Identification of novel cytosolic binding partners of the neural cell adhesion molecule NCAM and functional analysis of these interactions
Identification of novel cytosolic binding partners of the neural cell adhesion molecule NCAM and functional analysis of these interactions
... influence of kinesin-1 on the delivery of NCAM to the cell surface in CHO cells 55 Fig 12: Functional analysis of the influence of kinesin-1 on the delivery of NCAMCT to the cell surface ... kinesin-1 and kinesin light chain 13 Fig 4: Analysis of the purification fractions and the concentrate of hNCAM180ID 41 Fig 5: Analysis of the purification fractions and the concentrate of hNCAM140ID ... Human NCAM Human NCAM with deleted intracellular domain Intracellular domain of human NCAM isoform 140 Human NCAM isoform 180 Intracellular domain of human NCAM isoform 180 Extracellular domain of...
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Báo cáo khoa học: A novel retinol-binding protein in the retina of the swallowtail butterfly, Papilio xuthus docx
... analyses of the protein As the protein is a novel species of the hydrophobicligand-binding protein and solely binds 3-hydroxyretinol as an intrinsic ligand, we termed this protein the Papilio retinol-binding ... Brilliant Blue staining of the gel indicates that the fluorescing protein is one of the major components of soluble proteins in the crude extract The surface of the fluorescing protein carries negative ... Longitudinal sections of the intact (left) and the distal portion (right) of the Papilio eye (B) Native PAGE indicating the distribution of Papilio RBP in the distal and proximal portions of the retina...
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Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx
... like the Trp residue of the 183 Conformation, ligand binding and distribution of nematode protein Ag-NPA-1 R Jordanova et al Fig Immunohistological localization of Ag-NPA-1 in adult A galli and ... ligand binding and distribution of nematode protein Ag-NPA-1 Fig Immunogold electron microscopic localization of Ag-NPA-1 in a male A galli worm (A) Section of the striate musculature (mu) and the ... emission upon binding characterize the binding sites of NPAs as highly nonpolar and completely isolated from the solvent Here we analyze the conformational and functional properties of Ag-NPA-1 as...
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Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx
... to the cytosol Here we confirm and extend earlier studies of these AAA-peroxins and give a further detailed functional analysis of their cassette structure and interaction The interaction of Pex1p ... for the Pex1p Pex6p interaction and their functional role in peroxisome biogenesis Results The contribution of Pex1p and Pex6p to peroxisomal biogenesis is well established on the basis of the ... revealed the presence of Pex6p in the precipitate of full-length Pex1p ProtA and also the presence of Pex1p in the full-length Pex6p ProtA-precipitate (Fig 1C,D) These data confirm the in vivo interaction...
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Báo cáo khoa học: Cloning and functional analysis of 5¢-upstream region of the Pokemon gene pptx
... Results Cloning of the 5¢-upstream region of the Pokemon gene To identify the regulatory sequences that control expression of the Pokemon gene, a 2204-bp section of the 5¢-upstream region of the Pokemon ... mechanisms of the Pokemon gene Computer analysis of putative transcription factor-binding sites For a rough understanding of the regulation of the Pokemon gene, the 2204-bp section of its 5¢-upstream region ... 2008 The Authors Journal compilation ª 2008 FEBS 1861 Analysis of upstream region of the Pokemon gene Y Yang et al Fig Nucleotide sequence of the 5¢-upstream region of the Pokemon gene The upstream...
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Báo cáo khoa học: A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea docx
... counteracting effects of two natural osmolytes namely TMAO and monosodium glutamate against the denaturing effects of urea on the bacterial cell division protein, FtsZ TMAO was chosen because of its ability ... effects of urea on FtsZ Interestingly TMAO also enhanced the bundling of FtsZ protofilaments and suppressed GTPase activity of native FtsZ suggesting that osmolytes can modulate assembly and bundling ... the binding of GTP to FtsZ TMAO enhanced aggregation of FtsZ that could also reduce the GTPase activity of FtsZ 2765 TMAO effects on FtsZ A Mukherjee et al A B Fig Effects of TMAO on the GTPase...
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Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx
... that the F-11 cells not only express the rat VRL-1, but also the mouse VRL-1 and that the mouse variant is derived from the F-11 parental cell line N18TG2 This finding is of interest when VRL-1 ... A, B and D of F-11 cells again separated into two bands The slower migrating band always corresponded to the rat brain control, and the faster migrating band to the N18TG2 control (Fig 5) The ... characteristics of the capsaicin sensitive Vanilloid receptor VR1 transiently transfected in the rat dorsal root ganglia derived cell line F-11, a hybridoma of mouse neuroblastoma and rat dorsal root ganglion...
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Báo cáo khoa học: " A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR" potx
... suspension for h at 4°C and virus attachment was subsequently measured using RT-PCR B) MOI TCID50 /cell of CVB2O was incubated as described in A) at 4°C or at room temperature and attached virus was measured ... demonstrating that incubation at a higher temperature reduced unspecific attachment of this virus to CHO cells, while attachment to CHO-CAR, CHO-DAF and HeLa remained at the same level Thus, a significant ... amount of bound radioactivity is used as a measurement of the viral attachment capacity In this article, we demonstrate that RT-PCR is an alternative, rapid and efficient method to study viral...
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Báo cáo y học: "Survivors of war in the Northern Kosovo (II): baseline clinical and functional assessment and lasting effects on the health of a vulnerable population" ppsx
... Survivors of war in the Northern Kosovo (II): baseline clinical and functional assessment and lasting effects on the health of a vulnerable population Conflict and Health 2010 4:16 Submit your next manuscript ... physical functioning and activity, participation in social life and environmental factors was obtained in further interviews using a questionnaire based on the WHO International Classification ... Vergara A, Agani F, Gotway CA: Mental health, social functioning, and attitudes of Kosovar Albanians following the war in Kosovo Jama 2000, 284(5):569-577 13 American Association for the Advancement...
