wild-type...
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Báo cáo hóa học: " An FIR Notch Filter for Adaptive Filtering of a Sinusoid in Correlated Noise" potx
... which are of the orders of 1/N, 1, and N Hence, the amplitude A can be approximately obtained as A= a (N + 1) a sin θs (A. 9) O Kukrer and A Hocanin The covariance σXY can be obtained as σXY ... constrained poles and zeros,” IEEE Transactions on Acoustics, Speech, and Signal Processing, vol 33, no 4, pp 983–996, 1985 [6] P Stoica and A Nehorai, “Performance analysis of an adaptive notch ... A Hocanin and O Kukrer, “Estimation of the frequency and waveform of a single-tone sinusoid using an offline-optimized adaptive filter,” in Proceedings of IEEE International Conference on Acoustics,...
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Báo cáo sinh học: "Adaptive evolution of centromere proteins in plants and animals" ppt
... Comparisons of CENP-C proteins in animals, yeast and plants The CENPC motif and conserved regions found at the termini of CENP-C proteins are indicated For pairwise comparisons of protein-coding sequences, ... DNAbinding proteins would maintain an interface with the conserved kinetochore machinery Indeed, regions of CENP-C that show evidence of positive selection include DNAbinding and specificity regions, in ... adaptive evolution of DNA-binding specificity in these regions Targeting of native CENP-C proteins depends on other centromere proteins that vary according to species [45], but the dependence of CENP-Cs...
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Comparative genome and phenotypic analysis of Clostridium difficile 027 strains provides insight into the evolution of a hypervirulent bacterium" ppsx
... pinpointing the genetic and phenotypic attributes that may explain the emergence of the hypervirulent 027 strain and contribute in general to our understanding of the evolution of bacterial virulence Materials ... [22] The genomic sequences of strains CD196 and R20291 were compared against the database of strain 630 proteins by blastx, and a CDS feature in the query genome was created when a hit of over ... performed the data analysis MG and MRP performed toxin B toxicity assays, LD performed motility and autoagglutination assays, EV performed MIC assays, MM performed PCR analysis, and DNG provided strains...
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Báo cáo y học: " Evolution of subtype C HIV-1 Env in a slowly progressing Zambian infant" potx
... morbidity and mortality HIV-1 infected children account for 20% of all HIV-1 related deaths; 7% of individuals living with HIV-1 infection, and 16% of new HIV-1 infections annually [4] In sub-Saharan ... homogeneity, and rapid viral replication kinetics, have been correlated with perinatal HIV-1 transmission [6-8] In addition, advanced maternal disease status, lack of drug therapy, and lack of breast-feeding ... A, Rouzioux C, Le Deist F, Fukunaga K, Caniglia M, Jacomet C, Messiah A, Griscelli C: Longitudinal study of 94 symptomatic infants with perinatally acquired human immunodeficiency virus infection...
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Báo cáo y học: " Evolution of the uniquely adaptable lentiviral envelope in a natural reservoir host" potx
... within a single animal can vary by greater than 35% at the aa level The ranges of aa diversity in some intra-host pairwise SIVsm V1V2 sequence comparisons in this study rival that of inter-animal ... glycosylated in naturally infected SMs Presumably, continually evolving antibody responses in these natural hosts maintain a highly glycosylated surface protein, albeit without effectively suppressing ... (0-2.25 years) FBO FDO FFJ FJO FQI Figure Viral load quantification for five naturally infected sooty mangabeys Viral load quantification for five naturally infected sooty mangabeys Viral RNA in plasma...
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Báo cáo y học: "The role of emergency medicine physicians in trauma care in North America: evolution of a specialty" pps
... optimal care of trauma patients As early as 1966 the role of physicians staffing emergency rooms in the care of trauma patients was discussed as an important element in preventable trauma death ... training allows exposure to trauma only as part of their general EM training, particularly if a separate trauma team responds for trauma activations In smaller North American hospitals that are ... new European EMP's may pursue advanced training in Critical Care leading to a specialty board exam The role of these physicians in trauma care is not well known In US and Canadian trauma centers...
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