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Regulation and functional analysis of the tumor suppressor gene product, p53
... synthesize first 29 strand cDNA as well as whole p53 gene The 5’ end of the forward primer starts from the start codon of p53 gene and in case of reverse primer the 5’ end starts just after the ... Regulation of p53 dependent DNA-repair target gene promoters 60 by p53Pro and p53Arg variants VI 3.2.2.1 Expression of Gadd45 promoter in presence of either p53Pro 60 or p53Arg 3.2.2.2 Expression of p53R2 ... 1992) Because of these properties, p53 gene is considered as a “guardian of the genome” p53 protein lies at the heart of stress response pathways that prevent the growth and survival of potentially...
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Báo cáo Y học: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases pot
... report on the isolation of four new monoterpene synthase cDNAs by random sequencing of a flavedo-derived cDNA library of C limon and their characterization by functional expression in Escherichia ... sabinene, a-terpinene (E )-b-ocimene, terpinolene, linalool and a-terpineol cDNA isolation and sequencing Random sequencing of a cDNA library made from mRNA isolated from the peel of young lemon fruits ... of identity of the lemon monoterpene synthases indicate that they should be grouped within the tpsb clade of the angiosperm monoterpene synthases (Fig 1, and Table 1) [34] Although the four lemon...
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Báo cáo khoa học: Structural and functional analysis of ataxin-2 and ataxin-3 potx
... b5 strand) of D3 and F27/Y289 (b2 strand), L67/M324, V70/I327 and L72/L328 (all in b4 strand) of B The second cluster consists of P6/M267, L10/ L271 (both in a-helix), V18/C279 (b1 strand), L32/F293 ... solution structures of the UBL domain of hHR23A/B bound to a UIM peptide of S5a [99,119] could be used to model the complex of hHR23A/B and ataxin-3 Similarly, the complex of a UIM of ataxin-3 with ... detailed analysis of ataxin-2 homologues including the yeast homologue Pbp1, using a structurebased multiple sequence alignment of Sm and Sm-like proteins and a 3D model of the Lsm domain of ataxin-2...
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Báo cáo khoa học: "Phenotypic and functional analysis of bovine γδ lymphocytes" pps
... expression of WC1N3 and WC1-N4 isoforms and expression of families of the γδ TCR that express determinants TCR1-N6, TCR1N7, or TCR1-N6 and -N7 Only a subset of WC1- γδ T cells expressed a form of TCR1 ... Determination of the antigenic phenotype and frequency of subsets of WC1+ and WC1- γδ T cells in peripheral blood and lymphoid tissues: i) Flow cytometric analysis: Analysis of the tissue distribution of ... selected time points and processed for FC and preparation of mRNA For sorting, the cells were labeled Phenotypic and functional analysis of bovine γδ lymphocytes Table List of mAbs used in this...
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Báo cáo y học: " Genetic and functional analysis of HIV-1 Rev Responsive Element (RRE) sequences from North-India" pdf
... available on HIV-1 subtype C RRE genetic and functional characteristics In the present study, we present in-depth genetic and functional analysis of RRE sequences from a cohort of 13 HIV-1 infected ... between HIV-1 subtypes B and C and with probably other subtypes as well The analysis of intra-subtype divergence (genetic distance from Page of Indian consensus sequence) and diversity (intra-subtype ... amounts of Rev protein and subjected to EMSA as described earlier [18] Results and discussion Analysis of RRE nucleotide sequences 83 sequences of subtype B (from USA, Japan, Mayanmar, France and...
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Báo cáo y học: "Insulin augments tumor necrosis factor-alpha stimulated expression of vascular cell adhesion molecule-1 in vascular endothelial cells." pot
... Insulin augments tumor necrosis factor-alpha stimulated expression of vascular cell adhesion molecule-1 in vascular endothelial cells Daniel Z Mackesy1 and Marc L Goalstone1,2,* Department of ... pathogenesis of atherosclerosis by augmenting the effects of inflammatory cytokines, thereby significantly increasing the expression of CAMs [6-11] One such cytokine is tumor necrosis factor-alpha ... endothelial cells of the intima Activated immune cells translocate into the vascular tissue and away from the circulatory system whereby they secrete inflammatory cytokines and induce the sequelae of inflammation...
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Từ khóa: rnai technology to block the expression of molecules relevant to metastasis the cell adhesion molecule ceacam1 as an instructive examplegenomics discovery and functional analysis of genessignificance of cell adhesion to biotechnological and biomedical applicationsbrachypodium distachyon a new model system for structural and functional analysis of grass genomesthe plant cell wall—structural aspects and biotechnological developmentsbioreactor engineering regenerating the dynamic cell microenvironment tal dvir and smadar cohenthe stem cell laboratory design equipment and oversightthe blood cerebrospinal fluid barrier structure and functional significancethe identification of novel biomarkersprbp pdedelta pull down assays for identifying binding partners and characterizing the determinants of high affinity binding to prbp pdedeltaa unified statistical model for the identification of english basenptwo wheeled motorcycles and mopeds driver operated controls including the identification of controls tell tales and indicators requirements in type approvalidentification of the individuals or companies with which the related party transactions have been carried out specifying the nature of the relationship with each party involvedthe identification of opportunities and threatscost and the identification of legally relevant state practiceBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Nghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀM